Abstract
We studied one- and two-photon induced fluorescence of Pacific Blue (PB)-labeled human serum albumin (HSA) in the presence of different size silver colloids. The PB fluorescence emission intensity was observed with small (30-40 nm) and large (about 120 nm) colloids and compared with PB emission in absence of colloids. For the system with a small core size colloids we did not detect any fluorescence enhancement with one-photon excitation and the enhancement observed with two-photon excitation was about 2.5-fold. In contrast, for large silver colloids we observed about a 2-fold increase in PB fluorescence brightness for one-photon excitation, and the enhancement with two-photon excitation excided 13-folds. Much stronger increases in brightness observed with two-photon excitation, compared to one-photon excitation, indicate a dominant role of enhanced local field in fluorescence enhancement on silver colloids in solutions.
| Original language | English |
|---|---|
| Pages (from-to) | 249-255 |
| Number of pages | 7 |
| Journal | Biopolymers |
| Volume | 81 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1 Mar 2006 |
Fingerprint
Keywords
- Enhanced fluorescence
- Silver colloids
- Two photon excitation
Cite this
}
One- and two-photon induced fluorescence of pacific blue-labeled human serum albumin deposited on different core size silver colloids. / Lukomska, Joanna; Gryczynski, Ignacy; Malicka, Joanna; Makowiec, Slawomir; Lakowicz, Joseph R.; Gryczynski, Zygmunt.
In: Biopolymers, Vol. 81, No. 4, 01.03.2006, p. 249-255.Research output: Contribution to journal › Article
TY - JOUR
T1 - One- and two-photon induced fluorescence of pacific blue-labeled human serum albumin deposited on different core size silver colloids
AU - Lukomska, Joanna
AU - Gryczynski, Ignacy
AU - Malicka, Joanna
AU - Makowiec, Slawomir
AU - Lakowicz, Joseph R.
AU - Gryczynski, Zygmunt
PY - 2006/3/1
Y1 - 2006/3/1
N2 - We studied one- and two-photon induced fluorescence of Pacific Blue (PB)-labeled human serum albumin (HSA) in the presence of different size silver colloids. The PB fluorescence emission intensity was observed with small (30-40 nm) and large (about 120 nm) colloids and compared with PB emission in absence of colloids. For the system with a small core size colloids we did not detect any fluorescence enhancement with one-photon excitation and the enhancement observed with two-photon excitation was about 2.5-fold. In contrast, for large silver colloids we observed about a 2-fold increase in PB fluorescence brightness for one-photon excitation, and the enhancement with two-photon excitation excided 13-folds. Much stronger increases in brightness observed with two-photon excitation, compared to one-photon excitation, indicate a dominant role of enhanced local field in fluorescence enhancement on silver colloids in solutions.
AB - We studied one- and two-photon induced fluorescence of Pacific Blue (PB)-labeled human serum albumin (HSA) in the presence of different size silver colloids. The PB fluorescence emission intensity was observed with small (30-40 nm) and large (about 120 nm) colloids and compared with PB emission in absence of colloids. For the system with a small core size colloids we did not detect any fluorescence enhancement with one-photon excitation and the enhancement observed with two-photon excitation was about 2.5-fold. In contrast, for large silver colloids we observed about a 2-fold increase in PB fluorescence brightness for one-photon excitation, and the enhancement with two-photon excitation excided 13-folds. Much stronger increases in brightness observed with two-photon excitation, compared to one-photon excitation, indicate a dominant role of enhanced local field in fluorescence enhancement on silver colloids in solutions.
KW - Enhanced fluorescence
KW - Silver colloids
KW - Two photon excitation
UR - http://www.scopus.com/inward/record.url?scp=33645071054&partnerID=8YFLogxK
U2 - 10.1002/bip.20407
DO - 10.1002/bip.20407
M3 - Article
C2 - 16273526
AN - SCOPUS:33645071054
VL - 81
SP - 249
EP - 255
JO - Biopolymers
JF - Biopolymers
SN - 0006-3525
IS - 4
ER -