Neural stem cell protects aged rat brain from ischemia-reperfusion injury through neurogenesis and angiogenesis

Yaohui Tang, Jixian Wang, Xiaojie Lin, Liuqing Wang, Bei Shao, Kunlin Jin, Yongting Wang, Guo Yuan Yang

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Neural stem cells (NSCs) show therapeutic potential for ischemia in young-adult animals. However, the effect of aging on NSC therapy is largely unknown. In this work, NSCs were transplanted into aged (24-month-old) and young-adult (3-month-old) rats at 1 day after stroke. Infarct volume and neurobehavioral outcomes were examined. The number of differentiated NSCs was compared in aged and young-adult ischemic rats and angiogenesis and neurogenesis were also determined. We found that aged rats developed larger infarcts than young-adult rats after ischemia (P<0.05). The neurobehavioral outcome was also worse for aged rats comparing with young-adult rats. Brain infarction and neurologic deficits were attenuated after NSC transplantation in both aged and young-adult rats. The number of survived NSCs in aged rats was similar to that of the young-adult rats (P>0.05) and most of them were differentiated into glial fibrillary acidic protein + (GFAP +) cells. More importantly, angiogenesis and neurogenesis were greatly enhanced in both aged and young-adult rats after transplantation compared with phosphate-buffered saline (PBS) control (P<0.05), accompanied by increased expression of vascular endothelial growth factor (VEGF). Our results showed that NSC therapy reduced ischemic brain injury, along with increased angiogenesis and neurogenesis in aged rats, suggesting that aging-related microenvironment does not preclude a beneficial response to NSCs transplantation during cerebral ischemia.

Original languageEnglish
Pages (from-to)1138-1147
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume34
Issue number7
DOIs
StatePublished - Jul 2014

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Neural Stem Cells
Neurogenesis
Reperfusion Injury
Brain Ischemia
Young Adult
Cell- and Tissue-Based Therapy
Ischemia
Glial Fibrillary Acidic Protein
Stem Cell Transplantation
Brain Injuries
Vascular Endothelial Growth Factor A
Transplantation
Stroke
Phosphates

Keywords

  • aged rats
  • behavioral recovery
  • ischemia
  • neural stem cell transplantation

Cite this

Tang, Yaohui ; Wang, Jixian ; Lin, Xiaojie ; Wang, Liuqing ; Shao, Bei ; Jin, Kunlin ; Wang, Yongting ; Yang, Guo Yuan. / Neural stem cell protects aged rat brain from ischemia-reperfusion injury through neurogenesis and angiogenesis. In: Journal of Cerebral Blood Flow and Metabolism. 2014 ; Vol. 34, No. 7. pp. 1138-1147.
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abstract = "Neural stem cells (NSCs) show therapeutic potential for ischemia in young-adult animals. However, the effect of aging on NSC therapy is largely unknown. In this work, NSCs were transplanted into aged (24-month-old) and young-adult (3-month-old) rats at 1 day after stroke. Infarct volume and neurobehavioral outcomes were examined. The number of differentiated NSCs was compared in aged and young-adult ischemic rats and angiogenesis and neurogenesis were also determined. We found that aged rats developed larger infarcts than young-adult rats after ischemia (P<0.05). The neurobehavioral outcome was also worse for aged rats comparing with young-adult rats. Brain infarction and neurologic deficits were attenuated after NSC transplantation in both aged and young-adult rats. The number of survived NSCs in aged rats was similar to that of the young-adult rats (P>0.05) and most of them were differentiated into glial fibrillary acidic protein + (GFAP +) cells. More importantly, angiogenesis and neurogenesis were greatly enhanced in both aged and young-adult rats after transplantation compared with phosphate-buffered saline (PBS) control (P<0.05), accompanied by increased expression of vascular endothelial growth factor (VEGF). Our results showed that NSC therapy reduced ischemic brain injury, along with increased angiogenesis and neurogenesis in aged rats, suggesting that aging-related microenvironment does not preclude a beneficial response to NSCs transplantation during cerebral ischemia.",
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Neural stem cell protects aged rat brain from ischemia-reperfusion injury through neurogenesis and angiogenesis. / Tang, Yaohui; Wang, Jixian; Lin, Xiaojie; Wang, Liuqing; Shao, Bei; Jin, Kunlin; Wang, Yongting; Yang, Guo Yuan.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 34, No. 7, 07.2014, p. 1138-1147.

Research output: Contribution to journalArticle

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AU - Tang, Yaohui

AU - Wang, Jixian

AU - Lin, Xiaojie

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