Myoglobin immunoassay based on metal particle-enhanced fluorescence

Evgenia G. Matveeva, Zygmunt Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

Enhanced fluorescence on silver island films (SIFs) is utilized to develop a sandwich-format immunoassay for the cardiac marker myoglobin (Myo). Myoglobin was first captured on surfaces coated with anti-Myo antibodies; the surface was then incubated with fluorescently labeled anti-Myo antibodies. The system was examined on glass surfaces and on SIFs. We observed the enhancement of the signal from SIFs in the range of 10-15-fold if compared to the signal from the glass substrate not modified with a SIF. A kinetic immunoassay for Myo on SIF-modified surface results in a decreased background signal. The initial results show that it is possible to detect Myoglobin concentrations below 50 ng/mL, which is lower than clinical cut-off for Myoglobin in healthy patients. We suggest the use of SIF-modified substrates for increasing the sensitivity of surface assays with fluorescence detection.

Original languageEnglish
Pages (from-to)26-35
Number of pages10
JournalJournal of Immunological Methods
Volume302
Issue number1-2
DOIs
StatePublished - Jul 2005

Keywords

  • Cardiac markers
  • Fluorescence immunoassay
  • Metal-enhanced fluorescence
  • Myoglobin
  • Nano-size metallic particles
  • Silver island films

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