New structures of the O-specific polysaccharides of Proteus. 4. Polysaccharides containing unusual acidic N-acyl derivatives of 4-amino-4,6-dideoxy-D-glucose

A. N. Kondakova; B. Linder; R. Fudala; S. N. Senchenkova; H. Moll; A. S. Shashkov; W. Kaca; U. Zahringer; Y. A. Knirel

New structures of the O-specific polysaccharides of Proteus. 4. Polysaccharides containing unusual acidic N-acyl derivatives of 4-amino-4,6-dideoxy-D-glucose

A. N. Kondakova, B. Linder, R. Fudala, S. N. Senchenkova, H. Moll, A. S. Shashkov, W. Kaca, U. Zahringer, Y. A. Knirel

Research output: Contribution to journal › Article › peer-review

Abstract

The structures of the O-polysaccharides of the lipopolysaccharides of Proteus mirabilis O7 and O49 were determined by chemical methods, mass spectrometry, including MS/MS, and NMR spectroscopy, including experiments run in an H₂O/D₂O mixture to reveal correlations for NH protons. The O-polysaccharides were found to contain N-carboxyacetyl (malonyl) and N-(3-carboxypropanoyl) (succinyl) derivatives of 4-amino-4,6-dideoxyglucose (4-amino-4-deoxyquinovose, Qui4N), respectively. The behavior of Qui4N derivatives with the dicarboxylic acids under conditions of acid hydrolysis and methanolysis was studied using GLC-MS.

Original language	English
Pages (from-to)	1271-1282
Number of pages	12
Journal	Biokhimiya
Volume	69
Issue number	9
State	Published - 21 Dec 2004

Keywords

4-amino-4,6-dideoxy-D-glucose
Aspartic acid
MS/MS
Malonic acid
O-polysaccharide
Proteus mirabilis
Succinic acid

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title = "New structures of the O-specific polysaccharides of Proteus. 4. Polysaccharides containing unusual acidic N-acyl derivatives of 4-amino-4,6-dideoxy-D-glucose",

abstract = "The structures of the O-polysaccharides of the lipopolysaccharides of Proteus mirabilis O7 and O49 were determined by chemical methods, mass spectrometry, including MS/MS, and NMR spectroscopy, including experiments run in an H2O/D2O mixture to reveal correlations for NH protons. The O-polysaccharides were found to contain N-carboxyacetyl (malonyl) and N-(3-carboxypropanoyl) (succinyl) derivatives of 4-amino-4,6-dideoxyglucose (4-amino-4-deoxyquinovose, Qui4N), respectively. The behavior of Qui4N derivatives with the dicarboxylic acids under conditions of acid hydrolysis and methanolysis was studied using GLC-MS.",

keywords = "4-amino-4,6-dideoxy-D-glucose, Aspartic acid, MS/MS, Malonic acid, O-polysaccharide, Proteus mirabilis, Succinic acid",

author = "Kondakova, {A. N.} and B. Linder and R. Fudala and Senchenkova, {S. N.} and H. Moll and Shashkov, {A. S.} and W. Kaca and U. Zahringer and Knirel, {Y. A.}",

year = "2004",

month = dec,

day = "21",

language = "English",

volume = "69",

pages = "1271--1282",

journal = "Biokhimiya",

issn = "0320-9725",

publisher = "Izdatel'stvo Nauka",

number = "9",

}

TY - JOUR

T1 - New structures of the O-specific polysaccharides of Proteus. 4. Polysaccharides containing unusual acidic N-acyl derivatives of 4-amino-4,6-dideoxy-D-glucose

AU - Kondakova, A. N.

AU - Linder, B.

AU - Fudala, R.

AU - Senchenkova, S. N.

AU - Moll, H.

AU - Shashkov, A. S.

AU - Kaca, W.

AU - Zahringer, U.

AU - Knirel, Y. A.

PY - 2004/12/21

Y1 - 2004/12/21

N2 - The structures of the O-polysaccharides of the lipopolysaccharides of Proteus mirabilis O7 and O49 were determined by chemical methods, mass spectrometry, including MS/MS, and NMR spectroscopy, including experiments run in an H2O/D2O mixture to reveal correlations for NH protons. The O-polysaccharides were found to contain N-carboxyacetyl (malonyl) and N-(3-carboxypropanoyl) (succinyl) derivatives of 4-amino-4,6-dideoxyglucose (4-amino-4-deoxyquinovose, Qui4N), respectively. The behavior of Qui4N derivatives with the dicarboxylic acids under conditions of acid hydrolysis and methanolysis was studied using GLC-MS.

AB - The structures of the O-polysaccharides of the lipopolysaccharides of Proteus mirabilis O7 and O49 were determined by chemical methods, mass spectrometry, including MS/MS, and NMR spectroscopy, including experiments run in an H2O/D2O mixture to reveal correlations for NH protons. The O-polysaccharides were found to contain N-carboxyacetyl (malonyl) and N-(3-carboxypropanoyl) (succinyl) derivatives of 4-amino-4,6-dideoxyglucose (4-amino-4-deoxyquinovose, Qui4N), respectively. The behavior of Qui4N derivatives with the dicarboxylic acids under conditions of acid hydrolysis and methanolysis was studied using GLC-MS.

KW - 4-amino-4,6-dideoxy-D-glucose

KW - Aspartic acid

KW - MS/MS

KW - Malonic acid

KW - O-polysaccharide

KW - Proteus mirabilis

KW - Succinic acid

UR - http://www.scopus.com/inward/record.url?scp=10144222905&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:10144222905

SN - 0320-9725

VL - 69

SP - 1271

EP - 1282

JO - Biokhimiya

JF - Biokhimiya

IS - 9

ER -

New structures of the O-specific polysaccharides of Proteus. 4. Polysaccharides containing unusual acidic N-acyl derivatives of 4-amino-4,6-dideoxy-D-glucose

Abstract

Keywords

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