TY - JOUR
T1 - Non-selenium-dependent glutathione peroxidase activity in rat lung
T2 - Association with lung glutathione S-transferase activity and the effects of hyperoxia
AU - Jenkinson, Stephen G.
AU - Lawrence, Richard A.
AU - Burk, Raymond F.
AU - Gregory, Paula E.
N1 - Funding Information:
’ Supported by NIH Grant HL 266 18 and ES 02497.
PY - 1983/5
Y1 - 1983/5
N2 - To determine if non-selenium-dependent glutathione peroxidase (Non-Se GSH-Px) activity is present in rat lung, we fractionated rat lung soluble fractions from rats fed a selenium-deficient or control diet and measured glutathione peroxidase activity with both cumene hydroperoxide and hydrogen peroxide as substrates. We also measured glutathione S-transferase (GSH S-transferase) activity in the fractions with 1-chloro-2,4-dinitrobenzene as substrate. Non-Se GSH-Px activity was present (about 34% of total GSH-Px activity), and the peak present in the gel filtration chromatogram coeluted with the GSH S-transferase peak. We then measured GSH S-transferase activity in lung-soluble fractions from rats exposed to room air or 85% O2 for 5 days. Lung GSH S-transferase activity was increased in the oxygen-exposed animals when compared to the air-exposed controls. The increase in GSH S-transferase activity could represent the induction of lung non-Se GSH-Px activity.
AB - To determine if non-selenium-dependent glutathione peroxidase (Non-Se GSH-Px) activity is present in rat lung, we fractionated rat lung soluble fractions from rats fed a selenium-deficient or control diet and measured glutathione peroxidase activity with both cumene hydroperoxide and hydrogen peroxide as substrates. We also measured glutathione S-transferase (GSH S-transferase) activity in the fractions with 1-chloro-2,4-dinitrobenzene as substrate. Non-Se GSH-Px activity was present (about 34% of total GSH-Px activity), and the peak present in the gel filtration chromatogram coeluted with the GSH S-transferase peak. We then measured GSH S-transferase activity in lung-soluble fractions from rats exposed to room air or 85% O2 for 5 days. Lung GSH S-transferase activity was increased in the oxygen-exposed animals when compared to the air-exposed controls. The increase in GSH S-transferase activity could represent the induction of lung non-Se GSH-Px activity.
UR - http://www.scopus.com/inward/record.url?scp=0020628683&partnerID=8YFLogxK
U2 - 10.1016/0041-008X(83)90284-3
DO - 10.1016/0041-008X(83)90284-3
M3 - Article
C2 - 6857674
AN - SCOPUS:0020628683
VL - 68
SP - 399
EP - 404
JO - Toxicology and Applied Pharmacology
JF - Toxicology and Applied Pharmacology
SN - 0041-008X
IS - 3
ER -