Phase-modulation fluorometry using a frequency-doubled pulsed laser diode light source

Klaus W. Berndt, Ignacy Gryczynski, Joseph R. Lakowicz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

We used the Hamamatsu model PLP-01 picosecond light pulser as a 413-nm excitation light source for frequency-domain fluorescence measurements. In comparison with sync-pumped/cavity dumped/frequency-doubled dye lasers, the 413-nm PLP-01 shows a longer FWHM (40 ps), a similar pulse repetition rate (up to 10 MHz), much less output power at a fixed wavelength (0.44 mW peak, 220 nW maximum average power), but is less expensive, small-sized, and easy to handle. Using the PLP-01, we have been able to perform fluorescence measurements up to an upper modulation frequency of about 2000 MHz, and to resolve mixtures of fluorophores exhibiting different lifetimes. During our tests, we observed remarkable and lasting (2 h) time drifts between the optical output and the electrical trigger input or output. At present, work is in progress at Hamamatsu to eliminate these drifts.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsJoseph F. Lakowicz
PublisherPubl by Int Soc for Optical Engineering
Pages253-261
Number of pages9
ISBN (Print)0819402451
StatePublished - 1 Dec 1990
EventTime-Resolved Laser Spectroscopy in Biochemistry II - Los Angeles, CA, USA
Duration: 15 Jan 199017 Jan 1990

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1204 pt 1
ISSN (Print)0277-786X

Other

OtherTime-Resolved Laser Spectroscopy in Biochemistry II
CityLos Angeles, CA, USA
Period15/01/9017/01/90

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    Berndt, K. W., Gryczynski, I., & Lakowicz, J. R. (1990). Phase-modulation fluorometry using a frequency-doubled pulsed laser diode light source. In J. F. Lakowicz (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (pp. 253-261). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1204 pt 1). Publ by Int Soc for Optical Engineering.