Near-infrared squaraine dyes for fluorescence enhanced surface assay

Evgenia G. Matveeva, Ewald A. Terpetschnig, Megan Stevens, Leonid Patsenker, Olga S. Kolosova, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Commercially available, near-infrared fluorescent squaraine dyes (Seta-635 and Seta-670) were covalently bound to antibodies and employed in surface enhanced immunoassay. From fluorescence intensity and lifetime changes determined for a surface which had been coated with silver nanoparticles as well as a non-coated glass surface, both labeled compounds exhibited a 15- to 20-fold enhancement of fluorescence on the silver-coated surface compared to that achieved on the non-coated surface. In addition, the fluorescence lifetime changed drastically for both labels in the case of silver-coated surfaces. The fluorescence signal enhancement obtained for the two dyes was greater than that previously recorded for Rhodamine Red-X and AlexaFluor-647 labels.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalDyes and Pigments
Volume80
Issue number1
DOIs
StatePublished - 1 Jan 2009

Keywords

  • Enhanced fluorescence immunoassays
  • Near-infrared fluorescent squaraine dyes
  • Sensors
  • Seta dyes
  • Silver island films
  • Total internal reflection

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