Abstract
A simple method was described for negative gold staining of ultrathinlayer polyacrylamide gels. The protein bands were clear (unstained) and sharp while the gel matrix was pink-purple. Negative gold staining was up to five-fold more sensitive than coomassie blue, but an order of magnitude less sensitive than silver staining for protein detection. However, there were examples where negative gold staining was able to detect particular proteins not effectively stained by coomassie blue or silver.
| Original language | English |
|---|---|
| Pages (from-to) | 647-654 |
| Number of pages | 8 |
| Journal | ACTA HISTOCHEMICA ET CYTOCHEMICA |
| Volume | 19 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1 Jan 1986 |
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Negative gold staining for electrophoretic protein profile interpretations. / Budowle, Bruce; Gambel, Anne M.
In: ACTA HISTOCHEMICA ET CYTOCHEMICA, Vol. 19, No. 5, 01.01.1986, p. 647-654.Research output: Contribution to journal › Article
TY - JOUR
T1 - Negative gold staining for electrophoretic protein profile interpretations
AU - Budowle, Bruce
AU - Gambel, Anne M.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - A simple method was described for negative gold staining of ultrathinlayer polyacrylamide gels. The protein bands were clear (unstained) and sharp while the gel matrix was pink-purple. Negative gold staining was up to five-fold more sensitive than coomassie blue, but an order of magnitude less sensitive than silver staining for protein detection. However, there were examples where negative gold staining was able to detect particular proteins not effectively stained by coomassie blue or silver.
AB - A simple method was described for negative gold staining of ultrathinlayer polyacrylamide gels. The protein bands were clear (unstained) and sharp while the gel matrix was pink-purple. Negative gold staining was up to five-fold more sensitive than coomassie blue, but an order of magnitude less sensitive than silver staining for protein detection. However, there were examples where negative gold staining was able to detect particular proteins not effectively stained by coomassie blue or silver.
UR - http://www.scopus.com/inward/record.url?scp=85007763769&partnerID=8YFLogxK
U2 - 10.1267/ahc.19.647
DO - 10.1267/ahc.19.647
M3 - Article
AN - SCOPUS:85007763769
VL - 19
SP - 647
EP - 654
JO - ACTA HISTOCHEMICA ET CYTOCHEMICA
JF - ACTA HISTOCHEMICA ET CYTOCHEMICA
SN - 0044-5991
IS - 5
ER -