Phosphoglucomutase‐1 subtyping of human bloodstains on ultrathin‐layer polyacrylamide gels

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A method is described for obtaining nondistorted, reproducible phosphoglucomutase‐1 subtyping patterns from fresh human bloodstains by ultrathin‐layer polyacrylamide gel isoelectric focusing. Electrofocusing of phosphoglucomutase on 0.2 mm thick gels took 52 and 80 min on the Cold Focus Apparatus and Ultrophor, respectively, when the distance separating the electrodes was 8.0 cm, whereas a 9.5 cm distance between electrode wicks required 95 min on the Multiphor to complete isoelectric focusing. The gels with 8.0 cm between electrode wicks provided sharper band patterns compared with the gels with 9.5 cm between electrode wicks.

Original languageEnglish
Pages (from-to)165-167
Number of pages3
JournalELECTROPHORESIS
Volume5
Issue number3
DOIs
StatePublished - 1 Jan 1984

Fingerprint Dive into the research topics of 'Phosphoglucomutase‐1 subtyping of human bloodstains on ultrathin‐layer polyacrylamide gels'. Together they form a unique fingerprint.

  • Cite this