pcDNAL1 genetic immunization can induce specific cell-mediated immune responses in C57BL/6 mice

Jianming Song, Xiang Le Sun, Tianju Liu, Yili Wang, Lüsheng Si

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Objective. To investigate the specific cell-mediated immune efficacy of the an HPV16 prophylactic vaccine. Methods. C57BL/6 mice were randomly divided into 3 groups: experimental group I (treated with pcDNA L1), control group II (treated with pcDNA3. 1) and control group III (treated with PBS buffer). The mice were immunized three times during a three-week interval. Ten to fourteen days after the third inoculation, a footpad swelling test was used to detect delayed-type hypersensitivity (DTH) responses. Antigen-specific splenocyte proliferation assay and quantitation of IFN-γ cells in splenocytes were performed by FACS assay. Results. In the experimental group, splenocytes actively proliferated after stimulation with HPV16 VLP, and had developed a markedly larger amount of CD8+ IFN-γ+ cells, which is an index for special CTL. Also, the footpad was significantly thickened upon inoculation with HPV16 VLP. Conclusion. Naked DNA vaccine of HPV16 L1 can induce specific cell-mediated immune responses in mice, which should be considered for evaluation of HPV16 DNA vaccine feasibility.

Original languageEnglish
Pages (from-to)1697-1700
Number of pages4
JournalChinese Medical Journal
Volume115
Issue number11
StatePublished - 1 Nov 2002

Keywords

  • DNA vaccine
  • HPV16
  • Prophylactic vaccine

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    Song, J., Sun, X. L., Liu, T., Wang, Y., & Si, L. (2002). pcDNAL1 genetic immunization can induce specific cell-mediated immune responses in C57BL/6 mice. Chinese Medical Journal, 115(11), 1697-1700.