Myoglobin immunoassay utilizing directional surface plasmon-coupled emission

Evgenia Matveeva, Zygmunt Gryczynski, Ignacy Gryczynski, Joanna Malicka, Joseph R. Lakowicz

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

We described an immunoassay for the cardiac marker myoglobin on a thin silver mirror surface using surface plasmon-coupled emission (SPCE). SPCE occurs for fluorophores in proximity (within ∼200 nm) of a thin metal film (in our case, silver) and results in a highly directional radiation through a glass substrate at a well-defined angle from the normal axis. We used the effect of SPCE to develop a myoglobin immunoassay on the silver mirror surface deposited on a glass substrate. Binding of the labeled anti-myoglobin antibodies led to the enhanced fluorescence emission at a specific angle of 72°. The directional and enhanced directional fluorescence emission enables detection of myoglobin over a wide range of concentrations from subnormal to the elevated level of this cardiac marker. Utilizing SPCE allowed us also to demonstrate significant background suppression (from serum or whole blood) in the myoglobin immunoassay. We expect SPCE to become a powerful technique for performing immunoassays for many biomarkers in surface-bound assays.

Original languageEnglish
Pages (from-to)6287-6292
Number of pages6
JournalAnalytical Chemistry
Volume76
Issue number21
DOIs
StatePublished - 1 Nov 2004

Fingerprint

Myoglobin
Silver
Mirrors
Fluorescence
Glass
Fluorophores
Biomarkers
Substrates
Assays
Blood
Metals
Radiation
Antibodies

Cite this

Matveeva, Evgenia ; Gryczynski, Zygmunt ; Gryczynski, Ignacy ; Malicka, Joanna ; Lakowicz, Joseph R. / Myoglobin immunoassay utilizing directional surface plasmon-coupled emission. In: Analytical Chemistry. 2004 ; Vol. 76, No. 21. pp. 6287-6292.
@article{e2eed37e84dd431d95b5d2b4efe9d3ba,
title = "Myoglobin immunoassay utilizing directional surface plasmon-coupled emission",
abstract = "We described an immunoassay for the cardiac marker myoglobin on a thin silver mirror surface using surface plasmon-coupled emission (SPCE). SPCE occurs for fluorophores in proximity (within ∼200 nm) of a thin metal film (in our case, silver) and results in a highly directional radiation through a glass substrate at a well-defined angle from the normal axis. We used the effect of SPCE to develop a myoglobin immunoassay on the silver mirror surface deposited on a glass substrate. Binding of the labeled anti-myoglobin antibodies led to the enhanced fluorescence emission at a specific angle of 72°. The directional and enhanced directional fluorescence emission enables detection of myoglobin over a wide range of concentrations from subnormal to the elevated level of this cardiac marker. Utilizing SPCE allowed us also to demonstrate significant background suppression (from serum or whole blood) in the myoglobin immunoassay. We expect SPCE to become a powerful technique for performing immunoassays for many biomarkers in surface-bound assays.",
author = "Evgenia Matveeva and Zygmunt Gryczynski and Ignacy Gryczynski and Joanna Malicka and Lakowicz, {Joseph R.}",
year = "2004",
month = "11",
day = "1",
doi = "10.1021/ac0491612",
language = "English",
volume = "76",
pages = "6287--6292",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "21",

}

Myoglobin immunoassay utilizing directional surface plasmon-coupled emission. / Matveeva, Evgenia; Gryczynski, Zygmunt; Gryczynski, Ignacy; Malicka, Joanna; Lakowicz, Joseph R.

In: Analytical Chemistry, Vol. 76, No. 21, 01.11.2004, p. 6287-6292.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Myoglobin immunoassay utilizing directional surface plasmon-coupled emission

AU - Matveeva, Evgenia

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

AU - Malicka, Joanna

AU - Lakowicz, Joseph R.

PY - 2004/11/1

Y1 - 2004/11/1

N2 - We described an immunoassay for the cardiac marker myoglobin on a thin silver mirror surface using surface plasmon-coupled emission (SPCE). SPCE occurs for fluorophores in proximity (within ∼200 nm) of a thin metal film (in our case, silver) and results in a highly directional radiation through a glass substrate at a well-defined angle from the normal axis. We used the effect of SPCE to develop a myoglobin immunoassay on the silver mirror surface deposited on a glass substrate. Binding of the labeled anti-myoglobin antibodies led to the enhanced fluorescence emission at a specific angle of 72°. The directional and enhanced directional fluorescence emission enables detection of myoglobin over a wide range of concentrations from subnormal to the elevated level of this cardiac marker. Utilizing SPCE allowed us also to demonstrate significant background suppression (from serum or whole blood) in the myoglobin immunoassay. We expect SPCE to become a powerful technique for performing immunoassays for many biomarkers in surface-bound assays.

AB - We described an immunoassay for the cardiac marker myoglobin on a thin silver mirror surface using surface plasmon-coupled emission (SPCE). SPCE occurs for fluorophores in proximity (within ∼200 nm) of a thin metal film (in our case, silver) and results in a highly directional radiation through a glass substrate at a well-defined angle from the normal axis. We used the effect of SPCE to develop a myoglobin immunoassay on the silver mirror surface deposited on a glass substrate. Binding of the labeled anti-myoglobin antibodies led to the enhanced fluorescence emission at a specific angle of 72°. The directional and enhanced directional fluorescence emission enables detection of myoglobin over a wide range of concentrations from subnormal to the elevated level of this cardiac marker. Utilizing SPCE allowed us also to demonstrate significant background suppression (from serum or whole blood) in the myoglobin immunoassay. We expect SPCE to become a powerful technique for performing immunoassays for many biomarkers in surface-bound assays.

UR - http://www.scopus.com/inward/record.url?scp=7644240095&partnerID=8YFLogxK

U2 - 10.1021/ac0491612

DO - 10.1021/ac0491612

M3 - Article

C2 - 15516120

AN - SCOPUS:7644240095

VL - 76

SP - 6287

EP - 6292

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 21

ER -