Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique

Wieslaw I. Gruszecki, Rafal Luchowski, Piotr Wasko, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Amphotericin B (AmB) is a polyene antibiotic used to treat deep-seated mycoses. Both the pharmaceutical and toxic activities of AmB depend on the molecular organization of the drug. The fluorescence of AmB has proven to be a powerful technique of studying the drug's association state. In particular, fluorescence lifetime appeared to be sensitive to the formation of AmB dimers and aggregated structures. This paper addresses the application of the fluorescence technique in the study of the molecular organization of AmB, and perspectives on future application of this approach are addressed briefly.

Original languageEnglish
Title of host publicationSpectroscopic Methods of Analysis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages57-65
Number of pages9
ISBN (Print)9781617798054
DOIs
StatePublished - 1 Jan 2012

Publication series

NameMethods in Molecular Biology
Volume875
ISSN (Print)1064-3745

Fingerprint

Polyenes
Amphotericin B
Fluorescence
Anti-Bacterial Agents
Pharmaceutical Preparations
Mycoses
Poisons

Keywords

  • Amphotericin B
  • FLIM
  • Fluorescence
  • Fluorescence lifetime
  • Polyene antibiotic

Cite this

Gruszecki, W. I., Luchowski, R., Wasko, P., Gryczynski, Z., & Gryczynski, I. (2012). Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique. In Spectroscopic Methods of Analysis: Methods and Protocols (pp. 57-65). (Methods in Molecular Biology; Vol. 875). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-806-1_3
Gruszecki, Wieslaw I. ; Luchowski, Rafal ; Wasko, Piotr ; Gryczynski, Zygmunt ; Gryczynski, Ignacy. / Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique. Spectroscopic Methods of Analysis: Methods and Protocols. Humana Press Inc., 2012. pp. 57-65 (Methods in Molecular Biology).
@inbook{88a4452660e649398416f7d30a9f3544,
title = "Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique",
abstract = "Amphotericin B (AmB) is a polyene antibiotic used to treat deep-seated mycoses. Both the pharmaceutical and toxic activities of AmB depend on the molecular organization of the drug. The fluorescence of AmB has proven to be a powerful technique of studying the drug's association state. In particular, fluorescence lifetime appeared to be sensitive to the formation of AmB dimers and aggregated structures. This paper addresses the application of the fluorescence technique in the study of the molecular organization of AmB, and perspectives on future application of this approach are addressed briefly.",
keywords = "Amphotericin B, FLIM, Fluorescence, Fluorescence lifetime, Polyene antibiotic",
author = "Gruszecki, {Wieslaw I.} and Rafal Luchowski and Piotr Wasko and Zygmunt Gryczynski and Ignacy Gryczynski",
year = "2012",
month = "1",
day = "1",
doi = "10.1007/978-1-61779-806-1_3",
language = "English",
isbn = "9781617798054",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "57--65",
booktitle = "Spectroscopic Methods of Analysis",

}

Gruszecki, WI, Luchowski, R, Wasko, P, Gryczynski, Z & Gryczynski, I 2012, Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique. in Spectroscopic Methods of Analysis: Methods and Protocols. Methods in Molecular Biology, vol. 875, Humana Press Inc., pp. 57-65. https://doi.org/10.1007/978-1-61779-806-1_3

Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique. / Gruszecki, Wieslaw I.; Luchowski, Rafal; Wasko, Piotr; Gryczynski, Zygmunt; Gryczynski, Ignacy.

Spectroscopic Methods of Analysis: Methods and Protocols. Humana Press Inc., 2012. p. 57-65 (Methods in Molecular Biology; Vol. 875).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique

AU - Gruszecki, Wieslaw I.

AU - Luchowski, Rafal

AU - Wasko, Piotr

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Amphotericin B (AmB) is a polyene antibiotic used to treat deep-seated mycoses. Both the pharmaceutical and toxic activities of AmB depend on the molecular organization of the drug. The fluorescence of AmB has proven to be a powerful technique of studying the drug's association state. In particular, fluorescence lifetime appeared to be sensitive to the formation of AmB dimers and aggregated structures. This paper addresses the application of the fluorescence technique in the study of the molecular organization of AmB, and perspectives on future application of this approach are addressed briefly.

AB - Amphotericin B (AmB) is a polyene antibiotic used to treat deep-seated mycoses. Both the pharmaceutical and toxic activities of AmB depend on the molecular organization of the drug. The fluorescence of AmB has proven to be a powerful technique of studying the drug's association state. In particular, fluorescence lifetime appeared to be sensitive to the formation of AmB dimers and aggregated structures. This paper addresses the application of the fluorescence technique in the study of the molecular organization of AmB, and perspectives on future application of this approach are addressed briefly.

KW - Amphotericin B

KW - FLIM

KW - Fluorescence

KW - Fluorescence lifetime

KW - Polyene antibiotic

UR - http://www.scopus.com/inward/record.url?scp=84865815320&partnerID=8YFLogxK

U2 - 10.1007/978-1-61779-806-1_3

DO - 10.1007/978-1-61779-806-1_3

M3 - Chapter

C2 - 22573435

AN - SCOPUS:84865815320

SN - 9781617798054

T3 - Methods in Molecular Biology

SP - 57

EP - 65

BT - Spectroscopic Methods of Analysis

PB - Humana Press Inc.

ER -

Gruszecki WI, Luchowski R, Wasko P, Gryczynski Z, Gryczynski I. Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique. In Spectroscopic Methods of Analysis: Methods and Protocols. Humana Press Inc. 2012. p. 57-65. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-806-1_3