Multicolor fluoroimmunoassays based on surface plasmon-coupled emission

Evgenia G. Matveeva; Joanna Malicka; Ignacy Gryczynski; Joseph R. Lakowicz; Zygmunt Gryczynski

doi:10.1117/12.593430

Multicolor fluoroimmunoassays based on surface plasmon-coupled emission

Evgenia G. Matveeva, Joanna Malicka, Ignacy Gryczynski, Joseph R. Lakowicz, Zygmunt Gryczynski

Research output: Contribution to journal › Conference article › peer-review

Abstract

We present a new method for multi-color fluoroimmunoassays based on directional surface plasmon-coupled emission (SPCE). SPCE is coupling of excited fluorophores with a nearby thin metal film (silver) resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagates through the prism depends on emission wavelength and makes possible measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using two antibodies labeled with different fluorophores, binding to an antigen protein immobilized on the silver surface. We observed independent emission at a different angle on the glass prism, resulting of the surface binding of each antibody. This methodology can be readily extended to 3 or more fluorophores. This technology presents opportunity to develop highly sensitive multiplex assay format for biological agents' detection.

Original language	English
Article number	56
Pages (from-to)	316-325
Number of pages	10
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5692
DOIs	https://doi.org/10.1117/12.593430
State	Published - 21 Jul 2005
Event	Advanced Biomedical and Clinical Diagnostic Systems III - San Jose, CA, United States Duration: 23 Jan 2005 → 26 Jan 2005

Keywords

Directional fluorescence
Fluorescence immunoassay
Multi-color assay
Surface plasmon-coupled emission

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10.1117/12.593430

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title = "Multicolor fluoroimmunoassays based on surface plasmon-coupled emission",

abstract = "We present a new method for multi-color fluoroimmunoassays based on directional surface plasmon-coupled emission (SPCE). SPCE is coupling of excited fluorophores with a nearby thin metal film (silver) resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagates through the prism depends on emission wavelength and makes possible measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using two antibodies labeled with different fluorophores, binding to an antigen protein immobilized on the silver surface. We observed independent emission at a different angle on the glass prism, resulting of the surface binding of each antibody. This methodology can be readily extended to 3 or more fluorophores. This technology presents opportunity to develop highly sensitive multiplex assay format for biological agents' detection.",

keywords = "Directional fluorescence, Fluorescence immunoassay, Multi-color assay, Surface plasmon-coupled emission",

author = "Matveeva, {Evgenia G.} and Joanna Malicka and Ignacy Gryczynski and Lakowicz, {Joseph R.} and Zygmunt Gryczynski",

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TY - JOUR

T1 - Multicolor fluoroimmunoassays based on surface plasmon-coupled emission

AU - Matveeva, Evgenia G.

AU - Malicka, Joanna

AU - Gryczynski, Ignacy

AU - Lakowicz, Joseph R.

AU - Gryczynski, Zygmunt

PY - 2005/7/21

Y1 - 2005/7/21

N2 - We present a new method for multi-color fluoroimmunoassays based on directional surface plasmon-coupled emission (SPCE). SPCE is coupling of excited fluorophores with a nearby thin metal film (silver) resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagates through the prism depends on emission wavelength and makes possible measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using two antibodies labeled with different fluorophores, binding to an antigen protein immobilized on the silver surface. We observed independent emission at a different angle on the glass prism, resulting of the surface binding of each antibody. This methodology can be readily extended to 3 or more fluorophores. This technology presents opportunity to develop highly sensitive multiplex assay format for biological agents' detection.

AB - We present a new method for multi-color fluoroimmunoassays based on directional surface plasmon-coupled emission (SPCE). SPCE is coupling of excited fluorophores with a nearby thin metal film (silver) resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagates through the prism depends on emission wavelength and makes possible measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using two antibodies labeled with different fluorophores, binding to an antigen protein immobilized on the silver surface. We observed independent emission at a different angle on the glass prism, resulting of the surface binding of each antibody. This methodology can be readily extended to 3 or more fluorophores. This technology presents opportunity to develop highly sensitive multiplex assay format for biological agents' detection.

KW - Directional fluorescence

KW - Fluorescence immunoassay

KW - Multi-color assay

KW - Surface plasmon-coupled emission

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DO - 10.1117/12.593430

M3 - Conference article

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VL - 5692

SP - 316

EP - 325

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

M1 - 56

Y2 - 23 January 2005 through 26 January 2005

ER -

Multicolor fluoroimmunoassays based on surface plasmon-coupled emission

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