Abstract
Interlaboratory studies are a type of collaborative exercise in which many laboratories are presented with the same set of data to interpret, and the results they produce are examined to get a “big picture” view of the effectiveness and accuracy of analytical protocols used across participating laboratories. In 2005 and again in 2013, the Applied Genetics Group of the National Institute of Standards and Technology (NIST) conducted interlaboratory studies involving DNA mixture interpretation. In the 2005 NIST MIX05 study, 69 laboratories interpreted data in the form of electropherograms of two-person DNA mixtures representing four different mock sexual assault cases with different contributor ratios. In the 2013 NIST MIX13 study,108 laboratories interpreted electropherogram data for five different case scenarios involving two, three, or four contributors, with some of the contributors potentially related. This paper describes the design of these studies, the variations observed among laboratory results, and lessons learned.
| Original language | English |
|---|---|
| Pages (from-to) | 81-94 |
| Number of pages | 14 |
| Journal | Forensic Science International: Genetics |
| Volume | 37 |
| DOIs | |
| State | Published - 1 Nov 2018 |
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Keywords
- Collaborative exercise
- DNA mixture
- Forensic DNA
- Forensic science
- Interlaboratory study
- MIX05
- MIX13
- Mixture interpretation
Cite this
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NIST interlaboratory studies involving DNA mixtures (MIX05 and MIX13) : Variation observed and lessons learned. / Butler, John M.; Kline, Margaret C.; Coble, Michael Dewitt.
In: Forensic Science International: Genetics, Vol. 37, 01.11.2018, p. 81-94.Research output: Contribution to journal › Article
TY - JOUR
T1 - NIST interlaboratory studies involving DNA mixtures (MIX05 and MIX13)
T2 - Variation observed and lessons learned
AU - Butler, John M.
AU - Kline, Margaret C.
AU - Coble, Michael Dewitt
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Interlaboratory studies are a type of collaborative exercise in which many laboratories are presented with the same set of data to interpret, and the results they produce are examined to get a “big picture” view of the effectiveness and accuracy of analytical protocols used across participating laboratories. In 2005 and again in 2013, the Applied Genetics Group of the National Institute of Standards and Technology (NIST) conducted interlaboratory studies involving DNA mixture interpretation. In the 2005 NIST MIX05 study, 69 laboratories interpreted data in the form of electropherograms of two-person DNA mixtures representing four different mock sexual assault cases with different contributor ratios. In the 2013 NIST MIX13 study,108 laboratories interpreted electropherogram data for five different case scenarios involving two, three, or four contributors, with some of the contributors potentially related. This paper describes the design of these studies, the variations observed among laboratory results, and lessons learned.
AB - Interlaboratory studies are a type of collaborative exercise in which many laboratories are presented with the same set of data to interpret, and the results they produce are examined to get a “big picture” view of the effectiveness and accuracy of analytical protocols used across participating laboratories. In 2005 and again in 2013, the Applied Genetics Group of the National Institute of Standards and Technology (NIST) conducted interlaboratory studies involving DNA mixture interpretation. In the 2005 NIST MIX05 study, 69 laboratories interpreted data in the form of electropherograms of two-person DNA mixtures representing four different mock sexual assault cases with different contributor ratios. In the 2013 NIST MIX13 study,108 laboratories interpreted electropherogram data for five different case scenarios involving two, three, or four contributors, with some of the contributors potentially related. This paper describes the design of these studies, the variations observed among laboratory results, and lessons learned.
KW - Collaborative exercise
KW - DNA mixture
KW - Forensic DNA
KW - Forensic science
KW - Interlaboratory study
KW - MIX05
KW - MIX13
KW - Mixture interpretation
UR - http://www.scopus.com/inward/record.url?scp=85051394966&partnerID=8YFLogxK
U2 - 10.1016/j.fsigen.2018.07.024
DO - 10.1016/j.fsigen.2018.07.024
M3 - Article
C2 - 30103146
AN - SCOPUS:85051394966
VL - 37
SP - 81
EP - 94
JO - Forensic Science International: Genetics
JF - Forensic Science International: Genetics
SN - 1872-4973
ER -