Multiplex amplification and typing procedure for the loci D1S80 and amelogenin

Bruce Budowle, Barbara W. Koons, Jacqueline D. Errera

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

A method has been developed that enables multiplex amplification and simultaneous typing of the loci D1S80 and amelogenin using discontinuous polyacrylamide gel electrophoresis and silver staining. The protocol is sensitive, simple, rapid, and relatively inexpensive. The results of the multiplex analysis of the D1S80 and amelogenin loci were comparable to those obtained when each locus was analyzed individually. A small validation study was undertaken to evaluate the forensic applicability of this multiplex system. The data demonstrate that DNA exposed to a variety of environmental insults yields reliable multiplex typing results.

Original languageEnglish
Pages (from-to)660-663
Number of pages4
JournalJournal of Forensic Sciences
Volume41
Issue number4
StatePublished - 1 Jul 1996

Keywords

  • D1S80
  • DNA typing
  • amelogenin
  • forensic science
  • multiplex amplification
  • polymerase chain reaction
  • sex determination
  • validation studies

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