One- and two-photon induced fluorescence of pacific blue-labeled human serum albumin deposited on different core size silver colloids

Joanna Lukomska, Ignacy Gryczynski, Joanna Malicka, Slawomir Makowiec, Joseph R. Lakowicz, Zygmunt Gryczynski

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We studied one- and two-photon induced fluorescence of Pacific Blue (PB)-labeled human serum albumin (HSA) in the presence of different size silver colloids. The PB fluorescence emission intensity was observed with small (30-40 nm) and large (about 120 nm) colloids and compared with PB emission in absence of colloids. For the system with a small core size colloids we did not detect any fluorescence enhancement with one-photon excitation and the enhancement observed with two-photon excitation was about 2.5-fold. In contrast, for large silver colloids we observed about a 2-fold increase in PB fluorescence brightness for one-photon excitation, and the enhancement with two-photon excitation excided 13-folds. Much stronger increases in brightness observed with two-photon excitation, compared to one-photon excitation, indicate a dominant role of enhanced local field in fluorescence enhancement on silver colloids in solutions.

Original languageEnglish
Pages (from-to)249-255
Number of pages7
JournalBiopolymers
Volume81
Issue number4
DOIs
StatePublished - Mar 2006

Keywords

  • Enhanced fluorescence
  • Silver colloids
  • Two photon excitation

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