TY - GEN
T1 - Nanoplasmonic platforms for bioassays
AU - Gryczynski, Zygmunt
AU - Matveeva, Evgenia
AU - Shtoyko, Tanya
AU - Goldys, Ewa
AU - Schetz, John
AU - Borejdo, Julian
AU - Calander, Nils
AU - Gryczynski, Ignacy
PY - 2006/12/1
Y1 - 2006/12/1
N2 - Surface-assembled thin molecular layers and nanostructures offer unique opportunities to study processes and mechanisms of macromolecular (protein-protein or/and protein-ligand) interactions. Possibility to capture the conformational information buried in quantum-level interactions offers unique opportunities for the dynamic monitoring of biophysical and biochemical processes at the molecular level. In this report we are presenting novel developments for bioassays with enhanced sensitivity and capabilities due to quantum-photonic interactions of a fluorescent reporter with metallic nanostructures. We discus three cases of plasmons: (1) in nanometer size particles (localized plasmons and metal enhanced fluorescence); (2) in thin metallic film (surface plasmons and directional surface plasmon coupled emeission SPCE); and (3) combination of nanoparticles with continous nanosurfaces (enhanced and directional emission). We will present examples of enhanced immunoassays, background suppression, and applications of this novel technology to the simultaneous measurement of changes in conformation, orientation and mass that occur when biomolecular complexes form.
AB - Surface-assembled thin molecular layers and nanostructures offer unique opportunities to study processes and mechanisms of macromolecular (protein-protein or/and protein-ligand) interactions. Possibility to capture the conformational information buried in quantum-level interactions offers unique opportunities for the dynamic monitoring of biophysical and biochemical processes at the molecular level. In this report we are presenting novel developments for bioassays with enhanced sensitivity and capabilities due to quantum-photonic interactions of a fluorescent reporter with metallic nanostructures. We discus three cases of plasmons: (1) in nanometer size particles (localized plasmons and metal enhanced fluorescence); (2) in thin metallic film (surface plasmons and directional surface plasmon coupled emeission SPCE); and (3) combination of nanoparticles with continous nanosurfaces (enhanced and directional emission). We will present examples of enhanced immunoassays, background suppression, and applications of this novel technology to the simultaneous measurement of changes in conformation, orientation and mass that occur when biomolecular complexes form.
UR - http://www.scopus.com/inward/record.url?scp=46149087790&partnerID=8YFLogxK
U2 - 10.1109/METAMAT.2006.335048
DO - 10.1109/METAMAT.2006.335048
M3 - Conference contribution
AN - SCOPUS:46149087790
SN - 0780397746
SN - 9780780397743
T3 - Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006
BT - Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006
T2 - International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006
Y2 - 16 October 2006 through 18 October 2006
ER -