Multi-pulse pumping for far-field super-resolution imaging

Sebastian Requena, Sangram Raut, Hung Doan, Joe Kimball, Rafal Fudala, Julian Borejdo, Ignacy Gryczynski, Yuri Strzhemechny, Zygmunt Gryczynski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recently, far-field optical imaging with a resolution significantly beyond diffraction limit has attracted tremendous attention allowing for high resolution imaging in living objects. Various methods have been proposed that are divided in to two basic approaches; deterministic super-resolution like STED or RESOLFT and stochastic super-resolution like PALM or STORM. We propose to achieve super-resolution in far-field fluorescence imaging by the use of controllable (on-demand) bursts of pulses that can change the fluorescence signal of long-lived component over one order of magnitude. We demonstrate that two beads, one labeled with a long-lived dye and another with a short-lived dye, separated by a distance lower than 100 nm can be easily resolved in a single experiment. The proposed method can be used to separate two biological structures in a cell by targeting them with two antibodies labeled with long-lived and short-lived fluorophores.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging IX
EditorsJorg Enderlein, Ingo Gregor, Zygmunt Karol Gryczynski, Rainer Erdmann, Felix Koberling
PublisherSPIE
ISBN (Electronic)9781628419481
DOIs
StatePublished - 1 Jan 2016
EventSingle Molecule Spectroscopy and Superresolution Imaging IX - San Francisco, United States
Duration: 13 Feb 201614 Feb 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9714
ISSN (Print)1605-7422

Other

OtherSingle Molecule Spectroscopy and Superresolution Imaging IX
CountryUnited States
CitySan Francisco
Period13/02/1614/02/16

Fingerprint

Optical Imaging
far fields
pumping
Coloring Agents
Imaging techniques
Dyes
pulses
Fluorescence
Fluorophores
dyes
Antibodies
fluorescence
Diffraction
antibodies
beads
bursts
high resolution
cells
Experiments
diffraction

Keywords

  • Super-resolution
  • microscopy
  • multi-pulse pumping

Cite this

Requena, S., Raut, S., Doan, H., Kimball, J., Fudala, R., Borejdo, J., ... Gryczynski, Z. (2016). Multi-pulse pumping for far-field super-resolution imaging. In J. Enderlein, I. Gregor, Z. K. Gryczynski, R. Erdmann, & F. Koberling (Eds.), Single Molecule Spectroscopy and Superresolution Imaging IX [97140F] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9714). SPIE. https://doi.org/10.1117/12.2221921
Requena, Sebastian ; Raut, Sangram ; Doan, Hung ; Kimball, Joe ; Fudala, Rafal ; Borejdo, Julian ; Gryczynski, Ignacy ; Strzhemechny, Yuri ; Gryczynski, Zygmunt. / Multi-pulse pumping for far-field super-resolution imaging. Single Molecule Spectroscopy and Superresolution Imaging IX. editor / Jorg Enderlein ; Ingo Gregor ; Zygmunt Karol Gryczynski ; Rainer Erdmann ; Felix Koberling. SPIE, 2016. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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Requena, S, Raut, S, Doan, H, Kimball, J, Fudala, R, Borejdo, J, Gryczynski, I, Strzhemechny, Y & Gryczynski, Z 2016, Multi-pulse pumping for far-field super-resolution imaging. in J Enderlein, I Gregor, ZK Gryczynski, R Erdmann & F Koberling (eds), Single Molecule Spectroscopy and Superresolution Imaging IX., 97140F, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9714, SPIE, Single Molecule Spectroscopy and Superresolution Imaging IX, San Francisco, United States, 13/02/16. https://doi.org/10.1117/12.2221921

Multi-pulse pumping for far-field super-resolution imaging. / Requena, Sebastian; Raut, Sangram; Doan, Hung; Kimball, Joe; Fudala, Rafal; Borejdo, Julian; Gryczynski, Ignacy; Strzhemechny, Yuri; Gryczynski, Zygmunt.

Single Molecule Spectroscopy and Superresolution Imaging IX. ed. / Jorg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling. SPIE, 2016. 97140F (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9714).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Raut, Sangram

AU - Doan, Hung

AU - Kimball, Joe

AU - Fudala, Rafal

AU - Borejdo, Julian

AU - Gryczynski, Ignacy

AU - Strzhemechny, Yuri

AU - Gryczynski, Zygmunt

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AB - Recently, far-field optical imaging with a resolution significantly beyond diffraction limit has attracted tremendous attention allowing for high resolution imaging in living objects. Various methods have been proposed that are divided in to two basic approaches; deterministic super-resolution like STED or RESOLFT and stochastic super-resolution like PALM or STORM. We propose to achieve super-resolution in far-field fluorescence imaging by the use of controllable (on-demand) bursts of pulses that can change the fluorescence signal of long-lived component over one order of magnitude. We demonstrate that two beads, one labeled with a long-lived dye and another with a short-lived dye, separated by a distance lower than 100 nm can be easily resolved in a single experiment. The proposed method can be used to separate two biological structures in a cell by targeting them with two antibodies labeled with long-lived and short-lived fluorophores.

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A2 - Koberling, Felix

PB - SPIE

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Requena S, Raut S, Doan H, Kimball J, Fudala R, Borejdo J et al. Multi-pulse pumping for far-field super-resolution imaging. In Enderlein J, Gregor I, Gryczynski ZK, Erdmann R, Koberling F, editors, Single Molecule Spectroscopy and Superresolution Imaging IX. SPIE. 2016. 97140F. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2221921