TY - JOUR
T1 - Morphological changes of supported lipid bilayers induced by lysozyme
T2 - Planar domain formation vs. multilayer stacking
AU - Trusova, Valeriya M.
AU - Gorbenko, Galyna P.
AU - Akopova, Irina
AU - Molotkovsky, Julian G.
AU - Gryczynski, Ignacy
AU - Borejdo, Julian
AU - Gryczynski, Zygmunt
N1 - Funding Information:
This work was supported by the grant no. 4534 from the Science and Technology Center in Ukraine, grant no. F28.4/007 from Fundamental Research State Fund , grant NIH EB012334 (to Z.G.), grant from European Social Fund (project number 2009/0205/1DP/1.1.1.2.0/09/APIA/VIAA/152) and grant 09-04-00313 of Russian Foundation for Basic Research . VT gratefully acknowledges an award by Human Frontier Science Program.
PY - 2010/10
Y1 - 2010/10
N2 - Total internal reflection fluorescence microscopy (TIRFM) has been utilized to explore the effect of cationic protein lysozyme (Lz) on the morphology of solid-supported lipid bilayers (SLBs) comprised of zwitterionic lipid phosphatidylcholine (PC) and its mixture with anionic lipid cardiolipin (CL). Kinetic TIRFM imaging of different systems revealed subtle interplay between lipid lateral segregation accompanied by exchange of neutral and acidic lipids in the protein-lipid interaction zone, and the formation of lipid multilayer stacks. The switch between these states was shown to be controlled by CL content. In weakly charged SLBs containing 5. mol% CL, assembling of CL molecules into planar domains upon Lz adsorption has been observed while at higher content of anionic lipid (25. mol%) in-plane domains tend to transform into multilayer stacks, thereby ensuring the most thermodynamically-favorable membrane conformation.
AB - Total internal reflection fluorescence microscopy (TIRFM) has been utilized to explore the effect of cationic protein lysozyme (Lz) on the morphology of solid-supported lipid bilayers (SLBs) comprised of zwitterionic lipid phosphatidylcholine (PC) and its mixture with anionic lipid cardiolipin (CL). Kinetic TIRFM imaging of different systems revealed subtle interplay between lipid lateral segregation accompanied by exchange of neutral and acidic lipids in the protein-lipid interaction zone, and the formation of lipid multilayer stacks. The switch between these states was shown to be controlled by CL content. In weakly charged SLBs containing 5. mol% CL, assembling of CL molecules into planar domains upon Lz adsorption has been observed while at higher content of anionic lipid (25. mol%) in-plane domains tend to transform into multilayer stacks, thereby ensuring the most thermodynamically-favorable membrane conformation.
KW - Lipid demixing
KW - Lysozyme
KW - Multilayer stacks
KW - Supported lipid bilayers
UR - http://www.scopus.com/inward/record.url?scp=77955051857&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2010.06.011
DO - 10.1016/j.colsurfb.2010.06.011
M3 - Article
C2 - 20620034
AN - SCOPUS:77955051857
SN - 0927-7765
VL - 80
SP - 219
EP - 226
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 2
ER -