N-acetylglucosamine induces white to opaque switching, a mating prerequisite in Candida albicans

Guanghua Huang, Song Yi, Nidhi Sahni, Karla J. Daniels, Srikantha Thyagarajan, David R. Soll

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135 Citations (Scopus)

Abstract

To mate, the fungal pathogen Candida albicans must undergo homozygosis at the mating-type locus and then switch from the white to opaque phenotype. Paradoxically, opaque cells were found to be unstable at physiological temperature, suggesting that mating had little chance of occurring in the host, the main niche of C. albicans. Recently, however, it was demonstrated that high levels of CO2, equivalent to those found in the host gastrointestinal tract and select tissues, induced the white to opaque switch at physiological temperature, providing a possible resolution to the paradox. Here, we demonstrate that a second signal, N-acetylglucosamine (GlcNAc), a monosaccharide produced primarily by gastrointestinal tract bacteria, also serves as a potent inducer of white to opaque switching and functions primarily through the Ras1/cAMP pathway and phosphorylated Wor1, the gene product of the master switch locus. Our results therefore suggest that signals produced by bacterial co-members of the gastrointestinal tract microbiota regulate switching and therefore mating of C. albicans.

Original languageEnglish
Article numbere1000806
JournalPLoS pathogens
Volume6
Issue number3
DOIs
StatePublished - 1 Mar 2010

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Acetylglucosamine
Candida albicans
Gastrointestinal Tract
Temperature
Monosaccharides
Bacteria
Phenotype
Genes

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Huang, Guanghua ; Yi, Song ; Sahni, Nidhi ; Daniels, Karla J. ; Thyagarajan, Srikantha ; Soll, David R. / N-acetylglucosamine induces white to opaque switching, a mating prerequisite in Candida albicans. In: PLoS pathogens. 2010 ; Vol. 6, No. 3.
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N-acetylglucosamine induces white to opaque switching, a mating prerequisite in Candida albicans. / Huang, Guanghua; Yi, Song; Sahni, Nidhi; Daniels, Karla J.; Thyagarajan, Srikantha; Soll, David R.

In: PLoS pathogens, Vol. 6, No. 3, e1000806, 01.03.2010.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Soll, David R.

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