New structures of the O-specific polysaccharides of Proteus. 4. Polysaccharides containing unusual acidic N-acyl derivatives of 4-amino-4,6-dideoxy-D-glucose

A. N. Kondakova, B. Linder, Rafal Fudala, S. N. Senchenkova, H. Moll, A. S. Shashkov, W. Kaca, U. Zähringer, Y. A. Knirel

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The structures of the O-polysaccharides of the lipopolysaccharides of Proteus mirabilis O7 and O49 were determined by chemical methods, mass spectrometry, including MS/MS, and NMR spectroscopy, including experiments run in an H2O/D2O mixture to reveal correlations for NH protons. The O-polysaccharides were found to contain N-carboxyacetyl (malonyl) and N-(3-carboxypropanoyl) (succinyl) derivatives of 4-amino-4,6-dideoxyglucose (4-amino-4-deoxyquinovose, Qui4N), respectively. The behavior of Qui4N derivatives with the dicarboxylic acids under conditions of acid hydrolysis and methanolysis was studied using GLC-MS.

Original languageEnglish
Pages (from-to)1034-1043
Number of pages10
JournalBiochemistry (Moscow)
Volume69
Issue number9
DOIs
StatePublished - 1 Sep 2004

Keywords

  • 4-amino-4,6-dideoxy-d-glucose
  • Aspartic acid
  • MS/MS
  • Malonic acid
  • O-polysaccharide
  • Proteus mirabilis
  • Succinic acid

Fingerprint Dive into the research topics of 'New structures of the O-specific polysaccharides of Proteus. 4. Polysaccharides containing unusual acidic N-acyl derivatives of 4-amino-4,6-dideoxy-D-glucose'. Together they form a unique fingerprint.

  • Cite this