Multi-photon excitation of fluorescence

Joseph R. Lakowicz; Ignacy Gryczynski

doi:10.1117/12.307075

Multi-photon excitation of fluorescence

Joseph R. Lakowicz, Ignacy Gryczynski

Research output: Contribution to journal › Conference article

3 Scopus citations

Abstract

The increasing availability of fs lasers has resulted in expanded interest in multi-photon excitation of fluorescence. In this overview paper, we describe recent developments in multi-photon induced fluorescence spectroscopy and anisotropy. Experimental results are presented for three-photon excitation of tyrosine and tryptophan, and for the single tryptophan protein, troponin C Mutant F22. Three-photon excitation has been also applied to study DPH-labeled membranes and DNA stained with DAPI. The enhancement in spatial resolution resulting from three-photon excitation in spectroscopic and microscopic conditions is described for BBO scintillator in solution. We also describe two-photon excitation with using two-photon at different wavelengths.

Original language	English
Pages (from-to)	24-37
Number of pages	14
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3256
DOIs	https://doi.org/10.1117/12.307075
State	Published - 1 Dec 1998
Event	Advances in Optical Biophysics - San Jose, CA, United States Duration: 25 Jan 1998 → 26 Jan 1998

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Lakowicz, J. R., & Gryczynski, I. (1998). Multi-photon excitation of fluorescence. Proceedings of SPIE - The International Society for Optical Engineering, 3256, 24-37. https://doi.org/10.1117/12.307075

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AU - Gryczynski, Ignacy

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AB - The increasing availability of fs lasers has resulted in expanded interest in multi-photon excitation of fluorescence. In this overview paper, we describe recent developments in multi-photon induced fluorescence spectroscopy and anisotropy. Experimental results are presented for three-photon excitation of tyrosine and tryptophan, and for the single tryptophan protein, troponin C Mutant F22. Three-photon excitation has been also applied to study DPH-labeled membranes and DNA stained with DAPI. The enhancement in spatial resolution resulting from three-photon excitation in spectroscopic and microscopic conditions is described for BBO scintillator in solution. We also describe two-photon excitation with using two-photon at different wavelengths.

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