Multi-pulse pumping for far-field super-resolution imaging

Sebastian Requena, Sangram Raut, Hung Doan, Joe Kimball, Rafal Fudala, Julian Borejdo, Ignacy Gryczynski, Yuri Strzhemechny, Zygmunt Gryczynski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recently, far-field optical imaging with a resolution significantly beyond diffraction limit has attracted tremendous attention allowing for high resolution imaging in living objects. Various methods have been proposed that are divided in to two basic approaches; deterministic super-resolution like STED or RESOLFT and stochastic super-resolution like PALM or STORM. We propose to achieve super-resolution in far-field fluorescence imaging by the use of controllable (on-demand) bursts of pulses that can change the fluorescence signal of long-lived component over one order of magnitude. We demonstrate that two beads, one labeled with a long-lived dye and another with a short-lived dye, separated by a distance lower than 100 nm can be easily resolved in a single experiment. The proposed method can be used to separate two biological structures in a cell by targeting them with two antibodies labeled with long-lived and short-lived fluorophores.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging IX
EditorsJorg Enderlein, Ingo Gregor, Zygmunt Karol Gryczynski, Rainer Erdmann, Felix Koberling
PublisherSPIE
ISBN (Electronic)9781628419481
DOIs
StatePublished - 1 Jan 2016
EventSingle Molecule Spectroscopy and Superresolution Imaging IX - San Francisco, United States
Duration: 13 Feb 201614 Feb 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9714
ISSN (Print)1605-7422

Other

OtherSingle Molecule Spectroscopy and Superresolution Imaging IX
CountryUnited States
CitySan Francisco
Period13/02/1614/02/16

Keywords

  • Super-resolution
  • microscopy
  • multi-pulse pumping

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  • Cite this

    Requena, S., Raut, S., Doan, H., Kimball, J., Fudala, R., Borejdo, J., Gryczynski, I., Strzhemechny, Y., & Gryczynski, Z. (2016). Multi-pulse pumping for far-field super-resolution imaging. In J. Enderlein, I. Gregor, Z. K. Gryczynski, R. Erdmann, & F. Koberling (Eds.), Single Molecule Spectroscopy and Superresolution Imaging IX [97140F] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9714). SPIE. https://doi.org/10.1117/12.2221921