Peptide domain involved in the interaction between membrane protein and nucleocapsid protein of SARS-associated coronavirus

Xiaonan Fang, Linbai Ye, Khalid A. Timani, Shanshan Li, Yingchun Zen, Meng Zhao, Hong Zheng, Zhenghui Wu

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Severe acute respiratory syndrome (SARS) is an emerging infectious disease associated with a novel coronavirus (CoV) that was identified and molecularly characterized in 2003. Previous studies on various coronaviruses indicate that protein-protein interactions amongst various coronavirus proteins are critical for viral assembly and morphogenesis. It is necessary to elucidate the molecular mechanism of SARS-CoV replication and rationalize the anti-SARS therapeutic intervention. In this study, we employed an in vitro GST pull-down assay to investigate the interaction between the membrane (M) and the nucleocapsid (N) proteins. Our results show that the interaction between the M and N proteins does take place in vitro. Moreover, we provide an evidence that 12 amino acids domain (194-205) in the M protein is responsible for binding to N protein. Our work will help shed light on the molecular mechanism of the virus assembly and provide valuable information pertaining to rationalization of future anti-viral strategies.

Original languageEnglish
Pages (from-to)381-385
Number of pages5
JournalJournal of Biochemistry and Molecular Biology
Volume38
Issue number4
StatePublished - 1 Dec 2005

Fingerprint

Nucleocapsid Proteins
Severe Acute Respiratory Syndrome
Coronavirus
Membrane Proteins
Peptides
Virus Assembly
Proteins
Emerging Communicable Diseases
Nucleocapsid
Morphogenesis
Protein Domains
Viruses
Assays
Amino Acids
Membranes

Keywords

  • Membrane protein
  • Nucleocapsid protein
  • Protein interaction and GST resin pull-down assay
  • SARS-CoV

Cite this

Fang, Xiaonan ; Ye, Linbai ; Timani, Khalid A. ; Li, Shanshan ; Zen, Yingchun ; Zhao, Meng ; Zheng, Hong ; Wu, Zhenghui. / Peptide domain involved in the interaction between membrane protein and nucleocapsid protein of SARS-associated coronavirus. In: Journal of Biochemistry and Molecular Biology. 2005 ; Vol. 38, No. 4. pp. 381-385.
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Peptide domain involved in the interaction between membrane protein and nucleocapsid protein of SARS-associated coronavirus. / Fang, Xiaonan; Ye, Linbai; Timani, Khalid A.; Li, Shanshan; Zen, Yingchun; Zhao, Meng; Zheng, Hong; Wu, Zhenghui.

In: Journal of Biochemistry and Molecular Biology, Vol. 38, No. 4, 01.12.2005, p. 381-385.

Research output: Contribution to journalArticle

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T1 - Peptide domain involved in the interaction between membrane protein and nucleocapsid protein of SARS-associated coronavirus

AU - Fang, Xiaonan

AU - Ye, Linbai

AU - Timani, Khalid A.

AU - Li, Shanshan

AU - Zen, Yingchun

AU - Zhao, Meng

AU - Zheng, Hong

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AB - Severe acute respiratory syndrome (SARS) is an emerging infectious disease associated with a novel coronavirus (CoV) that was identified and molecularly characterized in 2003. Previous studies on various coronaviruses indicate that protein-protein interactions amongst various coronavirus proteins are critical for viral assembly and morphogenesis. It is necessary to elucidate the molecular mechanism of SARS-CoV replication and rationalize the anti-SARS therapeutic intervention. In this study, we employed an in vitro GST pull-down assay to investigate the interaction between the membrane (M) and the nucleocapsid (N) proteins. Our results show that the interaction between the M and N proteins does take place in vitro. Moreover, we provide an evidence that 12 amino acids domain (194-205) in the M protein is responsible for binding to N protein. Our work will help shed light on the molecular mechanism of the virus assembly and provide valuable information pertaining to rationalization of future anti-viral strategies.

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KW - Nucleocapsid protein

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