Abstract
The data generated from massively parallel sequencing (MPS) technologies offer new avenues for the analysis and interpretation of mitochondrial DNA (mtDNA) mixture samples. Two-person mixtures at nuclear DNA ratios of 1:1, 1:5, 1:10, and 1:20 were prepared, and the mitochondrial genomes of these samples were sequenced using the Precision ID mtDNA Whole Genome Panel, Ion Chef, and Ion PGM sequencer (Thermo Fisher Scientific) workflow. The generated data were used to evaluate deconvolution of the mixed mtDNA samples. Results showed successful identification of the major contributor's haplotype in each of the mixed samples that were evaluated.
| Original language | English |
|---|---|
| Pages (from-to) | e439-e441 |
| Journal | Forensic Science International: Genetics Supplement Series |
| Volume | 6 |
| DOIs | |
| State | Published - 1 Dec 2017 |
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Keywords
- Ion PGM
- Massively parallel sequencing
- Mitochondrial DNA
- Mixtures
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Parsing apart the contributors of mitochondrial DNA mixtures with massively parallel sequencing data. / Cihlar, Jennifer Churchill; Stoljarova, Monika; King, Jonathan L.; Budowle, Bruce.
In: Forensic Science International: Genetics Supplement Series, Vol. 6, 01.12.2017, p. e439-e441.Research output: Contribution to journal › Article
TY - JOUR
T1 - Parsing apart the contributors of mitochondrial DNA mixtures with massively parallel sequencing data
AU - Cihlar, Jennifer Churchill
AU - Stoljarova, Monika
AU - King, Jonathan L.
AU - Budowle, Bruce
PY - 2017/12/1
Y1 - 2017/12/1
N2 - The data generated from massively parallel sequencing (MPS) technologies offer new avenues for the analysis and interpretation of mitochondrial DNA (mtDNA) mixture samples. Two-person mixtures at nuclear DNA ratios of 1:1, 1:5, 1:10, and 1:20 were prepared, and the mitochondrial genomes of these samples were sequenced using the Precision ID mtDNA Whole Genome Panel, Ion Chef, and Ion PGM sequencer (Thermo Fisher Scientific) workflow. The generated data were used to evaluate deconvolution of the mixed mtDNA samples. Results showed successful identification of the major contributor's haplotype in each of the mixed samples that were evaluated.
AB - The data generated from massively parallel sequencing (MPS) technologies offer new avenues for the analysis and interpretation of mitochondrial DNA (mtDNA) mixture samples. Two-person mixtures at nuclear DNA ratios of 1:1, 1:5, 1:10, and 1:20 were prepared, and the mitochondrial genomes of these samples were sequenced using the Precision ID mtDNA Whole Genome Panel, Ion Chef, and Ion PGM sequencer (Thermo Fisher Scientific) workflow. The generated data were used to evaluate deconvolution of the mixed mtDNA samples. Results showed successful identification of the major contributor's haplotype in each of the mixed samples that were evaluated.
KW - Ion PGM
KW - Massively parallel sequencing
KW - Mitochondrial DNA
KW - Mixtures
UR - http://www.scopus.com/inward/record.url?scp=85029740705&partnerID=8YFLogxK
U2 - 10.1016/j.fsigss.2017.09.145
DO - 10.1016/j.fsigss.2017.09.145
M3 - Article
AN - SCOPUS:85029740705
VL - 6
SP - e439-e441
JO - Forensic Science International: Genetics Supplement Series
JF - Forensic Science International: Genetics Supplement Series
SN - 1875-1768
ER -