Abstract
Enhanced fluorescence on silver island films (SIFs) is utilized to develop a sandwich-format immunoassay for the cardiac marker myoglobin (Myo). Myoglobin was first captured on surfaces coated with anti-Myo antibodies; the surface was then incubated with fluorescently labeled anti-Myo antibodies. The system was examined on glass surfaces and on SIFs. We observed the enhancement of the signal from SIFs in the range of 10-15-fold if compared to the signal from the glass substrate not modified with a SIF. A kinetic immunoassay for Myo on SIF-modified surface results in a decreased background signal. The initial results show that it is possible to detect Myoglobin concentrations below 50 ng/mL, which is lower than clinical cut-off for Myoglobin in healthy patients. We suggest the use of SIF-modified substrates for increasing the sensitivity of surface assays with fluorescence detection.
Original language | English |
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Pages (from-to) | 26-35 |
Number of pages | 10 |
Journal | Journal of Immunological Methods |
Volume | 302 |
Issue number | 1-2 |
DOIs | |
State | Published - Jul 2005 |
Keywords
- Cardiac markers
- Fluorescence immunoassay
- Metal-enhanced fluorescence
- Myoglobin
- Nano-size metallic particles
- Silver island films