Morphological changes of supported lipid bilayers induced by lysozyme: Planar domain formation vs. multilayer stacking

Valeriya M. Trusova, Galyna P. Gorbenko, Irina Akopova, Julian G. Molotkovsky, Ignacy Gryczynski, Julian Borejdo, Zygmunt Gryczynski

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Total internal reflection fluorescence microscopy (TIRFM) has been utilized to explore the effect of cationic protein lysozyme (Lz) on the morphology of solid-supported lipid bilayers (SLBs) comprised of zwitterionic lipid phosphatidylcholine (PC) and its mixture with anionic lipid cardiolipin (CL). Kinetic TIRFM imaging of different systems revealed subtle interplay between lipid lateral segregation accompanied by exchange of neutral and acidic lipids in the protein-lipid interaction zone, and the formation of lipid multilayer stacks. The switch between these states was shown to be controlled by CL content. In weakly charged SLBs containing 5. mol% CL, assembling of CL molecules into planar domains upon Lz adsorption has been observed while at higher content of anionic lipid (25. mol%) in-plane domains tend to transform into multilayer stacks, thereby ensuring the most thermodynamically-favorable membrane conformation.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume80
Issue number2
DOIs
StatePublished - Oct 2010

Keywords

  • Lipid demixing
  • Lysozyme
  • Multilayer stacks
  • Supported lipid bilayers

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