Morphometric analysis of epithelial cells of frog urinary bladder. I. Effect of antidiuretic hormone, calcium ionophore (A23187) and PGE2

A. J. Mia, L. X. Oakford, L. Torres, C. Herman, Thomas Yorio

Research output: Contribution to journalArticle

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Abstract

Changes in epithelial cell morphology, especially at the apical plasma membrane, are frequently cited as initial evidence for antidiuretic hormone (ADH)-induced increase in membrane permeability. The effects of ADH and agents that alter and modify calcium and prostaglandin concentrations on the morphology and cytology of the epithelial cells of frog (Rana pipiens) urinary bladder are presented using the techniques of transmission and scanning electron microscopy. It was found that, like ADH, calcium ionophore, A23187, produce intense microvilli formation, microfilament mobilization and an increase in the density of granules and membrane associated vesicles, suggesting a prominent role of calcium in these processes. Moreover, our results suggest that these membrane and cytosolic transformations may be mediated in part through prostaglandin formation, as exogenous PGE2 mimicked these effects, and indomethacin, a prostaglandin synthesis inhibitor, attenuated ionophore's effect on luminal cytomorphology. However, unlike ADH, prostaglandins and ionophore inhibit hormonal-induced increase in transepithelial water flow. These results suggest that other components more distal to the luminal membrane, perhaps the basolateral membrane, may be rate-limiting for transepithelial water flow and possibly are regulated by either changes in calcium concentrations or prostaglandins.

Original languageEnglish
Pages (from-to)437-450
Number of pages14
JournalTissue and Cell
Volume19
Issue number3
DOIs
StatePublished - 1 Jan 1987

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Calcium Ionophores
Calcimycin
Vasopressins
Dinoprostone
Anura
Urinary Bladder
Epithelial Cells
Prostaglandins
Membranes
Ionophores
Calcium
Antidiuretic Agents
Rana pipiens
Prostaglandin Antagonists
Scanning Transmission Electron Microscopy
Water
Microvilli
Actin Cytoskeleton
Indomethacin
Cell Biology

Keywords

  • ADH
  • calcium
  • ionophore
  • morphology
  • prostaglandins

Cite this

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title = "Morphometric analysis of epithelial cells of frog urinary bladder. I. Effect of antidiuretic hormone, calcium ionophore (A23187) and PGE2",
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Morphometric analysis of epithelial cells of frog urinary bladder. I. Effect of antidiuretic hormone, calcium ionophore (A23187) and PGE2. / Mia, A. J.; Oakford, L. X.; Torres, L.; Herman, C.; Yorio, Thomas.

In: Tissue and Cell, Vol. 19, No. 3, 01.01.1987, p. 437-450.

Research output: Contribution to journalArticle

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