Negative gold staining for electrophoretic protein profile interpretations

Bruce Budowle, Anne M. Gambel

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A simple method was described for negative gold staining of ultrathinlayer polyacrylamide gels. The protein bands were clear (unstained) and sharp while the gel matrix was pink-purple. Negative gold staining was up to five-fold more sensitive than coomassie blue, but an order of magnitude less sensitive than silver staining for protein detection. However, there were examples where negative gold staining was able to detect particular proteins not effectively stained by coomassie blue or silver.

Original languageEnglish
Pages (from-to)647-654
Number of pages8
JournalACTA HISTOCHEMICA ET CYTOCHEMICA
Volume19
Issue number5
DOIs
StatePublished - 1 Jan 1986

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Negative Staining
Gold
Silver Proteins
Silver Staining
Proteins
Silver
Gels
Coomassie blue

Cite this

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Negative gold staining for electrophoretic protein profile interpretations. / Budowle, Bruce; Gambel, Anne M.

In: ACTA HISTOCHEMICA ET CYTOCHEMICA, Vol. 19, No. 5, 01.01.1986, p. 647-654.

Research output: Contribution to journalArticle

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