Neurogenesis of Rhesus adipose stromal cells

Soo Kyung Kang, Lorna A. Putnam, Joni Ylostalo, Ion Razvan Popescu, Jason Dufour, Andrei Belousov, Bruce A. Bunnell

Research output: Contribution to journalArticlepeer-review

152 Scopus citations


In this study, we isolated and characterized a population of non-human primate adipose tissue stromal cells (pATSCs) containing multipotent progenitor cells. We show that these pATSCs can differentiate into several mesodermal lineages, as well as neural lineage cells. For neural induction of pATSCs and non-human primate bone marrow stromal cells (pBMSCs), the cells were cultured in Neurobasal (NB) media supplemented with B27, basic fibroblast growth factor (bFGF), brain-derived neurotrophic factor (BDNF) and epidermal growth factor (EGF). After 4 days in culture, the pATSCs form compact, spheroid bodies that ultimately become neurospheres (NS). Free-floating neurospheres undergo extensive differentiation when cultured on PDL-laminin. Our data suggest that the neurogenic potential of pATSCs is markedly higher than that of pBMSCs. We have also performed microarray analysis and characterized the gene expression patterns in undifferentiated pATSCs. The direct comparison of gene expression profiles in undifferentiated pATSCs and pATSC-NS, and delineated specific members of important growth factor, signaling, cell adhesion and transcription factors families. Our data indicate that adipose tissue may be an alternative source of stem cells for therapy of central nervous system (CNS) defects.

Original languageEnglish
Pages (from-to)4289-4299
Number of pages11
JournalJournal of Cell Science
Issue number18
StatePublished - 15 Aug 2004


  • Adipose tissue
  • Microarray
  • Neural differentiation
  • Neurosphere
  • Non-human primate
  • Stem cell


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