Multi-analyte multi-color enhanced fluorescent immunoassay on silver island-coated surface

Evgenia G. Matveeva; Zygmunt Gryczynski; Joseph R. Lakowicz

doi:10.1117/12.591685

Multi-analyte multi-color enhanced fluorescent immunoassay on silver island-coated surface

Evgenia G. Matveeva, Zygmunt Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journal › Conference article › peer-review

Abstract

A multi-analyte multi-color immunoassay utilizing enhanced fluorophores fluorescence in the proximity of silver island film (SIF) coated surface is described. A mixture of four different model analyte proteins was immobilized on the surface, and fluorescence signal of four corresponding antibodies labeled with different color fluorophores (emission maxima varied from 570 to 700 nm) was monitored using appropriate cut-off filters. To localize the excitation volume within close proximity of the SIF surface we used the total internal reflection (TIR) mode of excitation. Use of SIF coating resulted in approximately 5-10 fold increase in the intensity of the fluorophores signal if compared to the non-coated glass substrate surface. We suggest application of this methodology for multiplex immunoassays for simultaneous detection of several different analytes in medicine and biotechnology.

Original language	English
Article number	65
Pages (from-to)	366-374
Number of pages	9
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5692
DOIs	https://doi.org/10.1117/12.591685
State	Published - 21 Jul 2005
Event	Advanced Biomedical and Clinical Diagnostic Systems III - San Jose, CA, United States Duration: 23 Jan 2005 → 26 Jan 2005

Keywords

Enhanced fluorescence
Fluorescence immunoassay
Immunoglobulin
Multi-analyte
Multi-color
Nanosize metallic particles
Silver island films

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10.1117/12.591685

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title = "Multi-analyte multi-color enhanced fluorescent immunoassay on silver island-coated surface",

abstract = "A multi-analyte multi-color immunoassay utilizing enhanced fluorophores fluorescence in the proximity of silver island film (SIF) coated surface is described. A mixture of four different model analyte proteins was immobilized on the surface, and fluorescence signal of four corresponding antibodies labeled with different color fluorophores (emission maxima varied from 570 to 700 nm) was monitored using appropriate cut-off filters. To localize the excitation volume within close proximity of the SIF surface we used the total internal reflection (TIR) mode of excitation. Use of SIF coating resulted in approximately 5-10 fold increase in the intensity of the fluorophores signal if compared to the non-coated glass substrate surface. We suggest application of this methodology for multiplex immunoassays for simultaneous detection of several different analytes in medicine and biotechnology.",

keywords = "Enhanced fluorescence, Fluorescence immunoassay, Immunoglobulin, Multi-analyte, Multi-color, Nanosize metallic particles, Silver island films",

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T1 - Multi-analyte multi-color enhanced fluorescent immunoassay on silver island-coated surface

AU - Matveeva, Evgenia G.

AU - Gryczynski, Zygmunt

AU - Lakowicz, Joseph R.

PY - 2005/7/21

Y1 - 2005/7/21

N2 - A multi-analyte multi-color immunoassay utilizing enhanced fluorophores fluorescence in the proximity of silver island film (SIF) coated surface is described. A mixture of four different model analyte proteins was immobilized on the surface, and fluorescence signal of four corresponding antibodies labeled with different color fluorophores (emission maxima varied from 570 to 700 nm) was monitored using appropriate cut-off filters. To localize the excitation volume within close proximity of the SIF surface we used the total internal reflection (TIR) mode of excitation. Use of SIF coating resulted in approximately 5-10 fold increase in the intensity of the fluorophores signal if compared to the non-coated glass substrate surface. We suggest application of this methodology for multiplex immunoassays for simultaneous detection of several different analytes in medicine and biotechnology.

AB - A multi-analyte multi-color immunoassay utilizing enhanced fluorophores fluorescence in the proximity of silver island film (SIF) coated surface is described. A mixture of four different model analyte proteins was immobilized on the surface, and fluorescence signal of four corresponding antibodies labeled with different color fluorophores (emission maxima varied from 570 to 700 nm) was monitored using appropriate cut-off filters. To localize the excitation volume within close proximity of the SIF surface we used the total internal reflection (TIR) mode of excitation. Use of SIF coating resulted in approximately 5-10 fold increase in the intensity of the fluorophores signal if compared to the non-coated glass substrate surface. We suggest application of this methodology for multiplex immunoassays for simultaneous detection of several different analytes in medicine and biotechnology.

KW - Enhanced fluorescence

KW - Fluorescence immunoassay

KW - Immunoglobulin

KW - Multi-analyte

KW - Multi-color

KW - Nanosize metallic particles

KW - Silver island films

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U2 - 10.1117/12.591685

DO - 10.1117/12.591685

M3 - Conference article

AN - SCOPUS:21844446851

SN - 1605-7422

VL - 5692

SP - 366

EP - 374

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

M1 - 65

Y2 - 23 January 2005 through 26 January 2005

ER -

Multi-analyte multi-color enhanced fluorescent immunoassay on silver island-coated surface

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Keywords

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