Negative gold staining for electrophoretic protein profile interpretations

Bruce Budowle; Anne M. Gambel

doi:10.1267/ahc.19.647

Negative gold staining for electrophoretic protein profile interpretations

Bruce Budowle, Anne M. Gambel

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Abstract

A simple method was described for negative gold staining of ultrathinlayer polyacrylamide gels. The protein bands were clear (unstained) and sharp while the gel matrix was pink-purple. Negative gold staining was up to five-fold more sensitive than coomassie blue, but an order of magnitude less sensitive than silver staining for protein detection. However, there were examples where negative gold staining was able to detect particular proteins not effectively stained by coomassie blue or silver.

Original language	English
Pages (from-to)	647-654
Number of pages	8
Journal	ACTA HISTOCHEMICA ET CYTOCHEMICA
Volume	19
Issue number	5
DOIs	https://doi.org/10.1267/ahc.19.647
State	Published - 1 Jan 1986

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AB - A simple method was described for negative gold staining of ultrathinlayer polyacrylamide gels. The protein bands were clear (unstained) and sharp while the gel matrix was pink-purple. Negative gold staining was up to five-fold more sensitive than coomassie blue, but an order of magnitude less sensitive than silver staining for protein detection. However, there were examples where negative gold staining was able to detect particular proteins not effectively stained by coomassie blue or silver.

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Negative gold staining for electrophoretic protein profile interpretations

Abstract

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