TY - JOUR
T1 - Molecular fluorescence enhancement on fractal-like structures
AU - Luchowski, Rafal
AU - Shtoyko, Tanya
AU - Matveeva, Evgenia
AU - Sarkar, Pabak
AU - Borejdo, Julian
AU - Gryczynski, Zygmunt
AU - Gryczynski, Ignacy
PY - 2010/6
Y1 - 2010/6
N2 - In this report we discuss strong fluorescence enhancements on electrochemically grown silver nanostructures examined through fluorescence lifetime imaging microscopy (FLIM). Silver fractal-like nanostructures were deposited on three different substrates: glass, plastic, and silicon. For all of the surfaces the same dye was tested, Dj Light 649, deposited in the form of a model immunoassay through excitation from a 635 nm pulsed solid-state laser. The brightness improvement in hot spots exceeded 300 fold, which is about two times higher than was observed previously on similar surfaces. The strongest enhancements correspond to the shortest lifetimes, indicating a strong interaction between excited molecules and silver nanostructures. Additionally, the photostability of the fluorescence dye was dramatically increased in the presence of electrochemically deposited silver nanostructures. The production of silver fractals is easy, very controllable, and can be applied to any surface. We therefore believe that silver fractal-like nanostructures can be used successfully in ultrasensitive assays and fluorophore trace detection.
AB - In this report we discuss strong fluorescence enhancements on electrochemically grown silver nanostructures examined through fluorescence lifetime imaging microscopy (FLIM). Silver fractal-like nanostructures were deposited on three different substrates: glass, plastic, and silicon. For all of the surfaces the same dye was tested, Dj Light 649, deposited in the form of a model immunoassay through excitation from a 635 nm pulsed solid-state laser. The brightness improvement in hot spots exceeded 300 fold, which is about two times higher than was observed previously on similar surfaces. The strongest enhancements correspond to the shortest lifetimes, indicating a strong interaction between excited molecules and silver nanostructures. Additionally, the photostability of the fluorescence dye was dramatically increased in the presence of electrochemically deposited silver nanostructures. The production of silver fractals is easy, very controllable, and can be applied to any surface. We therefore believe that silver fractal-like nanostructures can be used successfully in ultrasensitive assays and fluorophore trace detection.
KW - Fluorescence microscopy
KW - Fractallike structures
KW - High sensitivity detection
KW - Nanostructures
KW - Surface plasmonics
UR - http://www.scopus.com/inward/record.url?scp=77953814948&partnerID=8YFLogxK
U2 - 10.1366/000370210791414317
DO - 10.1366/000370210791414317
M3 - Article
C2 - 20537224
AN - SCOPUS:77953814948
SN - 0003-7028
VL - 64
SP - 578
EP - 583
JO - Applied Spectroscopy
JF - Applied Spectroscopy
IS - 6
ER -