Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique

Wieslaw I. Gruszecki; Rafal Luchowski; Piotr Wasko; Zygmunt Gryczynski; Ignacy Gryczynski

doi:10.1007/978-1-61779-806-1_3

Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique

Wieslaw I. Gruszecki, Rafal Luchowski, Piotr Wasko, Zygmunt Gryczynski, Ignacy Gryczynski

Microbiology, Immunology & Genetics

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

Amphotericin B (AmB) is a polyene antibiotic used to treat deep-seated mycoses. Both the pharmaceutical and toxic activities of AmB depend on the molecular organization of the drug. The fluorescence of AmB has proven to be a powerful technique of studying the drug's association state. In particular, fluorescence lifetime appeared to be sensitive to the formation of AmB dimers and aggregated structures. This paper addresses the application of the fluorescence technique in the study of the molecular organization of AmB, and perspectives on future application of this approach are addressed briefly.

Original language	English
Title of host publication	Spectroscopic Methods of Analysis
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	57-65
Number of pages	9
ISBN (Print)	9781617798054
DOIs	https://doi.org/10.1007/978-1-61779-806-1_3
State	Published - 1 Jan 2012

Publication series

Name	Methods in Molecular Biology
Volume	875
ISSN (Print)	1064-3745

Keywords

Amphotericin B
FLIM
Fluorescence
Fluorescence lifetime
Polyene antibiotic

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10.1007/978-1-61779-806-1_3

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abstract = "Amphotericin B (AmB) is a polyene antibiotic used to treat deep-seated mycoses. Both the pharmaceutical and toxic activities of AmB depend on the molecular organization of the drug. The fluorescence of AmB has proven to be a powerful technique of studying the drug's association state. In particular, fluorescence lifetime appeared to be sensitive to the formation of AmB dimers and aggregated structures. This paper addresses the application of the fluorescence technique in the study of the molecular organization of AmB, and perspectives on future application of this approach are addressed briefly.",

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Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique. / Gruszecki, Wieslaw I.; Luchowski, Rafal; Wasko, Piotr; Gryczynski, Zygmunt; Gryczynski, Ignacy.

Spectroscopic Methods of Analysis: Methods and Protocols. Humana Press Inc., 2012. p. 57-65 (Methods in Molecular Biology; Vol. 875).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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T1 - Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique

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AU - Wasko, Piotr

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

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AB - Amphotericin B (AmB) is a polyene antibiotic used to treat deep-seated mycoses. Both the pharmaceutical and toxic activities of AmB depend on the molecular organization of the drug. The fluorescence of AmB has proven to be a powerful technique of studying the drug's association state. In particular, fluorescence lifetime appeared to be sensitive to the formation of AmB dimers and aggregated structures. This paper addresses the application of the fluorescence technique in the study of the molecular organization of AmB, and perspectives on future application of this approach are addressed briefly.

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KW - FLIM

KW - Fluorescence

KW - Fluorescence lifetime

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Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique

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