@article{2b7caf0f44b844df8c9a73801e9d12b1,
title = "Neuroprotection of Cyperus esculentus L. orientin against cerebral ischemia/reperfusion induced brain injury",
abstract = "Orientin is a flavonoid monomer. In recent years, its importance as a source of pharmacological active substance is growing rapidly due to its properties such as anti-myocardial ischemia, anti-Apoptosis, anti-radiation, anti-Tumor, and anti-Aging. However, the neuroprotective effects of Orientin on stroke injury have not been comprehensively evaluated. The aim of the present study was thus to investigate the neuroprotective capacity and the potential mechanisms of Cyperus esculentus L. orientin (CLO) from Cyperus esculentus L. leaves against ischemia/reperfusion (I/R) injury using standard orientin as control. For in vitro studies, we treated HT22 cells with CoCl2 as an in vitro ischemic injury model. HT22 cells in the control group were treated with CoCl2. For in vivo studies, we used rat models of middle cerebral artery occlusion, and animals that received sham surgery were used as controls. We found that CLO protected CoCl2-induced HT22 cells against ischemia/reperfusion injury by lowering lipid peroxidation and reactive oxygen species formation as well as decreasing protein oxidation. However, CLO did not reduce the release of lactate dehydrogenase nor increase the activity of superoxide dismutase. Results showed that CLO could decrease neurological deficit score, attenuate brain water content, and reduce cerebral infarct volume, leading to neuroprotection during cerebral ischemia-reperfusion injury. Our studies indicate that CLO flavonoids can be taken as a natural antioxidant and bacteriostastic substance in food and pharmaceutical industry. The molecular mechanisms of CLO could be at least partially attributed to the antioxidant properties and subsequently inhibiting activation of casepase-3. All experimental procedures and protocols were approved on May 16, 2016 by the Experimental Animal Ethics Committee of Xinjiang Medical University of China (approval No. IACUC20160516-57).",
keywords = "Antioxidants, Caspase-3, Cerebral ischemia/reperfusion injury, Cobalt chloride, Cyperus esculentus L. orientin (CLO), Lipid peroxidation, Nerve regeneration, Neurological deficits, Oxidative stress, Reactive oxygen species",
author = "Jing, {Si Qun} and Wang, {Sai Sai} and Zhong, {Rui Min} and Zhang, {Jun Yan} and Wu, {Jin Zi} and Tu, {Yi Xian} and Yan Pu and Yan, {Liang Jun}",
note = "Funding Information: Funding: This work was supported by the National Natural Science Foundation of China, No. 31770385 (to SQJ). Funding Information: Acknowledgments: The authors would like to thank Dr. Wen-Jun Li (University of North Texas Health Science Center, USA) for providing the HT22 cells. Author contributions: SSW, JYZ, JZW, YXT, and YP did experiments and were involved in data collection. SQJ, RMZ, and LJY performed the experiments, interpreted data, and wrote, reviewed and edited the manuscript. Conflicts of interest: The authors declare that there are no conflicts of interest associated with this manuscript. Financial support: This work was financially supported by the National Natural Science Foundation of China, No. 31770385 (to SQJ). The funding sources had no role in study conception and design, data analysis or interpretation, paper writing or deciding to submit this paper for publication. Institutional review board statement: All experimental procedures and protocols were approved on May 16, 2016 by the Experimental Animal Ethics Committee of Xinjiang Medical University of China (approval No. IACUC20160516-57). Copyright license agreement: The Copyright License Agreement has been signed by all authors before publication. Data sharing statement: Datasets analyzed during the current study are available from the corresponding author on reasonable request. Plagiarism check: Checked twice by iThenticate. Peer review: Externally peer reviewed. Open access statement: This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. Open peer reviewer: Rong Xie, Department of Neurosurgery, Fudan University Huashan Hospital, China Additional file: Open peer review report 1. Publisher Copyright: {\textcopyright} 2020 Wolters Kluwer Medknow Publications. All rights reserved.",
year = "2020",
month = mar,
day = "1",
doi = "10.4103/1673-5374.266063",
language = "English",
volume = "15",
pages = "548--556",
journal = "Neural Regeneration Research",
issn = "1673-5374",
publisher = "Wolters Kluwer Medknow Publications",
number = "3",
}