Parsing apart the contributors of mitochondrial DNA mixtures with massively parallel sequencing data

Jennifer D. Churchill, Monika Stoljarova, Jonathan L. King, Bruce Budowle

Research output: Contribution to journalArticle

3 Scopus citations


The data generated from massively parallel sequencing (MPS) technologies offer new avenues for the analysis and interpretation of mitochondrial DNA (mtDNA) mixture samples. Two-person mixtures at nuclear DNA ratios of 1:1, 1:5, 1:10, and 1:20 were prepared, and the mitochondrial genomes of these samples were sequenced using the Precision ID mtDNA Whole Genome Panel, Ion Chef, and Ion PGM sequencer (Thermo Fisher Scientific) workflow. The generated data were used to evaluate deconvolution of the mixed mtDNA samples. Results showed successful identification of the major contributor's haplotype in each of the mixed samples that were evaluated.

Original languageEnglish
Pages (from-to)e439-e441
JournalForensic Science International: Genetics Supplement Series
StatePublished - Dec 2017



  • Ion PGM
  • Massively parallel sequencing
  • Mitochondrial DNA
  • Mixtures

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