Multicolor fluoroimmunoassays based on surface plasmon-coupled emission

Evgenia G. Matveeva, Joanna Malicka, Ignacy Gryczynski, Joseph R. Lakowicz, Zygmunt Gryczynski

Research output: Contribution to journalConference article

Abstract

We present a new method for multi-color fluoroimmunoassays based on directional surface plasmon-coupled emission (SPCE). SPCE is coupling of excited fluorophores with a nearby thin metal film (silver) resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagates through the prism depends on emission wavelength and makes possible measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using two antibodies labeled with different fluorophores, binding to an antigen protein immobilized on the silver surface. We observed independent emission at a different angle on the glass prism, resulting of the surface binding of each antibody. This methodology can be readily extended to 3 or more fluorophores. This technology presents opportunity to develop highly sensitive multiplex assay format for biological agents' detection.

Original languageEnglish
Article number56
Pages (from-to)316-325
Number of pages10
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5692
DOIs
StatePublished - 21 Jul 2005
EventAdvanced Biomedical and Clinical Diagnostic Systems III - San Jose, CA, United States
Duration: 23 Jan 200526 Jan 2005

Fingerprint

Fluoroimmunoassay
Silver
Glass
Fluorophores
Immobilized Proteins
Antibodies
Biological Factors
Prisms
Color
Metals
Radiation
Technology
antibodies
Antigens
Wavelength
prisms
silver
Assays
glass
antigens

Keywords

  • Directional fluorescence
  • Fluorescence immunoassay
  • Multi-color assay
  • Surface plasmon-coupled emission

Cite this

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title = "Multicolor fluoroimmunoassays based on surface plasmon-coupled emission",
abstract = "We present a new method for multi-color fluoroimmunoassays based on directional surface plasmon-coupled emission (SPCE). SPCE is coupling of excited fluorophores with a nearby thin metal film (silver) resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagates through the prism depends on emission wavelength and makes possible measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using two antibodies labeled with different fluorophores, binding to an antigen protein immobilized on the silver surface. We observed independent emission at a different angle on the glass prism, resulting of the surface binding of each antibody. This methodology can be readily extended to 3 or more fluorophores. This technology presents opportunity to develop highly sensitive multiplex assay format for biological agents' detection.",
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Multicolor fluoroimmunoassays based on surface plasmon-coupled emission. / Matveeva, Evgenia G.; Malicka, Joanna; Gryczynski, Ignacy; Lakowicz, Joseph R.; Gryczynski, Zygmunt.

In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 5692, 56, 21.07.2005, p. 316-325.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Multicolor fluoroimmunoassays based on surface plasmon-coupled emission

AU - Matveeva, Evgenia G.

AU - Malicka, Joanna

AU - Gryczynski, Ignacy

AU - Lakowicz, Joseph R.

AU - Gryczynski, Zygmunt

PY - 2005/7/21

Y1 - 2005/7/21

N2 - We present a new method for multi-color fluoroimmunoassays based on directional surface plasmon-coupled emission (SPCE). SPCE is coupling of excited fluorophores with a nearby thin metal film (silver) resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagates through the prism depends on emission wavelength and makes possible measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using two antibodies labeled with different fluorophores, binding to an antigen protein immobilized on the silver surface. We observed independent emission at a different angle on the glass prism, resulting of the surface binding of each antibody. This methodology can be readily extended to 3 or more fluorophores. This technology presents opportunity to develop highly sensitive multiplex assay format for biological agents' detection.

AB - We present a new method for multi-color fluoroimmunoassays based on directional surface plasmon-coupled emission (SPCE). SPCE is coupling of excited fluorophores with a nearby thin metal film (silver) resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagates through the prism depends on emission wavelength and makes possible measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using two antibodies labeled with different fluorophores, binding to an antigen protein immobilized on the silver surface. We observed independent emission at a different angle on the glass prism, resulting of the surface binding of each antibody. This methodology can be readily extended to 3 or more fluorophores. This technology presents opportunity to develop highly sensitive multiplex assay format for biological agents' detection.

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KW - Fluorescence immunoassay

KW - Multi-color assay

KW - Surface plasmon-coupled emission

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JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

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