Multi-photon excitation of fluorescence

Joseph R. Lakowicz, Ignacy Gryczynski

Research output: Contribution to journalConference articlepeer-review

3 Scopus citations


The increasing availability of fs lasers has resulted in expanded interest in multi-photon excitation of fluorescence. In this overview paper, we describe recent developments in multi-photon induced fluorescence spectroscopy and anisotropy. Experimental results are presented for three-photon excitation of tyrosine and tryptophan, and for the single tryptophan protein, troponin C Mutant F22. Three-photon excitation has been also applied to study DPH-labeled membranes and DNA stained with DAPI. The enhancement in spatial resolution resulting from three-photon excitation in spectroscopic and microscopic conditions is described for BBO scintillator in solution. We also describe two-photon excitation with using two-photon at different wavelengths.

Original languageEnglish
Pages (from-to)24-37
Number of pages14
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 1998
EventAdvances in Optical Biophysics - San Jose, CA, United States
Duration: 25 Jan 199826 Jan 1998


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