Novel plasmonic platform for ultra-sensitive detection and diagnostics

Sangram Limbaji Raut; Ryan Rich; Tanya Shtoyko; Sebastian Requena; Ilkay Bora; Bo W. Laursen; Thomas Just Sørensen; Julian Borejdo; Zygmunt Gryczynski; Ignacy Gryczynski

doi:10.1117/12.2221923

Novel plasmonic platform for ultra-sensitive detection and diagnostics

Sangram Limbaji Raut, Ryan Rich, Tanya Shtoyko, Sebastian Requena, Ilkay Bora, Bo W. Laursen, Thomas Just Sørensen, Julian Borejdo, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA.Cl) in PVA films made from 0.2 % PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) was assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA.Cl in PVA film. In addition, we also tested the ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than 20-fold fluorescence enhancement (bright spots) accompanied by decrease in fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (G_A) and fluorescence radiative rate enhancements factor (G_F). No change in emission spectrum was observed for a dye with and without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.

Original language	English
Title of host publication	Single Molecule Spectroscopy and Superresolution Imaging IX
Editors	Jorg Enderlein, Ingo Gregor, Zygmunt Karol Gryczynski, Rainer Erdmann, Felix Koberling
Publisher	SPIE
Volume	9714
ISBN (Electronic)	9781628419481
DOIs	https://doi.org/10.1117/12.2221923
State	Published - 1 Jan 2016
Event	Single Molecule Spectroscopy and Superresolution Imaging IX - San Francisco, United States Duration: 13 Feb 2016 → 14 Feb 2016

Other

Other	Single Molecule Spectroscopy and Superresolution Imaging IX
Country/Territory	United States
City	San Francisco
Period	13/02/16 → 14/02/16

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10.1117/12.2221923

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Raut, S. L., Rich, R., Shtoyko, T., Requena, S., Bora, I., Laursen, B. W., Sørensen, T. J., Borejdo, J., Gryczynski, Z., & Gryczynski, I. (2016). Novel plasmonic platform for ultra-sensitive detection and diagnostics. In J. Enderlein, I. Gregor, Z. K. Gryczynski, R. Erdmann, & F. Koberling (Eds.), Single Molecule Spectroscopy and Superresolution Imaging IX (Vol. 9714). [971419] SPIE. https://doi.org/10.1117/12.2221923

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title = "Novel plasmonic platform for ultra-sensitive detection and diagnostics",

abstract = "In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA.Cl) in PVA films made from 0.2 % PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) was assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA.Cl in PVA film. In addition, we also tested the ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than 20-fold fluorescence enhancement (bright spots) accompanied by decrease in fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with and without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.",

author = "Raut, {Sangram Limbaji} and Ryan Rich and Tanya Shtoyko and Sebastian Requena and Ilkay Bora and Laursen, {Bo W.} and S{\o}rensen, {Thomas Just} and Julian Borejdo and Zygmunt Gryczynski and Ignacy Gryczynski",

year = "2016",

month = jan,

day = "1",

doi = "10.1117/12.2221923",

language = "English",

volume = "9714",

editor = "Jorg Enderlein and Ingo Gregor and Gryczynski, {Zygmunt Karol} and Rainer Erdmann and Felix Koberling",

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publisher = "SPIE",

note = "Single Molecule Spectroscopy and Superresolution Imaging IX ; Conference date: 13-02-2016 Through 14-02-2016",

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Raut, SL, Rich, R, Shtoyko, T, Requena, S, Bora, I, Laursen, BW, Sørensen, TJ, Borejdo, J, Gryczynski, Z & Gryczynski, I 2016, Novel plasmonic platform for ultra-sensitive detection and diagnostics. in J Enderlein, I Gregor, ZK Gryczynski, R Erdmann & F Koberling (eds), Single Molecule Spectroscopy and Superresolution Imaging IX. vol. 9714, 971419, SPIE, Single Molecule Spectroscopy and Superresolution Imaging IX, San Francisco, United States, 13/02/16. https://doi.org/10.1117/12.2221923

Novel plasmonic platform for ultra-sensitive detection and diagnostics. / Raut, Sangram Limbaji; Rich, Ryan; Shtoyko, Tanya et al.
Single Molecule Spectroscopy and Superresolution Imaging IX. ed. / Jorg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling. Vol. 9714 SPIE, 2016. 971419.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Novel plasmonic platform for ultra-sensitive detection and diagnostics

AU - Raut, Sangram Limbaji

AU - Rich, Ryan

AU - Shtoyko, Tanya

AU - Requena, Sebastian

AU - Bora, Ilkay

AU - Laursen, Bo W.

AU - Sørensen, Thomas Just

AU - Borejdo, Julian

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

PY - 2016/1/1

Y1 - 2016/1/1

N2 - In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA.Cl) in PVA films made from 0.2 % PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) was assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA.Cl in PVA film. In addition, we also tested the ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than 20-fold fluorescence enhancement (bright spots) accompanied by decrease in fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with and without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.

AB - In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA.Cl) in PVA films made from 0.2 % PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) was assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA.Cl in PVA film. In addition, we also tested the ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than 20-fold fluorescence enhancement (bright spots) accompanied by decrease in fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with and without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.

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DO - 10.1117/12.2221923

M3 - Conference contribution

AN - SCOPUS:84983315369

VL - 9714

BT - Single Molecule Spectroscopy and Superresolution Imaging IX

A2 - Enderlein, Jorg

A2 - Gregor, Ingo

A2 - Gryczynski, Zygmunt Karol

A2 - Erdmann, Rainer

A2 - Koberling, Felix

PB - SPIE

T2 - Single Molecule Spectroscopy and Superresolution Imaging IX

Y2 - 13 February 2016 through 14 February 2016

ER -

Novel plasmonic platform for ultra-sensitive detection and diagnostics

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