Neuroprotection of Cyperus esculentus L. orientin against cerebral ischemia/reperfusion induced brain injury

Si Qun Jing, Sai Sai Wang, Rui Min Zhong, Jun Yan Zhang, Jin Zi Wu, Yi Xian Tu, Yan Pu, Liang Jun Yan

Research output: Contribution to journalArticle

Abstract

Orientin is a flavonoid monomer. In recent years, its importance as a source of pharmacological active substance is growing rapidly due to its properties such as anti-myocardial ischemia, anti-Apoptosis, anti-radiation, anti-Tumor, and anti-Aging. However, the neuroprotective effects of Orientin on stroke injury have not been comprehensively evaluated. The aim of the present study was thus to investigate the neuroprotective capacity and the potential mechanisms of Cyperus esculentus L. orientin (CLO) from Cyperus esculentus L. leaves against ischemia/reperfusion (I/R) injury using standard orientin as control. For in vitro studies, we treated HT22 cells with CoCl2 as an in vitro ischemic injury model. HT22 cells in the control group were treated with CoCl2. For in vivo studies, we used rat models of middle cerebral artery occlusion, and animals that received sham surgery were used as controls. We found that CLO protected CoCl2-induced HT22 cells against ischemia/reperfusion injury by lowering lipid peroxidation and reactive oxygen species formation as well as decreasing protein oxidation. However, CLO did not reduce the release of lactate dehydrogenase nor increase the activity of superoxide dismutase. Results showed that CLO could decrease neurological deficit score, attenuate brain water content, and reduce cerebral infarct volume, leading to neuroprotection during cerebral ischemia-reperfusion injury. Our studies indicate that CLO flavonoids can be taken as a natural antioxidant and bacteriostastic substance in food and pharmaceutical industry. The molecular mechanisms of CLO could be at least partially attributed to the antioxidant properties and subsequently inhibiting activation of casepase-3. All experimental procedures and protocols were approved on May 16, 2016 by the Experimental Animal Ethics Committee of Xinjiang Medical University of China (approval No. IACUC20160516-57).

Original languageEnglish
Pages (from-to)548-556
Number of pages9
JournalNeural Regeneration Research
Volume15
Issue number3
DOIs
StatePublished - 1 Mar 2020

Fingerprint

Cyperus
Brain Ischemia
Brain Injuries
Reperfusion
Reperfusion Injury
Flavonoids
Antioxidants
Animal Care Committees
orientin
Neuroprotection
Food Industry
Middle Cerebral Artery Infarction
Wounds and Injuries
Drug Industry
Neuroprotective Agents
L-Lactate Dehydrogenase
Lipid Peroxidation
Superoxide Dismutase
Myocardial Ischemia
Reactive Oxygen Species

Keywords

  • Antioxidants
  • Caspase-3
  • Cerebral ischemia/reperfusion injury
  • Cobalt chloride
  • Cyperus esculentus L. orientin (CLO)
  • Lipid peroxidation
  • Nerve regeneration
  • Neurological deficits
  • Oxidative stress
  • Reactive oxygen species

Cite this

Jing, Si Qun ; Wang, Sai Sai ; Zhong, Rui Min ; Zhang, Jun Yan ; Wu, Jin Zi ; Tu, Yi Xian ; Pu, Yan ; Yan, Liang Jun. / Neuroprotection of Cyperus esculentus L. orientin against cerebral ischemia/reperfusion induced brain injury. In: Neural Regeneration Research. 2020 ; Vol. 15, No. 3. pp. 548-556.
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Neuroprotection of Cyperus esculentus L. orientin against cerebral ischemia/reperfusion induced brain injury. / Jing, Si Qun; Wang, Sai Sai; Zhong, Rui Min; Zhang, Jun Yan; Wu, Jin Zi; Tu, Yi Xian; Pu, Yan; Yan, Liang Jun.

In: Neural Regeneration Research, Vol. 15, No. 3, 01.03.2020, p. 548-556.

Research output: Contribution to journalArticle

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T1 - Neuroprotection of Cyperus esculentus L. orientin against cerebral ischemia/reperfusion induced brain injury

AU - Jing, Si Qun

AU - Wang, Sai Sai

AU - Zhong, Rui Min

AU - Zhang, Jun Yan

AU - Wu, Jin Zi

AU - Tu, Yi Xian

AU - Pu, Yan

AU - Yan, Liang Jun

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AB - Orientin is a flavonoid monomer. In recent years, its importance as a source of pharmacological active substance is growing rapidly due to its properties such as anti-myocardial ischemia, anti-Apoptosis, anti-radiation, anti-Tumor, and anti-Aging. However, the neuroprotective effects of Orientin on stroke injury have not been comprehensively evaluated. The aim of the present study was thus to investigate the neuroprotective capacity and the potential mechanisms of Cyperus esculentus L. orientin (CLO) from Cyperus esculentus L. leaves against ischemia/reperfusion (I/R) injury using standard orientin as control. For in vitro studies, we treated HT22 cells with CoCl2 as an in vitro ischemic injury model. HT22 cells in the control group were treated with CoCl2. For in vivo studies, we used rat models of middle cerebral artery occlusion, and animals that received sham surgery were used as controls. We found that CLO protected CoCl2-induced HT22 cells against ischemia/reperfusion injury by lowering lipid peroxidation and reactive oxygen species formation as well as decreasing protein oxidation. However, CLO did not reduce the release of lactate dehydrogenase nor increase the activity of superoxide dismutase. Results showed that CLO could decrease neurological deficit score, attenuate brain water content, and reduce cerebral infarct volume, leading to neuroprotection during cerebral ischemia-reperfusion injury. Our studies indicate that CLO flavonoids can be taken as a natural antioxidant and bacteriostastic substance in food and pharmaceutical industry. The molecular mechanisms of CLO could be at least partially attributed to the antioxidant properties and subsequently inhibiting activation of casepase-3. All experimental procedures and protocols were approved on May 16, 2016 by the Experimental Animal Ethics Committee of Xinjiang Medical University of China (approval No. IACUC20160516-57).

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KW - Lipid peroxidation

KW - Nerve regeneration

KW - Neurological deficits

KW - Oxidative stress

KW - Reactive oxygen species

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