Notch4 is activated in endothelial and smooth muscle cells in human brain arteriovenous malformations

Qichuan Zhuge, Zhebao Wu, Lijie Huang, Bei Zhao, Ming Zhong, Weiming Zheng, Chen Gourong, Xiaoou Mao, Lin Xie, Xiangdong Wang, Kunlin Jin

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Up-regulation of Notch4 was observed in the endothelial cells in the arteriovenous malformations (AVMs) in mice. However, whether Notch4 is also involved in brain AVMs in humans remains unclear. Here, we performed immunohistochemistry on normal brain vascular tissue and surgically resected brain AVMs and found that Notch4 was up-regulated in the subset of abnormal vessels of the brain AVM nidus, compared with control brain vascular tissue. Two-photon confocal images show that Notch4 was expressed not only in the endothelial but also in the smooth muscle cells of the vascular wall in brain AVMs. Western blotting shows that Notch4 was activated in brain AVMs, but not in middle cerebral artery of normal human brain, which was confirmed by immunostaining. Our findings suggest a possible contribution of Notch4 signalling to the development of brain AVMs in human.

Original languageEnglish
Pages (from-to)1458-1464
Number of pages7
JournalJournal of Cellular and Molecular Medicine
Volume17
Issue number11
DOIs
StatePublished - Nov 2013

Keywords

  • AVM
  • Brain
  • Human
  • Notch4
  • Signalling

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    Zhuge, Q., Wu, Z., Huang, L., Zhao, B., Zhong, M., Zheng, W., Gourong, C., Mao, X., Xie, L., Wang, X., & Jin, K. (2013). Notch4 is activated in endothelial and smooth muscle cells in human brain arteriovenous malformations. Journal of Cellular and Molecular Medicine, 17(11), 1458-1464. https://doi.org/10.1111/jcmm.12115