Permeability of the rabbit colon in vitro

Thomas Yorio, P. J. Bentley

Research output: Contribution to journalArticlepeer-review


The rabbit colon, in vitro as everted sac or diaphragm preparations, exhibits a transmural PD, as high as 70 mV, a short circuit current (SCC) of 100 to 150 μA cm-2 and a resistance of 300-500 Ω.cm2. It maintains these functions for at least 3 h. The SCC can be abolished by amiloride or increased by amphotericin B. Na, Cl, and K flux measurements showed a net influx (mucosa to serosa) of Na and a net efflux of K. The SCC can be accounted for by the movements of these ions. The SCC in the presence of amphotericin B was nearly equivalent to the net Na flux. Amiloride abolished the net Na transfer but did not have a significant effect on the K. The permeability coefficient (Ktransx107) for water was 5,000 cm S-1 while that for urea was 26 cm s-1. The rabbit provides a viable preparation for in vitro permeability studies of colonic function and the present observations appear to be consistent with its physiological role.

Original languageEnglish
JournalAmerican Journal of Physiology
Issue number1
StatePublished - 1 Jan 1977


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