Phosphoglucomutase‐1 subtyping of human bloodstains on ultrathin‐layer polyacrylamide gels

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A method is described for obtaining nondistorted, reproducible phosphoglucomutase‐1 subtyping patterns from fresh human bloodstains by ultrathin‐layer polyacrylamide gel isoelectric focusing. Electrofocusing of phosphoglucomutase on 0.2 mm thick gels took 52 and 80 min on the Cold Focus Apparatus and Ultrophor, respectively, when the distance separating the electrodes was 8.0 cm, whereas a 9.5 cm distance between electrode wicks required 95 min on the Multiphor to complete isoelectric focusing. The gels with 8.0 cm between electrode wicks provided sharper band patterns compared with the gels with 9.5 cm between electrode wicks.

Original languageEnglish
Pages (from-to)165-167
Number of pages3
JournalELECTROPHORESIS
Volume5
Issue number3
DOIs
StatePublished - 1 Jan 1984

Fingerprint

Capillary Action
Electrodes
Isoelectric Focusing
Gels
Phosphoglucomutase
polyacrylamide gels

Cite this

@article{491d94d06e45472aa2ab74590cb13473,
title = "Phosphoglucomutase‐1 subtyping of human bloodstains on ultrathin‐layer polyacrylamide gels",
abstract = "A method is described for obtaining nondistorted, reproducible phosphoglucomutase‐1 subtyping patterns from fresh human bloodstains by ultrathin‐layer polyacrylamide gel isoelectric focusing. Electrofocusing of phosphoglucomutase on 0.2 mm thick gels took 52 and 80 min on the Cold Focus Apparatus and Ultrophor, respectively, when the distance separating the electrodes was 8.0 cm, whereas a 9.5 cm distance between electrode wicks required 95 min on the Multiphor to complete isoelectric focusing. The gels with 8.0 cm between electrode wicks provided sharper band patterns compared with the gels with 9.5 cm between electrode wicks.",
author = "Bruce Budowle",
year = "1984",
month = "1",
day = "1",
doi = "10.1002/elps.1150050309",
language = "English",
volume = "5",
pages = "165--167",
journal = "ELECTROPHORESIS",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "3",

}

Phosphoglucomutase‐1 subtyping of human bloodstains on ultrathin‐layer polyacrylamide gels. / Budowle, Bruce.

In: ELECTROPHORESIS, Vol. 5, No. 3, 01.01.1984, p. 165-167.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Phosphoglucomutase‐1 subtyping of human bloodstains on ultrathin‐layer polyacrylamide gels

AU - Budowle, Bruce

PY - 1984/1/1

Y1 - 1984/1/1

N2 - A method is described for obtaining nondistorted, reproducible phosphoglucomutase‐1 subtyping patterns from fresh human bloodstains by ultrathin‐layer polyacrylamide gel isoelectric focusing. Electrofocusing of phosphoglucomutase on 0.2 mm thick gels took 52 and 80 min on the Cold Focus Apparatus and Ultrophor, respectively, when the distance separating the electrodes was 8.0 cm, whereas a 9.5 cm distance between electrode wicks required 95 min on the Multiphor to complete isoelectric focusing. The gels with 8.0 cm between electrode wicks provided sharper band patterns compared with the gels with 9.5 cm between electrode wicks.

AB - A method is described for obtaining nondistorted, reproducible phosphoglucomutase‐1 subtyping patterns from fresh human bloodstains by ultrathin‐layer polyacrylamide gel isoelectric focusing. Electrofocusing of phosphoglucomutase on 0.2 mm thick gels took 52 and 80 min on the Cold Focus Apparatus and Ultrophor, respectively, when the distance separating the electrodes was 8.0 cm, whereas a 9.5 cm distance between electrode wicks required 95 min on the Multiphor to complete isoelectric focusing. The gels with 8.0 cm between electrode wicks provided sharper band patterns compared with the gels with 9.5 cm between electrode wicks.

UR - http://www.scopus.com/inward/record.url?scp=0021228662&partnerID=8YFLogxK

U2 - 10.1002/elps.1150050309

DO - 10.1002/elps.1150050309

M3 - Article

AN - SCOPUS:0021228662

VL - 5

SP - 165

EP - 167

JO - ELECTROPHORESIS

JF - ELECTROPHORESIS

SN - 0173-0835

IS - 3

ER -