TY - JOUR
T1 - Nerve growth factor (NGF) regulation of estrogen receptors in explant cultures of the developing forebrain
AU - Miranda, Rajesh
AU - Sohrabji, Farida
AU - Singh, Meharvan
AU - Toran-Allerand, Dominique
PY - 1996/9/1
Y1 - 1996/9/1
N2 - Estrogen profoundly affects the organization of the nervous system. Its receptor, a nuclear transcription factor, is widely expressed in the developing forebrain. Earlier work established that forebrain estrogen target neurons coexpress nerve growth factor (NGF) receptors and receptor mRNA. The present study examined the regulation of forebrain estrogen receptors by NGF, using organotypic cultures of the developing cerebral cortex and basal forebrain. NGF significantly increased nuclear estrogen binding in cortical but not basal forebrain explants. Both cortical and basal forebrain explant cultures express the NGF receptor, TrkA. However, our earlier observation that developing cortical neurons, unlike basal forebrain neurons, widely coexpress mRNAs for NGF and its cognate receptors, suggests that in the present study cortical neuronal responses to exogenous NGF may have been primed by autocrine mechanisms. Alterations in nuclear estrogen binding but not estrogen receptor mRNA levels suggests that NGF may regulate cortical estrogen receptors posttranscriptionally.
AB - Estrogen profoundly affects the organization of the nervous system. Its receptor, a nuclear transcription factor, is widely expressed in the developing forebrain. Earlier work established that forebrain estrogen target neurons coexpress nerve growth factor (NGF) receptors and receptor mRNA. The present study examined the regulation of forebrain estrogen receptors by NGF, using organotypic cultures of the developing cerebral cortex and basal forebrain. NGF significantly increased nuclear estrogen binding in cortical but not basal forebrain explants. Both cortical and basal forebrain explant cultures express the NGF receptor, TrkA. However, our earlier observation that developing cortical neurons, unlike basal forebrain neurons, widely coexpress mRNAs for NGF and its cognate receptors, suggests that in the present study cortical neuronal responses to exogenous NGF may have been primed by autocrine mechanisms. Alterations in nuclear estrogen binding but not estrogen receptor mRNA levels suggests that NGF may regulate cortical estrogen receptors posttranscriptionally.
KW - cerebal cortex
KW - development
KW - estrogen receptors
KW - nerve growth factor
KW - tissue culture
UR - http://www.scopus.com/inward/record.url?scp=0029767992&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-4695(199609)31:1<77::AID-NEU7>3.0.CO;2-C
DO - 10.1002/(SICI)1097-4695(199609)31:1<77::AID-NEU7>3.0.CO;2-C
M3 - Article
C2 - 9120438
AN - SCOPUS:0029767992
SN - 0022-3034
VL - 31
SP - 77
EP - 87
JO - Journal of Neurobiology
JF - Journal of Neurobiology
IS - 1
ER -