Multi-pulse pumping for far-field super-resolution imaging

Sebastian Requena, Sangram Limbaji Raut, Hung Doan, Joe Kimball, Rafal Fudala, Julian Borejdo, Ignacy Gryczynski, Yuri Strzhemechny, Zygmunt Gryczynski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recently, far-field optical imaging with a resolution significantly beyond diffraction limit has attracted tremendous attention allowing for high resolution imaging in living objects. Various methods have been proposed that are divided in to two basic approaches; deterministic super-resolution like STED or RESOLFT and stochastic super-resolution like PALM or STORM. We propose to achieve super-resolution in far-field fluorescence imaging by the use of controllable (on-demand) bursts of pulses that can change the fluorescence signal of long-lived component over one order of magnitude. We demonstrate that two beads, one labeled with a long-lived dye and another with a short-lived dye, separated by a distance lower than 100 nm can be easily resolved in a single experiment. The proposed method can be used to separate two biological structures in a cell by targeting them with two antibodies labeled with long-lived and short-lived fluorophores.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging IX
PublisherSPIE
Volume9714
ISBN (Electronic)9781628419481
DOIs
StatePublished - 1 Jan 2016
EventSingle Molecule Spectroscopy and Superresolution Imaging IX - San Francisco, United States
Duration: 13 Feb 201614 Feb 2016

Other

OtherSingle Molecule Spectroscopy and Superresolution Imaging IX
CountryUnited States
CitySan Francisco
Period13/02/1614/02/16

Fingerprint

Optical Imaging
far fields
pumping
Coloring Agents
Imaging techniques
Dyes
pulses
Fluorescence
Fluorophores
dyes
Antibodies
fluorescence
Diffraction
antibodies
beads
bursts
high resolution
cells
Experiments
diffraction

Keywords

  • microscopy
  • multi-pulse pumping
  • Super-resolution

Cite this

Requena, S., Raut, S. L., Doan, H., Kimball, J., Fudala, R., Borejdo, J., ... Gryczynski, Z. (2016). Multi-pulse pumping for far-field super-resolution imaging. In Single Molecule Spectroscopy and Superresolution Imaging IX (Vol. 9714). [97140F] SPIE. https://doi.org/10.1117/12.2221921
Requena, Sebastian ; Raut, Sangram Limbaji ; Doan, Hung ; Kimball, Joe ; Fudala, Rafal ; Borejdo, Julian ; Gryczynski, Ignacy ; Strzhemechny, Yuri ; Gryczynski, Zygmunt. / Multi-pulse pumping for far-field super-resolution imaging. Single Molecule Spectroscopy and Superresolution Imaging IX. Vol. 9714 SPIE, 2016.
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Requena, S, Raut, SL, Doan, H, Kimball, J, Fudala, R, Borejdo, J, Gryczynski, I, Strzhemechny, Y & Gryczynski, Z 2016, Multi-pulse pumping for far-field super-resolution imaging. in Single Molecule Spectroscopy and Superresolution Imaging IX. vol. 9714, 97140F, SPIE, Single Molecule Spectroscopy and Superresolution Imaging IX, San Francisco, United States, 13/02/16. https://doi.org/10.1117/12.2221921

Multi-pulse pumping for far-field super-resolution imaging. / Requena, Sebastian; Raut, Sangram Limbaji; Doan, Hung; Kimball, Joe; Fudala, Rafal; Borejdo, Julian; Gryczynski, Ignacy; Strzhemechny, Yuri; Gryczynski, Zygmunt.

Single Molecule Spectroscopy and Superresolution Imaging IX. Vol. 9714 SPIE, 2016. 97140F.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Requena S, Raut SL, Doan H, Kimball J, Fudala R, Borejdo J et al. Multi-pulse pumping for far-field super-resolution imaging. In Single Molecule Spectroscopy and Superresolution Imaging IX. Vol. 9714. SPIE. 2016. 97140F https://doi.org/10.1117/12.2221921