Abstract
Orientin is a flavonoid monomer. In recent years, its importance as a source of pharmacological active substance is growing rapidly due to its properties such as anti-myocardial ischemia, anti-Apoptosis, anti-radiation, anti-Tumor, and anti-Aging. However, the neuroprotective effects of Orientin on stroke injury have not been comprehensively evaluated. The aim of the present study was thus to investigate the neuroprotective capacity and the potential mechanisms of Cyperus esculentus L. orientin (CLO) from Cyperus esculentus L. leaves against ischemia/reperfusion (I/R) injury using standard orientin as control. For in vitro studies, we treated HT22 cells with CoCl2 as an in vitro ischemic injury model. HT22 cells in the control group were treated with CoCl2. For in vivo studies, we used rat models of middle cerebral artery occlusion, and animals that received sham surgery were used as controls. We found that CLO protected CoCl2-induced HT22 cells against ischemia/reperfusion injury by lowering lipid peroxidation and reactive oxygen species formation as well as decreasing protein oxidation. However, CLO did not reduce the release of lactate dehydrogenase nor increase the activity of superoxide dismutase. Results showed that CLO could decrease neurological deficit score, attenuate brain water content, and reduce cerebral infarct volume, leading to neuroprotection during cerebral ischemia-reperfusion injury. Our studies indicate that CLO flavonoids can be taken as a natural antioxidant and bacteriostastic substance in food and pharmaceutical industry. The molecular mechanisms of CLO could be at least partially attributed to the antioxidant properties and subsequently inhibiting activation of casepase-3. All experimental procedures and protocols were approved on May 16, 2016 by the Experimental Animal Ethics Committee of Xinjiang Medical University of China (approval No. IACUC20160516-57).
| Original language | English |
|---|---|
| Pages (from-to) | 548-556 |
| Number of pages | 9 |
| Journal | Neural Regeneration Research |
| Volume | 15 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1 Mar 2020 |
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Keywords
- Antioxidants
- Caspase-3
- Cerebral ischemia/reperfusion injury
- Cobalt chloride
- Cyperus esculentus L. orientin (CLO)
- Lipid peroxidation
- Nerve regeneration
- Neurological deficits
- Oxidative stress
- Reactive oxygen species
Cite this
}
Neuroprotection of Cyperus esculentus L. orientin against cerebral ischemia/reperfusion induced brain injury. / Jing, Si Qun; Wang, Sai Sai; Zhong, Rui Min; Zhang, Jun Yan; Wu, Jin Zi; Tu, Yi Xian; Pu, Yan; Yan, Liang Jun.
In: Neural Regeneration Research, Vol. 15, No. 3, 01.03.2020, p. 548-556.Research output: Contribution to journal › Article
TY - JOUR
T1 - Neuroprotection of Cyperus esculentus L. orientin against cerebral ischemia/reperfusion induced brain injury
AU - Jing, Si Qun
AU - Wang, Sai Sai
AU - Zhong, Rui Min
AU - Zhang, Jun Yan
AU - Wu, Jin Zi
AU - Tu, Yi Xian
AU - Pu, Yan
AU - Yan, Liang Jun
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Orientin is a flavonoid monomer. In recent years, its importance as a source of pharmacological active substance is growing rapidly due to its properties such as anti-myocardial ischemia, anti-Apoptosis, anti-radiation, anti-Tumor, and anti-Aging. However, the neuroprotective effects of Orientin on stroke injury have not been comprehensively evaluated. The aim of the present study was thus to investigate the neuroprotective capacity and the potential mechanisms of Cyperus esculentus L. orientin (CLO) from Cyperus esculentus L. leaves against ischemia/reperfusion (I/R) injury using standard orientin as control. For in vitro studies, we treated HT22 cells with CoCl2 as an in vitro ischemic injury model. HT22 cells in the control group were treated with CoCl2. For in vivo studies, we used rat models of middle cerebral artery occlusion, and animals that received sham surgery were used as controls. We found that CLO protected CoCl2-induced HT22 cells against ischemia/reperfusion injury by lowering lipid peroxidation and reactive oxygen species formation as well as decreasing protein oxidation. However, CLO did not reduce the release of lactate dehydrogenase nor increase the activity of superoxide dismutase. Results showed that CLO could decrease neurological deficit score, attenuate brain water content, and reduce cerebral infarct volume, leading to neuroprotection during cerebral ischemia-reperfusion injury. Our studies indicate that CLO flavonoids can be taken as a natural antioxidant and bacteriostastic substance in food and pharmaceutical industry. The molecular mechanisms of CLO could be at least partially attributed to the antioxidant properties and subsequently inhibiting activation of casepase-3. All experimental procedures and protocols were approved on May 16, 2016 by the Experimental Animal Ethics Committee of Xinjiang Medical University of China (approval No. IACUC20160516-57).
AB - Orientin is a flavonoid monomer. In recent years, its importance as a source of pharmacological active substance is growing rapidly due to its properties such as anti-myocardial ischemia, anti-Apoptosis, anti-radiation, anti-Tumor, and anti-Aging. However, the neuroprotective effects of Orientin on stroke injury have not been comprehensively evaluated. The aim of the present study was thus to investigate the neuroprotective capacity and the potential mechanisms of Cyperus esculentus L. orientin (CLO) from Cyperus esculentus L. leaves against ischemia/reperfusion (I/R) injury using standard orientin as control. For in vitro studies, we treated HT22 cells with CoCl2 as an in vitro ischemic injury model. HT22 cells in the control group were treated with CoCl2. For in vivo studies, we used rat models of middle cerebral artery occlusion, and animals that received sham surgery were used as controls. We found that CLO protected CoCl2-induced HT22 cells against ischemia/reperfusion injury by lowering lipid peroxidation and reactive oxygen species formation as well as decreasing protein oxidation. However, CLO did not reduce the release of lactate dehydrogenase nor increase the activity of superoxide dismutase. Results showed that CLO could decrease neurological deficit score, attenuate brain water content, and reduce cerebral infarct volume, leading to neuroprotection during cerebral ischemia-reperfusion injury. Our studies indicate that CLO flavonoids can be taken as a natural antioxidant and bacteriostastic substance in food and pharmaceutical industry. The molecular mechanisms of CLO could be at least partially attributed to the antioxidant properties and subsequently inhibiting activation of casepase-3. All experimental procedures and protocols were approved on May 16, 2016 by the Experimental Animal Ethics Committee of Xinjiang Medical University of China (approval No. IACUC20160516-57).
KW - Antioxidants
KW - Caspase-3
KW - Cerebral ischemia/reperfusion injury
KW - Cobalt chloride
KW - Cyperus esculentus L. orientin (CLO)
KW - Lipid peroxidation
KW - Nerve regeneration
KW - Neurological deficits
KW - Oxidative stress
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85073003145&partnerID=8YFLogxK
U2 - 10.4103/1673-5374.266063
DO - 10.4103/1673-5374.266063
M3 - Article
AN - SCOPUS:85073003145
VL - 15
SP - 548
EP - 556
JO - Neural Regeneration Research
JF - Neural Regeneration Research
SN - 1673-5374
IS - 3
ER -