Time-resolved fluorescence spectroscopy and recently time-resolved fluorescence microscopy have proven to be powerful technologies for studying macromolecular interactions and dynamics in biological systems on the subpicosecond to millisecond timescale. In complex biological systems including membranes, nuclei, or even entire cells, the fluorescence decay kinetics can reveal detailed information about intrinsic relaxation mechanisms, macromolecular interactions, conformational changes, and dynamics of complex molecular processes. Depending on the excitation type, the instrumental methods for measuring fluorescence intensity decays (fluorescence lifetimes) traditionally are divided into two dominant methods: time domain (O’Connor and Philips 1984; Birch and Imhof 1991; Demas 1983; Chapter 6) and frequency domain (Gratton and Limkeman 1983; Gratton et al. 1984; Lakowicz 1999; Chapter 5).
|Title of host publication||Flim Microscopy in Biology and Medicine|
|Number of pages||29|
|State||Published - 1 Jan 2009|