Abstract
Amphotericin B (AmB) is a polyene antibiotic used to treat deep-seated mycoses. Both the pharmaceutical and toxic activities of AmB depend on the molecular organization of the drug. The fluorescence of AmB has proven to be a powerful technique of studying the drug's association state. In particular, fluorescence lifetime appeared to be sensitive to the formation of AmB dimers and aggregated structures. This paper addresses the application of the fluorescence technique in the study of the molecular organization of AmB, and perspectives on future application of this approach are addressed briefly.
| Original language | English |
|---|---|
| Title of host publication | Spectroscopic Methods of Analysis |
| Subtitle of host publication | Methods and Protocols |
| Publisher | Humana Press Inc. |
| Pages | 57-65 |
| Number of pages | 9 |
| ISBN (Print) | 9781617798054 |
| DOIs | |
| State | Published - 1 Jan 2012 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 875 |
| ISSN (Print) | 1064-3745 |
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Keywords
- Amphotericin B
- FLIM
- Fluorescence
- Fluorescence lifetime
- Polyene antibiotic
Cite this
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Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique. / Gruszecki, Wieslaw I.; Luchowski, Rafal; Wasko, Piotr; Gryczynski, Zygmunt; Gryczynski, Ignacy.
Spectroscopic Methods of Analysis: Methods and Protocols. Humana Press Inc., 2012. p. 57-65 (Methods in Molecular Biology; Vol. 875).Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique
AU - Gruszecki, Wieslaw I.
AU - Luchowski, Rafal
AU - Wasko, Piotr
AU - Gryczynski, Zygmunt
AU - Gryczynski, Ignacy
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Amphotericin B (AmB) is a polyene antibiotic used to treat deep-seated mycoses. Both the pharmaceutical and toxic activities of AmB depend on the molecular organization of the drug. The fluorescence of AmB has proven to be a powerful technique of studying the drug's association state. In particular, fluorescence lifetime appeared to be sensitive to the formation of AmB dimers and aggregated structures. This paper addresses the application of the fluorescence technique in the study of the molecular organization of AmB, and perspectives on future application of this approach are addressed briefly.
AB - Amphotericin B (AmB) is a polyene antibiotic used to treat deep-seated mycoses. Both the pharmaceutical and toxic activities of AmB depend on the molecular organization of the drug. The fluorescence of AmB has proven to be a powerful technique of studying the drug's association state. In particular, fluorescence lifetime appeared to be sensitive to the formation of AmB dimers and aggregated structures. This paper addresses the application of the fluorescence technique in the study of the molecular organization of AmB, and perspectives on future application of this approach are addressed briefly.
KW - Amphotericin B
KW - FLIM
KW - Fluorescence
KW - Fluorescence lifetime
KW - Polyene antibiotic
UR - http://www.scopus.com/inward/record.url?scp=84865815320&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-806-1_3
DO - 10.1007/978-1-61779-806-1_3
M3 - Chapter
C2 - 22573435
AN - SCOPUS:84865815320
SN - 9781617798054
T3 - Methods in Molecular Biology
SP - 57
EP - 65
BT - Spectroscopic Methods of Analysis
PB - Humana Press Inc.
ER -