Novel regulation of protein kinase C-η

Deepanwita Pal, Shalini Persaud Outram, Alakananda Basu

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Protein kinase C (PKC) is the receptor for tumor promoting phorbol esters, which are potent activators of conventional and novel PKCs, but persistent treatment with phorbol esters leads to downregulation of these PKCs. However, PKCη, a novel PKC isozyme, resists downregulation by tumor-promoting phorbol esters, but little is known about how PKCη level is regulated. Phosphorylation and dephosphorylation play an important role in regulating activity and stability of PKCs. In the present study, we have investigated the molecular mechanism of PKCη regulation. Several PKC activators, including phorbol 12,13-dibutyrate, 12-O-tetradecanoylphorbol-13-acetate and indolactam V caused upregulation of PKCη, whereas the general PKC inhibitor Gö 6983, but not the conventional PKC inhibitor Gö 6976 led to the downregulation of PKCη. Upregulation of PKCη was associated with an increase in phosphorylation of PKCη. Silencing of phosphoinositide-dependent kinase-1, which phosphorylates PKCη at the activation loop, failed to prevent PKC activator-induced upregulation of PKCη. Knockdown of PKCε but not PKCα inhibited PKC activator-induced upregulation of PKCη. Thus, our results suggest that the regulation of PKCη is unique and PKCε is required for the PKC activator-induced upregulation of PKCη.

Original languageEnglish
Pages (from-to)836-841
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume425
Issue number4
DOIs
StatePublished - 7 Sep 2012

Fingerprint

Protein Kinase C
Up-Regulation
Phorbol Esters
Down-Regulation
Phosphorylation
Protein C Inhibitor
Protein Kinase Inhibitors
Tumors
Phorbol 12,13-Dibutyrate
1-Phosphatidylinositol 4-Kinase
Tetradecanoylphorbol Acetate

Keywords

  • Phosphorylation
  • PKCε
  • PKCη
  • Tumor promoter
  • Upregulation

Cite this

Pal, Deepanwita ; Outram, Shalini Persaud ; Basu, Alakananda. / Novel regulation of protein kinase C-η. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 425, No. 4. pp. 836-841.
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Novel regulation of protein kinase C-η. / Pal, Deepanwita; Outram, Shalini Persaud; Basu, Alakananda.

In: Biochemical and Biophysical Research Communications, Vol. 425, No. 4, 07.09.2012, p. 836-841.

Research output: Contribution to journalArticleResearchpeer-review

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