Nanoparticle effects on human platelets in vitro: A comparison between PAMAM and triazine dendrimers

Alan E. Enciso; Barry Neun; Jamie Rodriguez; Amalendu P. Ranjan; Marina A. Dobrovolskaia; Eric E. Simanek

doi:10.3390/molecules21040428

Nanoparticle effects on human platelets in vitro: A comparison between PAMAM and triazine dendrimers

Alan E. Enciso, Barry Neun, Jamie Rodriguez, Amalendu P. Ranjan, Marina A. Dobrovolskaia, Eric E. Simanek

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Abstract

Triazine and PAMAM dendrimers of similar size and number of cationic surface groups were compared for their ability to promote platelet aggregation. Triazine dendrimers (G3, G5 and G7) varied in molecular weight from 8 kDa-130 kDa and in surface groups 16-256. PAMAM dendrimers selected for comparison included G3 (7 kDa, 32 surface groups) and G6 (58 kDa, 256 surface groups). The treatment of human platelet-rich plasma (PRP) with low generation triazine dendrimers (0.01-1 μM) did not show any significant effect in human platelet aggregation in vitro; however, the treatment of PRP with larger generations promotes an effective aggregation. These results are in agreement with studies performed with PAMAM dendrimers, where large generations promote aggregation. Triazine dendrimers promote aggregation less aggressively than PAMAM dendrimers, a factor attributed to differences in cationic charge or the formation of supramolecular assemblies of dendrimers.

Original language	English
Article number	428
Journal	Molecules
Volume	21
Issue number	4
DOIs	https://doi.org/10.3390/molecules21040428
State	Published - Apr 2016

Keywords

Biocompatibility
Dendrimer
PAMAM
Platelet
Triazine

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10.3390/molecules21040428

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title = "Nanoparticle effects on human platelets in vitro: A comparison between PAMAM and triazine dendrimers",

abstract = "Triazine and PAMAM dendrimers of similar size and number of cationic surface groups were compared for their ability to promote platelet aggregation. Triazine dendrimers (G3, G5 and G7) varied in molecular weight from 8 kDa-130 kDa and in surface groups 16-256. PAMAM dendrimers selected for comparison included G3 (7 kDa, 32 surface groups) and G6 (58 kDa, 256 surface groups). The treatment of human platelet-rich plasma (PRP) with low generation triazine dendrimers (0.01-1 μM) did not show any significant effect in human platelet aggregation in vitro; however, the treatment of PRP with larger generations promotes an effective aggregation. These results are in agreement with studies performed with PAMAM dendrimers, where large generations promote aggregation. Triazine dendrimers promote aggregation less aggressively than PAMAM dendrimers, a factor attributed to differences in cationic charge or the formation of supramolecular assemblies of dendrimers.",

keywords = "Biocompatibility, Dendrimer, PAMAM, Platelet, Triazine",

author = "Enciso, {Alan E.} and Barry Neun and Jamie Rodriguez and Ranjan, {Amalendu P.} and Dobrovolskaia, {Marina A.} and Simanek, {Eric E.}",

note = "Funding Information: We thank the Robert A. Welch Foundation (P-0008) and DOD (W81XWH-12-1-0338). The study was supported in part by federal funds from the National Cancer Institute, National Institutes of Health, under contract HHSN261200800001E (M.D., B.N. and J.R.). The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. Publisher Copyright: {\textcopyright} 2016 by the authors.",

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AU - Dobrovolskaia, Marina A.

AU - Simanek, Eric E.

N1 - Funding Information: We thank the Robert A. Welch Foundation (P-0008) and DOD (W81XWH-12-1-0338). The study was supported in part by federal funds from the National Cancer Institute, National Institutes of Health, under contract HHSN261200800001E (M.D., B.N. and J.R.). The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. Publisher Copyright: © 2016 by the authors.

PY - 2016/4

Y1 - 2016/4

N2 - Triazine and PAMAM dendrimers of similar size and number of cationic surface groups were compared for their ability to promote platelet aggregation. Triazine dendrimers (G3, G5 and G7) varied in molecular weight from 8 kDa-130 kDa and in surface groups 16-256. PAMAM dendrimers selected for comparison included G3 (7 kDa, 32 surface groups) and G6 (58 kDa, 256 surface groups). The treatment of human platelet-rich plasma (PRP) with low generation triazine dendrimers (0.01-1 μM) did not show any significant effect in human platelet aggregation in vitro; however, the treatment of PRP with larger generations promotes an effective aggregation. These results are in agreement with studies performed with PAMAM dendrimers, where large generations promote aggregation. Triazine dendrimers promote aggregation less aggressively than PAMAM dendrimers, a factor attributed to differences in cationic charge or the formation of supramolecular assemblies of dendrimers.

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Nanoparticle effects on human platelets in vitro: A comparison between PAMAM and triazine dendrimers

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