TY - JOUR
T1 - Multiphoton excitation of fluorescence near metallic particles
T2 - Enhanced and localized excitation
AU - Gryczynski, Ignacy
AU - Malicka, Joanna
AU - Shen, Yibing
AU - Gryczynski, Zygmunt
AU - Lakowicz, Joseph R.
PY - 2002/3/7
Y1 - 2002/3/7
N2 - We examined the effects of silver island films on multiphoton excitation of rhodamine derivatives and biochemical fluorophores. The fluorophores were studied in approximately 1 μm thick samples between two quartz slides coated with silver islands. Intensity and lifetime measurements of rhodamine B demonstrated enhanced two-photon excitation near the metal particles. Time-resolved measurements showed a decreased lifetime of RhB with two-photon excitation as compared to unsilvered quartz slides, which indicates the two-photon excitation was localized near the metal particles. Additionally, rhodamine B showed increased photostability with two-photon excitation near metal particles, which is consistent with the shorter lifetime. For lower quantum yield fluorophores such as rose bengal, eosin, 1-anilinonaphthalene-8-sulfonic acid, and coumarin 152, we found the emission from the micron thick sample was almost completely due to the fluorophores near the silver particles, perhaps to within 300 Å of the metal surfaces. These results suggest the use of metal particles for localized multiphoton excitation of biological macromolecules or cells. Additionally, one can imagine metal colloids with bound fluorophores being selectively excited when placed in highly fluorescent samples such as tissue samples.
AB - We examined the effects of silver island films on multiphoton excitation of rhodamine derivatives and biochemical fluorophores. The fluorophores were studied in approximately 1 μm thick samples between two quartz slides coated with silver islands. Intensity and lifetime measurements of rhodamine B demonstrated enhanced two-photon excitation near the metal particles. Time-resolved measurements showed a decreased lifetime of RhB with two-photon excitation as compared to unsilvered quartz slides, which indicates the two-photon excitation was localized near the metal particles. Additionally, rhodamine B showed increased photostability with two-photon excitation near metal particles, which is consistent with the shorter lifetime. For lower quantum yield fluorophores such as rose bengal, eosin, 1-anilinonaphthalene-8-sulfonic acid, and coumarin 152, we found the emission from the micron thick sample was almost completely due to the fluorophores near the silver particles, perhaps to within 300 Å of the metal surfaces. These results suggest the use of metal particles for localized multiphoton excitation of biological macromolecules or cells. Additionally, one can imagine metal colloids with bound fluorophores being selectively excited when placed in highly fluorescent samples such as tissue samples.
UR - http://www.scopus.com/inward/record.url?scp=0037035134&partnerID=8YFLogxK
U2 - 10.1021/jp013013n
DO - 10.1021/jp013013n
M3 - Article
AN - SCOPUS:0037035134
SN - 1520-6106
VL - 106
SP - 2191
EP - 2195
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
IS - 9
ER -