Oligonucleotide-displaced organic monolayer-protected silver nanoparticles and enhanced luminescence of their salted aggregates

Jian Zhang, Joanna Malicka, Ignacy Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

N-(2-mercaptopropionyl)glycine (tiopronin) monolayer-protected silver particles were partially displaced by single-stranded oligonucleotides through ligand exchanges. The oligonucleotide-displaced particles could be hybridized with complementary fluorophore-labeled oligonucleotides. Both the oligonucleotide-displaced and hybridized particles could be aggregated by electrostatic interactions with salt in buffer solution, and the aggregates displayed enhanced luminescence from fluorophores. This result suggests the possible application of surface-enhanced fluorescence from metallic nanoparticle aggregation for DNA detection.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalAnalytical Biochemistry
Volume330
Issue number1
DOIs
StatePublished - 1 Jul 2004

Keywords

  • Absorbance
  • Aggregation
  • Hybridization
  • Ligand exchange
  • Luminescence
  • Oligonucleotide
  • Organic monolayer-protected silver nanoparticle
  • Surface-enhanced fluorescence
  • Tiopronin

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