Myoglobin immunoassay based on metal particle-enhanced fluorescence

Evgenia G. Matveeva, Zygmunt Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Enhanced fluorescence on silver island films (SIFs) is utilized to develop a sandwich-format immunoassay for the cardiac marker myoglobin (Myo). Myoglobin was first captured on surfaces coated with anti-Myo antibodies; the surface was then incubated with fluorescently labeled anti-Myo antibodies. The system was examined on glass surfaces and on SIFs. We observed the enhancement of the signal from SIFs in the range of 10-15-fold if compared to the signal from the glass substrate not modified with a SIF. A kinetic immunoassay for Myo on SIF-modified surface results in a decreased background signal. The initial results show that it is possible to detect Myoglobin concentrations below 50 ng/mL, which is lower than clinical cut-off for Myoglobin in healthy patients. We suggest the use of SIF-modified substrates for increasing the sensitivity of surface assays with fluorescence detection.

Original languageEnglish
Pages (from-to)26-35
Number of pages10
JournalJournal of Immunological Methods
Volume302
Issue number1-2
DOIs
StatePublished - 1 Jul 2005

Fingerprint

Myoglobin
Immunoassay
Silver
Islands
Fluorescence
Metals
Glass
Anti-Idiotypic Antibodies

Keywords

  • Cardiac markers
  • Fluorescence immunoassay
  • Metal-enhanced fluorescence
  • Myoglobin
  • Nano-size metallic particles
  • Silver island films

Cite this

Matveeva, Evgenia G. ; Gryczynski, Zygmunt ; Lakowicz, Joseph R. / Myoglobin immunoassay based on metal particle-enhanced fluorescence. In: Journal of Immunological Methods. 2005 ; Vol. 302, No. 1-2. pp. 26-35.
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Myoglobin immunoassay based on metal particle-enhanced fluorescence. / Matveeva, Evgenia G.; Gryczynski, Zygmunt; Lakowicz, Joseph R.

In: Journal of Immunological Methods, Vol. 302, No. 1-2, 01.07.2005, p. 26-35.

Research output: Contribution to journalArticle

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AU - Matveeva, Evgenia G.

AU - Gryczynski, Zygmunt

AU - Lakowicz, Joseph R.

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AB - Enhanced fluorescence on silver island films (SIFs) is utilized to develop a sandwich-format immunoassay for the cardiac marker myoglobin (Myo). Myoglobin was first captured on surfaces coated with anti-Myo antibodies; the surface was then incubated with fluorescently labeled anti-Myo antibodies. The system was examined on glass surfaces and on SIFs. We observed the enhancement of the signal from SIFs in the range of 10-15-fold if compared to the signal from the glass substrate not modified with a SIF. A kinetic immunoassay for Myo on SIF-modified surface results in a decreased background signal. The initial results show that it is possible to detect Myoglobin concentrations below 50 ng/mL, which is lower than clinical cut-off for Myoglobin in healthy patients. We suggest the use of SIF-modified substrates for increasing the sensitivity of surface assays with fluorescence detection.

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KW - Fluorescence immunoassay

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