Neuroglobin protects against nitric oxide toxicity

Kunlin Jin; Xiao Ou Mao; Lin Xie; Adil A. Khan; David A. Greenberg

doi:10.1016/j.neulet.2007.10.031

Neuroglobin protects against nitric oxide toxicity

Kunlin Jin, Xiao Ou Mao, Lin Xie, Adil A. Khan, David A. Greenberg

Institute for Healthy Aging

Research output: Contribution to journal › Article

69 Scopus citations

Abstract

Neuroglobin (Ngb) is a novel vertebrate globin expressed principally in neurons. Ngb expression is induced by hypoxia and ischemia, and Ngb protects neurons against these insults. The mechanism of Ngb's protective action is unknown, but its ability to bind NO suggests that NO scavenging might be involved. To test this hypothesis, we treated wild type and Ngb-transfected HN33 (mouse hippocampal neuron × N18TG2 neuroblastoma) cells with NO donors and compared their sensitivity to NO-induced cell death. Ngb overexpression shifted concentration-toxicity curves to the right, indicating reduced susceptibility to NO or is metabolites. The results suggest that the ability of Ngb to neutralize the neurotoxic effects of reactive nitrogen species may be an important contributor to its neuroprotective properties.

Original language	English
Pages (from-to)	135-137
Number of pages	3
Journal	Neuroscience letters
Volume	430
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2007.10.031
State	Published - 10 Jan 2008

Keywords

Hypoxia
Neuroglobin
Nitric oxide

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10.1016/j.neulet.2007.10.031

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Jin, K., Mao, X. O., Xie, L., Khan, A. A., & Greenberg, D. A. (2008). Neuroglobin protects against nitric oxide toxicity. Neuroscience letters, 430(2), 135-137. https://doi.org/10.1016/j.neulet.2007.10.031

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abstract = "Neuroglobin (Ngb) is a novel vertebrate globin expressed principally in neurons. Ngb expression is induced by hypoxia and ischemia, and Ngb protects neurons against these insults. The mechanism of Ngb's protective action is unknown, but its ability to bind NO suggests that NO scavenging might be involved. To test this hypothesis, we treated wild type and Ngb-transfected HN33 (mouse hippocampal neuron × N18TG2 neuroblastoma) cells with NO donors and compared their sensitivity to NO-induced cell death. Ngb overexpression shifted concentration-toxicity curves to the right, indicating reduced susceptibility to NO or is metabolites. The results suggest that the ability of Ngb to neutralize the neurotoxic effects of reactive nitrogen species may be an important contributor to its neuroprotective properties.",

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AB - Neuroglobin (Ngb) is a novel vertebrate globin expressed principally in neurons. Ngb expression is induced by hypoxia and ischemia, and Ngb protects neurons against these insults. The mechanism of Ngb's protective action is unknown, but its ability to bind NO suggests that NO scavenging might be involved. To test this hypothesis, we treated wild type and Ngb-transfected HN33 (mouse hippocampal neuron × N18TG2 neuroblastoma) cells with NO donors and compared their sensitivity to NO-induced cell death. Ngb overexpression shifted concentration-toxicity curves to the right, indicating reduced susceptibility to NO or is metabolites. The results suggest that the ability of Ngb to neutralize the neurotoxic effects of reactive nitrogen species may be an important contributor to its neuroprotective properties.

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