Phenolic compounds protect cultured hippocampal neurons against ethanol-withdrawal induced oxidative stress

Katalin Prokai-Tatrai, Laszlo Prokai, James W. Simpkins, Marianna E. Jung

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Ethanol withdrawal is linked to elevated oxidative damage to neurons. Here we report our findings on the contribution of phenolic antioxidants (17β-estradiol, p-octylphenol and 2,6-di-tert-butyl-4-methylphenol) to counterbalance sudden ethanol withdrawal-initiated oxidative events in hippocampus-derived cultured HT-22 cells. We showed that ethanol withdrawal for 4 h after 24-h ethanol treatment provoked greater levels of oxidative damage than the preceding ethanol exposure. Phenolic antioxidant treatment either during ethanol exposure or ethanol withdrawal only, however, dose-dependently reversed cellular oxidative damage, as demonstrated by the significantly enhanced cell viability, reduced malondialdehyde production and protein carbonylation, compared to untreated cells. Interestingly, the antioxidant treatment schedule had no significant impact on the observed neuroprotection. In addition, the efficacy of the three phenolic compounds was practically equipotent in protecting HT-22 cells in spite of predictions based on an in silico study and a cell free assay of lipid peroxidation. This finding implies that free-radical scavenging may not be the sole factor responsible for the observed neuroprotection and warrants further studies to establish, whether the HT-22 line is indeed a suitable model for in vitro screening of antioxidants against EW-related neuronal damage.

Original languageEnglish
Pages (from-to)1773-1787
Number of pages15
JournalInternational journal of molecular sciences
Issue number4
StatePublished - Apr 2009


  • Ethanol withdrawal
  • Lipid peroxidation
  • Oxidative stress
  • Phenolic antioxidant
  • Protein carbonylation


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