Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique

Wieslaw I. Gruszecki, Rafal Luchowski, Piotr Wasko, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Amphotericin B (AmB) is a polyene antibiotic used to treat deep-seated mycoses. Both the pharmaceutical and toxic activities of AmB depend on the molecular organization of the drug. The fluorescence of AmB has proven to be a powerful technique of studying the drug's association state. In particular, fluorescence lifetime appeared to be sensitive to the formation of AmB dimers and aggregated structures. This paper addresses the application of the fluorescence technique in the study of the molecular organization of AmB, and perspectives on future application of this approach are addressed briefly.

Original languageEnglish
Title of host publicationSpectroscopic Methods of Analysis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages57-65
Number of pages9
ISBN (Print)9781617798054
DOIs
StatePublished - 1 Jan 2012

Publication series

NameMethods in Molecular Biology
Volume875
ISSN (Print)1064-3745

Keywords

  • Amphotericin B
  • FLIM
  • Fluorescence
  • Fluorescence lifetime
  • Polyene antibiotic

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    Gruszecki, W. I., Luchowski, R., Wasko, P., Gryczynski, Z., & Gryczynski, I. (2012). Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique. In Spectroscopic Methods of Analysis: Methods and Protocols (pp. 57-65). (Methods in Molecular Biology; Vol. 875). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-806-1_3