@inproceedings{fd3bb04b164545fcbbc09452eceb8668,
title = "Multi-pulse pumping for far-field super-resolution imaging",
abstract = "Recently, far-field optical imaging with a resolution significantly beyond diffraction limit has attracted tremendous attention allowing for high resolution imaging in living objects. Various methods have been proposed that are divided in to two basic approaches; deterministic super-resolution like STED or RESOLFT and stochastic super-resolution like PALM or STORM. We propose to achieve super-resolution in far-field fluorescence imaging by the use of controllable (on-demand) bursts of pulses that can change the fluorescence signal of long-lived component over one order of magnitude. We demonstrate that two beads, one labeled with a long-lived dye and another with a short-lived dye, separated by a distance lower than 100 nm can be easily resolved in a single experiment. The proposed method can be used to separate two biological structures in a cell by targeting them with two antibodies labeled with long-lived and short-lived fluorophores.",
keywords = "Super-resolution, microscopy, multi-pulse pumping",
author = "Sebastian Requena and Sangram Raut and Hung Doan and Joe Kimball and Rafal Fudala and Julian Borejdo and Ignacy Gryczynski and Yuri Strzhemechny and Zygmunt Gryczynski",
year = "2016",
month = jan,
day = "1",
doi = "10.1117/12.2221921",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Jorg Enderlein and Ingo Gregor and Gryczynski, {Zygmunt Karol} and Rainer Erdmann and Felix Koberling",
booktitle = "Single Molecule Spectroscopy and Superresolution Imaging IX",
note = "Single Molecule Spectroscopy and Superresolution Imaging IX ; Conference date: 13-02-2016 Through 14-02-2016",
}