Multigenerational amplification of a reference ladder for alleles at locus D1S80

F. S. Baechtel, J. B. Smerick, K. W. Presley, B. Budowle

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

The interlaboratory typing of DNA specimens that have been amplified at the D1S80 locus necessitates the use of a standard allelic reference ladder. This communication describes a technique in which individual, amplified alleles are isolated, combined, and amplified by PCR to produce a functional reference ladder composed of many of the alleles that occur at this locus. The amplified ladder can serve directly as a template source for production of the next generation of reference ladder. This process, in which each amplified ladder serves as the template for the next has been carried through multiple generations.

Original languageEnglish
Pages (from-to)1176-1182
Number of pages7
JournalJournal of Forensic Sciences
Volume38
Issue number5
DOIs
StatePublished - 1993

Keywords

  • Allelic ladder
  • Polyacrylamide gel electrophoresis
  • locus D1S80
  • pathology and biology

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