Neuroglobin Overexpression Inhibits AMPK Signaling and Promotes Cell Anabolism

Bin Cai, Wenjun Li, Xiao Ou Mao, Ali Winters, Myoung Gwi Ryou, Ran Liu, David A. Greenberg, Ning Wang, Kunlin Jin, Shaohua Yang

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Neuroglobin (Ngb) is a recently discovered globin with preferential localization to neurons. Growing evidence indicates that Ngb has distinct physiological functions separate from the oxygen storage and transport roles of other globins, such as hemoglobin and myoglobin. We found increased ATP production and decreased glycolysis in Ngb-overexpressing immortalized murine hippocampal cell line (HT-22), in parallel with inhibition of AMP-activated protein kinase (AMPK) signaling and activation of acetyl-CoA carboxylase (ACC). In addition, lipid and glycogen content was increased in Ngb-overexpressing HT-22 cells. AMPK signaling was also inhibited in the brain and heart from Ngb-overexpressing transgenic mice. Although Ngb overexpression did not change glycogen content in whole brain, glycogen synthase was activated in cortical neurons of Ngb-overexpressing mouse brain and Ngb overexpression primary neurons. Moreover, lipid and glycogen content was increased in hearts derived from Ngb-overexpressing mice. These findings suggest that Ngb functions as a metabolic regulator and enhances cellular anabolism through the inhibition of AMPK signaling.

Original languageEnglish
Pages (from-to)1254-1265
Number of pages12
JournalMolecular Neurobiology
Volume53
Issue number2
DOIs
StatePublished - 1 Mar 2016

Fingerprint

AMP-Activated Protein Kinases
Glycogen
Globins
Neurons
Brain
neuroglobin
Lipids
Acetyl-CoA Carboxylase
Glycogen Synthase
Myoglobin
Glycolysis
Transgenic Mice
Hemoglobins
Adenosine Triphosphate
Oxygen
Cell Line

Keywords

  • AMP-activated protein kinase (AMPK)
  • Acetyl-CoA carboxylase (ACC)
  • Anabolism
  • Glycogen synthase
  • Metabolism
  • Neuroglobin

Cite this

Cai, Bin ; Li, Wenjun ; Mao, Xiao Ou ; Winters, Ali ; Ryou, Myoung Gwi ; Liu, Ran ; Greenberg, David A. ; Wang, Ning ; Jin, Kunlin ; Yang, Shaohua. / Neuroglobin Overexpression Inhibits AMPK Signaling and Promotes Cell Anabolism. In: Molecular Neurobiology. 2016 ; Vol. 53, No. 2. pp. 1254-1265.
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abstract = "Neuroglobin (Ngb) is a recently discovered globin with preferential localization to neurons. Growing evidence indicates that Ngb has distinct physiological functions separate from the oxygen storage and transport roles of other globins, such as hemoglobin and myoglobin. We found increased ATP production and decreased glycolysis in Ngb-overexpressing immortalized murine hippocampal cell line (HT-22), in parallel with inhibition of AMP-activated protein kinase (AMPK) signaling and activation of acetyl-CoA carboxylase (ACC). In addition, lipid and glycogen content was increased in Ngb-overexpressing HT-22 cells. AMPK signaling was also inhibited in the brain and heart from Ngb-overexpressing transgenic mice. Although Ngb overexpression did not change glycogen content in whole brain, glycogen synthase was activated in cortical neurons of Ngb-overexpressing mouse brain and Ngb overexpression primary neurons. Moreover, lipid and glycogen content was increased in hearts derived from Ngb-overexpressing mice. These findings suggest that Ngb functions as a metabolic regulator and enhances cellular anabolism through the inhibition of AMPK signaling.",
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Cai, B, Li, W, Mao, XO, Winters, A, Ryou, MG, Liu, R, Greenberg, DA, Wang, N, Jin, K & Yang, S 2016, 'Neuroglobin Overexpression Inhibits AMPK Signaling and Promotes Cell Anabolism', Molecular Neurobiology, vol. 53, no. 2, pp. 1254-1265. https://doi.org/10.1007/s12035-014-9077-y

Neuroglobin Overexpression Inhibits AMPK Signaling and Promotes Cell Anabolism. / Cai, Bin; Li, Wenjun; Mao, Xiao Ou; Winters, Ali; Ryou, Myoung Gwi; Liu, Ran; Greenberg, David A.; Wang, Ning; Jin, Kunlin; Yang, Shaohua.

In: Molecular Neurobiology, Vol. 53, No. 2, 01.03.2016, p. 1254-1265.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Liu, Ran

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AU - Jin, Kunlin

AU - Yang, Shaohua

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