TY - JOUR
T1 - Neutrophils Are More Effective than Monocytes at Phagosomal Containment and Killing of Listeria monocytogenes
AU - Okunnu, Busola M.
AU - Berg, Rance E.
N1 - Funding Information:
Received for publication August 22, 2019. Accepted for publication November 22, 2019. Address correspondence and reprint requests to: Dr. Rance E. Berg, Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107. E-mail address: Rance.Berg@unthsc.edu ORCIDs: 0000-0001-6720-0443 (B.M.O.); 0000-0002-2934-382X (R.E.B.). This work was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award R25GM125587. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Abbreviations used in this article: BHI, brain heart infusion; H2DCFDA, 2979-dichlorodihydrofluorescein diacetate; HKLM, heat-killed L. monocytogenes; iNOS, inducible NO synthase; LAMP-1, lysosome associated membrane protein-1; LLO, Listeriolysin O; MFI, mean fluorescence intensity; MOI, multiplicity of infection; ROS, reactive oxygen species. The online version of this article contains supplemental material. This article is distributed under the terms of the CC BY 4.0 Unported license. Copyright © 2019 The Authors
Publisher Copyright:
Copyright © 2019 The Authors
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Neutrophils and inflammatory monocytes are innate immune cells essential for protection during Listeria monocytogenes infection. Although certain functions have been generally assigned to each of the cells, similarities and differences in functions necessary for bacterial clearance have not previously been investigated. In the current study, phagocytosis, phagosomal containment, bacterial killing, and cytokine production by neutrophils and monocytes during L. monocytogenes infection were studied. Data obtained via in vitro studies show that neutrophils are more effective at L. monocytogenes uptake, phagosomal containment, and killing than monocytes. However, monocytes were found to be more effective at cytokine production during L. monocytogenes infection, in vivo. Additionally, the data demonstrated that neutrophils and monocytes are also capable of producing IL-1α, a cytokine that does not yet have a clearly defined role during infection with L. monocytogenes. Furthermore, we were able to demonstrate a population of monocytes producing both TNF-α and IL-α, concurrently. This study highlights the multifunctional capabilities of neutrophils and monocytes, further adding to our knowledge of these innate immune cells during L. monocytogenes infection.
AB - Neutrophils and inflammatory monocytes are innate immune cells essential for protection during Listeria monocytogenes infection. Although certain functions have been generally assigned to each of the cells, similarities and differences in functions necessary for bacterial clearance have not previously been investigated. In the current study, phagocytosis, phagosomal containment, bacterial killing, and cytokine production by neutrophils and monocytes during L. monocytogenes infection were studied. Data obtained via in vitro studies show that neutrophils are more effective at L. monocytogenes uptake, phagosomal containment, and killing than monocytes. However, monocytes were found to be more effective at cytokine production during L. monocytogenes infection, in vivo. Additionally, the data demonstrated that neutrophils and monocytes are also capable of producing IL-1α, a cytokine that does not yet have a clearly defined role during infection with L. monocytogenes. Furthermore, we were able to demonstrate a population of monocytes producing both TNF-α and IL-α, concurrently. This study highlights the multifunctional capabilities of neutrophils and monocytes, further adding to our knowledge of these innate immune cells during L. monocytogenes infection.
UR - http://www.scopus.com/inward/record.url?scp=85101190799&partnerID=8YFLogxK
U2 - 10.4049/immunohorizons.1900065
DO - 10.4049/immunohorizons.1900065
M3 - Article
C2 - 31836639
AN - SCOPUS:85101190799
SN - 2573-7732
VL - 3
SP - 573
EP - 584
JO - ImmunoHorizons
JF - ImmunoHorizons
IS - 12
ER -