Orientation and spectral properties of two stilbazolium merocyanine dyes in stretched and unstretched polyvinyl alcohol films

Alina Dudkowiak, Danuta Fra̧ckowiak, Ewa Teślak, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Spectral properties (anisotropy coefficients calculated for absorption, emission and fluorescence decay time) of two stilbazolium merocyanine dyes have been determined to evaluate the applicability of these dyes as sensitizers in photodynamic therapy. The dyes were embedded in an anisotropic polymer matrix. Analysis of the emission decay components measured in polarized light provides information on the interactions of the dye molecules with the polymer matrix being a model of an anisotropic biological system. Different values of the emission anisotropies obtained from various polarized components of fluorescence decays have shown that the orientations of the dye molecules influence their interactions with the polymer. This means that differently oriented dye molecules located in biological systems should exhibit different interactions with membranes. The chain length and type of side groups attached as well as the salt form of the dye molecule were shown to influence the dye-polymer interactions and should be taken into account before the application of merocyanine dyes in medicine. These dyes seem to be promising optical sensors with spectral properties, including the calculated anisotropy coefficients, sensitive to the molecular environment, useful to study orientation and interaction with neighbouring molecules in biological membranes.

Original languageEnglish
Pages (from-to)647-656
Number of pages10
JournalActa Biochimica Polonica
Volume54
Issue number3
StatePublished - 22 Oct 2007

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Polyvinyl Alcohol
Coloring Agents
Anisotropy
Polymers
Molecules
Biological systems
Polymer matrix
Fluorescence
Biological membranes
Biological Models
Photodynamic therapy
Membranes
merocyanine dye
stilbazolium merocyanine
Photochemotherapy
Optical sensors
Light polarization
Chain length
Medicine
Salts

Keywords

  • Anisotropic polymer matrix
  • Photodynamic therapy
  • Stilbazolium merocyanine dyes

Cite this

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title = "Orientation and spectral properties of two stilbazolium merocyanine dyes in stretched and unstretched polyvinyl alcohol films",
abstract = "Spectral properties (anisotropy coefficients calculated for absorption, emission and fluorescence decay time) of two stilbazolium merocyanine dyes have been determined to evaluate the applicability of these dyes as sensitizers in photodynamic therapy. The dyes were embedded in an anisotropic polymer matrix. Analysis of the emission decay components measured in polarized light provides information on the interactions of the dye molecules with the polymer matrix being a model of an anisotropic biological system. Different values of the emission anisotropies obtained from various polarized components of fluorescence decays have shown that the orientations of the dye molecules influence their interactions with the polymer. This means that differently oriented dye molecules located in biological systems should exhibit different interactions with membranes. The chain length and type of side groups attached as well as the salt form of the dye molecule were shown to influence the dye-polymer interactions and should be taken into account before the application of merocyanine dyes in medicine. These dyes seem to be promising optical sensors with spectral properties, including the calculated anisotropy coefficients, sensitive to the molecular environment, useful to study orientation and interaction with neighbouring molecules in biological membranes.",
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Orientation and spectral properties of two stilbazolium merocyanine dyes in stretched and unstretched polyvinyl alcohol films. / Dudkowiak, Alina; Fra̧ckowiak, Danuta; Teślak, Ewa; Gryczynski, Zygmunt; Gryczynski, Ignacy.

In: Acta Biochimica Polonica, Vol. 54, No. 3, 22.10.2007, p. 647-656.

Research output: Contribution to journalArticle

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T1 - Orientation and spectral properties of two stilbazolium merocyanine dyes in stretched and unstretched polyvinyl alcohol films

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AU - Fra̧ckowiak, Danuta

AU - Teślak, Ewa

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

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