Oligonucleotide-displaced organic monolayer-protected silver nanoparticles and enhanced luminescence of their salted aggregates

Jian Zhang; Joanna Malicka; Ignacy Gryczynski; Joseph R. Lakowicz

doi:10.1016/j.ab.2004.04.001

Oligonucleotide-displaced organic monolayer-protected silver nanoparticles and enhanced luminescence of their salted aggregates

Jian Zhang, Joanna Malicka, Ignacy Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

N-(2-mercaptopropionyl)glycine (tiopronin) monolayer-protected silver particles were partially displaced by single-stranded oligonucleotides through ligand exchanges. The oligonucleotide-displaced particles could be hybridized with complementary fluorophore-labeled oligonucleotides. Both the oligonucleotide-displaced and hybridized particles could be aggregated by electrostatic interactions with salt in buffer solution, and the aggregates displayed enhanced luminescence from fluorophores. This result suggests the possible application of surface-enhanced fluorescence from metallic nanoparticle aggregation for DNA detection.

Original language	English
Pages (from-to)	81-86
Number of pages	6
Journal	Analytical Biochemistry
Volume	330
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2004.04.001
State	Published - 1 Jul 2004

Keywords

Absorbance
Aggregation
Hybridization
Ligand exchange
Luminescence
Oligonucleotide
Organic monolayer-protected silver nanoparticle
Surface-enhanced fluorescence
Tiopronin

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10.1016/j.ab.2004.04.001

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title = "Oligonucleotide-displaced organic monolayer-protected silver nanoparticles and enhanced luminescence of their salted aggregates",

abstract = "N-(2-mercaptopropionyl)glycine (tiopronin) monolayer-protected silver particles were partially displaced by single-stranded oligonucleotides through ligand exchanges. The oligonucleotide-displaced particles could be hybridized with complementary fluorophore-labeled oligonucleotides. Both the oligonucleotide-displaced and hybridized particles could be aggregated by electrostatic interactions with salt in buffer solution, and the aggregates displayed enhanced luminescence from fluorophores. This result suggests the possible application of surface-enhanced fluorescence from metallic nanoparticle aggregation for DNA detection.",

keywords = "Absorbance, Aggregation, Hybridization, Ligand exchange, Luminescence, Oligonucleotide, Organic monolayer-protected silver nanoparticle, Surface-enhanced fluorescence, Tiopronin",

author = "Jian Zhang and Joanna Malicka and Ignacy Gryczynski and Lakowicz, {Joseph R.}",

note = "Funding Information: This research was supported by a grant from NIH, NCRR, RR-08119, and HG02655 of NIHGRI.",

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AU - Zhang, Jian

AU - Malicka, Joanna

AU - Gryczynski, Ignacy

AU - Lakowicz, Joseph R.

N1 - Funding Information: This research was supported by a grant from NIH, NCRR, RR-08119, and HG02655 of NIHGRI.

PY - 2004/7/1

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N2 - N-(2-mercaptopropionyl)glycine (tiopronin) monolayer-protected silver particles were partially displaced by single-stranded oligonucleotides through ligand exchanges. The oligonucleotide-displaced particles could be hybridized with complementary fluorophore-labeled oligonucleotides. Both the oligonucleotide-displaced and hybridized particles could be aggregated by electrostatic interactions with salt in buffer solution, and the aggregates displayed enhanced luminescence from fluorophores. This result suggests the possible application of surface-enhanced fluorescence from metallic nanoparticle aggregation for DNA detection.

AB - N-(2-mercaptopropionyl)glycine (tiopronin) monolayer-protected silver particles were partially displaced by single-stranded oligonucleotides through ligand exchanges. The oligonucleotide-displaced particles could be hybridized with complementary fluorophore-labeled oligonucleotides. Both the oligonucleotide-displaced and hybridized particles could be aggregated by electrostatic interactions with salt in buffer solution, and the aggregates displayed enhanced luminescence from fluorophores. This result suggests the possible application of surface-enhanced fluorescence from metallic nanoparticle aggregation for DNA detection.

KW - Absorbance

KW - Aggregation

KW - Hybridization

KW - Ligand exchange

KW - Luminescence

KW - Oligonucleotide

KW - Organic monolayer-protected silver nanoparticle

KW - Surface-enhanced fluorescence

KW - Tiopronin

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DO - 10.1016/j.ab.2004.04.001

M3 - Article

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AN - SCOPUS:2942511808

SN - 0003-2697

VL - 330

SP - 81

EP - 86

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Oligonucleotide-displaced organic monolayer-protected silver nanoparticles and enhanced luminescence of their salted aggregates

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Keywords

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