New structures of the O-specific polysaccharides of Proteus. 4. Polysaccharides containing unusual acidic N-acyl derivatives of 4-amino-4,6-dideoxy-D-glucose

A. N. Kondakova, B. Linder, R. Fudala, S. N. Senchenkova, H. Moll, A. S. Shashkov, W. Kaca, U. Zahringer, Y. A. Knirel

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Abstract

The structures of the O-polysaccharides of the lipopolysaccharides of Proteus mirabilis O7 and O49 were determined by chemical methods, mass spectrometry, including MS/MS, and NMR spectroscopy, including experiments run in an H2O/D2O mixture to reveal correlations for NH protons. The O-polysaccharides were found to contain N-carboxyacetyl (malonyl) and N-(3-carboxypropanoyl) (succinyl) derivatives of 4-amino-4,6-dideoxyglucose (4-amino-4-deoxyquinovose, Qui4N), respectively. The behavior of Qui4N derivatives with the dicarboxylic acids under conditions of acid hydrolysis and methanolysis was studied using GLC-MS.

Original languageEnglish
Pages (from-to)1271-1282
Number of pages12
JournalBiokhimiya
Volume69
Issue number9
StatePublished - 21 Dec 2004

Keywords

  • 4-amino-4,6-dideoxy-D-glucose
  • Aspartic acid
  • MS/MS
  • Malonic acid
  • O-polysaccharide
  • Proteus mirabilis
  • Succinic acid

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    Kondakova, A. N., Linder, B., Fudala, R., Senchenkova, S. N., Moll, H., Shashkov, A. S., Kaca, W., Zahringer, U., & Knirel, Y. A. (2004). New structures of the O-specific polysaccharides of Proteus. 4. Polysaccharides containing unusual acidic N-acyl derivatives of 4-amino-4,6-dideoxy-D-glucose. Biokhimiya, 69(9), 1271-1282.