Novel methods for fluorescence sensing

Joseph R. Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski, Jonathan D. Dattelbaum, Leah Tolosa, Govind Rao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Scopus citations

Abstract

We describe a new method for fluorescence sensing based on measurements of the steady state polarization of an analyte-sensitive fluorophore in the presence of a reference fluorophore with known polarization. The basic concept is that the polarization of a mixture reflects a weighted average of the polarization of the emitting species. By use of reference fluorophores the starting values can be near zero, or near 0.9 for oriented films which contain the reference fluorophore. Changing intensities of the sensing fluorophore due to the analyte result in changes in the polarization of the combined emission. A wide dynamic range is available because of the freedom to select high or low starting polarization values. Polarization-based sensing was demonstrated for pH using 6-carboxy fluorescein. We also show that polarization sensing can be used for measurements of oxygen and glucose. Polarization sensing can have numerous applications in clinical and analytical chemistry.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages234-243
Number of pages10
ISBN (Print)0819430722
StatePublished - 1 Jan 1999
EventProceedings of the 1999 Advances in Fluorescence Sensing Technology - San Jose, CA, USA
Duration: 24 Jan 199927 Jan 1999

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume3602
ISSN (Print)0277-786X

Other

OtherProceedings of the 1999 Advances in Fluorescence Sensing Technology
CitySan Jose, CA, USA
Period24/01/9927/01/99

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Lakowicz, J. R., Gryczynski, I., Gryczynski, Z., Dattelbaum, J. D., Tolosa, L., & Rao, G. (1999). Novel methods for fluorescence sensing. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 234-243). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 3602). Society of Photo-Optical Instrumentation Engineers.