Abstract
The structures of the O-polysaccharides of the lipopolysaccharides of Proteus mirabilis O7 and O49 were determined by chemical methods, mass spectrometry, including MS/MS, and NMR spectroscopy, including experiments run in an H2O/D2O mixture to reveal correlations for NH protons. The O-polysaccharides were found to contain N-carboxyacetyl (malonyl) and N-(3-carboxypropanoyl) (succinyl) derivatives of 4-amino-4,6-dideoxyglucose (4-amino-4-deoxyquinovose, Qui4N), respectively. The behavior of Qui4N derivatives with the dicarboxylic acids under conditions of acid hydrolysis and methanolysis was studied using GLC-MS.
Original language | English |
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Pages (from-to) | 1034-1043 |
Number of pages | 10 |
Journal | Biochemistry (Moscow) |
Volume | 69 |
Issue number | 9 |
DOIs | |
State | Published - Sep 2004 |
Keywords
- 4-amino-4,6-dideoxy-d-glucose
- Aspartic acid
- MS/MS
- Malonic acid
- O-polysaccharide
- Proteus mirabilis
- Succinic acid