Novel plasmonic platform for ultra-sensitive detection and diagnostics

Sangram Limbaji Raut, Ryan Rich, Tanya Shtoyko, Sebastian Requena, Ilkay Bora, Bo W. Laursen, Thomas Just Sørensen, Julian Borejdo, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

Abstract

In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA.Cl) in PVA films made from 0.2 % PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) was assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA.Cl in PVA film. In addition, we also tested the ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than 20-fold fluorescence enhancement (bright spots) accompanied by decrease in fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with and without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging IX
EditorsJorg Enderlein, Ingo Gregor, Zygmunt Karol Gryczynski, Rainer Erdmann, Felix Koberling
PublisherSPIE
Volume9714
ISBN (Electronic)9781628419481
DOIs
StatePublished - 1 Jan 2016
EventSingle Molecule Spectroscopy and Superresolution Imaging IX - San Francisco, United States
Duration: 13 Feb 201614 Feb 2016

Other

OtherSingle Molecule Spectroscopy and Superresolution Imaging IX
CountryUnited States
CitySan Francisco
Period13/02/1614/02/16

Fingerprint

platforms
Fluorescence
Fractals
fluorescence
Glass
fractals
Silver
Coloring Agents
Dyes
augmentation
dyes
Metals
silver
chutes
glass
Methyl Chloride
metals
methyl chloride
bioassay
Spin glass

Cite this

Raut, S. L., Rich, R., Shtoyko, T., Requena, S., Bora, I., Laursen, B. W., ... Gryczynski, I. (2016). Novel plasmonic platform for ultra-sensitive detection and diagnostics. In J. Enderlein, I. Gregor, Z. K. Gryczynski, R. Erdmann, & F. Koberling (Eds.), Single Molecule Spectroscopy and Superresolution Imaging IX (Vol. 9714). [971419] SPIE. https://doi.org/10.1117/12.2221923
Raut, Sangram Limbaji ; Rich, Ryan ; Shtoyko, Tanya ; Requena, Sebastian ; Bora, Ilkay ; Laursen, Bo W. ; Sørensen, Thomas Just ; Borejdo, Julian ; Gryczynski, Zygmunt ; Gryczynski, Ignacy. / Novel plasmonic platform for ultra-sensitive detection and diagnostics. Single Molecule Spectroscopy and Superresolution Imaging IX. editor / Jorg Enderlein ; Ingo Gregor ; Zygmunt Karol Gryczynski ; Rainer Erdmann ; Felix Koberling. Vol. 9714 SPIE, 2016.
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title = "Novel plasmonic platform for ultra-sensitive detection and diagnostics",
abstract = "In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA.Cl) in PVA films made from 0.2 {\%} PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) was assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA.Cl in PVA film. In addition, we also tested the ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than 20-fold fluorescence enhancement (bright spots) accompanied by decrease in fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with and without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.",
author = "Raut, {Sangram Limbaji} and Ryan Rich and Tanya Shtoyko and Sebastian Requena and Ilkay Bora and Laursen, {Bo W.} and S{\o}rensen, {Thomas Just} and Julian Borejdo and Zygmunt Gryczynski and Ignacy Gryczynski",
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Raut, SL, Rich, R, Shtoyko, T, Requena, S, Bora, I, Laursen, BW, Sørensen, TJ, Borejdo, J, Gryczynski, Z & Gryczynski, I 2016, Novel plasmonic platform for ultra-sensitive detection and diagnostics. in J Enderlein, I Gregor, ZK Gryczynski, R Erdmann & F Koberling (eds), Single Molecule Spectroscopy and Superresolution Imaging IX. vol. 9714, 971419, SPIE, Single Molecule Spectroscopy and Superresolution Imaging IX, San Francisco, United States, 13/02/16. https://doi.org/10.1117/12.2221923

Novel plasmonic platform for ultra-sensitive detection and diagnostics. / Raut, Sangram Limbaji; Rich, Ryan; Shtoyko, Tanya; Requena, Sebastian; Bora, Ilkay; Laursen, Bo W.; Sørensen, Thomas Just; Borejdo, Julian; Gryczynski, Zygmunt; Gryczynski, Ignacy.

Single Molecule Spectroscopy and Superresolution Imaging IX. ed. / Jorg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling. Vol. 9714 SPIE, 2016. 971419.

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

TY - GEN

T1 - Novel plasmonic platform for ultra-sensitive detection and diagnostics

AU - Raut, Sangram Limbaji

AU - Rich, Ryan

AU - Shtoyko, Tanya

AU - Requena, Sebastian

AU - Bora, Ilkay

AU - Laursen, Bo W.

AU - Sørensen, Thomas Just

AU - Borejdo, Julian

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

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N2 - In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA.Cl) in PVA films made from 0.2 % PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) was assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA.Cl in PVA film. In addition, we also tested the ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than 20-fold fluorescence enhancement (bright spots) accompanied by decrease in fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with and without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.

AB - In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA.Cl) in PVA films made from 0.2 % PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) was assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA.Cl in PVA film. In addition, we also tested the ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than 20-fold fluorescence enhancement (bright spots) accompanied by decrease in fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with and without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.

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U2 - 10.1117/12.2221923

DO - 10.1117/12.2221923

M3 - Conference contribution

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BT - Single Molecule Spectroscopy and Superresolution Imaging IX

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A2 - Gregor, Ingo

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PB - SPIE

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Raut SL, Rich R, Shtoyko T, Requena S, Bora I, Laursen BW et al. Novel plasmonic platform for ultra-sensitive detection and diagnostics. In Enderlein J, Gregor I, Gryczynski ZK, Erdmann R, Koberling F, editors, Single Molecule Spectroscopy and Superresolution Imaging IX. Vol. 9714. SPIE. 2016. 971419 https://doi.org/10.1117/12.2221923