Molecular determinants mediating gating of transient receptor potential canonical (TRPC) channels by stromal interaction molecule 1 (STIM1)

Kyu Pil Lee, Seok Choi, Jeong Hee Hong, Malini Ahuja, Sarabeth Graham, Rong Ma, Insuk So, Dong Min Shin, Shmuel Muallem, Joseph P. Yuan

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Abstract

Background: STIM1 gates TRPC channels, but the interacting domains are unknown. Results: The TRPC N and C terminus coiled coil domains interact to restrict access of STIM1. Their dissociation by cell stimulation promotes STIM1 interaction. Conclusion: The STIM1 Orai1-activating region (SOAR) domain interacts with the TRPC C terminus CCD to open the channels. Significance: The findings reveal how STIM1 opens the TRPC channels to control receptor-stimulated Ca2+ influx.

Original languageEnglish
Pages (from-to)6372-6382
Number of pages11
JournalJournal of Biological Chemistry
Volume289
Issue number10
DOIs
StatePublished - 7 Mar 2014

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Transient Receptor Potential Channels
Molecules
Charge coupled devices
Stromal Interaction Molecule 1

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Lee, Kyu Pil ; Choi, Seok ; Hong, Jeong Hee ; Ahuja, Malini ; Graham, Sarabeth ; Ma, Rong ; So, Insuk ; Shin, Dong Min ; Muallem, Shmuel ; Yuan, Joseph P. / Molecular determinants mediating gating of transient receptor potential canonical (TRPC) channels by stromal interaction molecule 1 (STIM1). In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 10. pp. 6372-6382.
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Molecular determinants mediating gating of transient receptor potential canonical (TRPC) channels by stromal interaction molecule 1 (STIM1). / Lee, Kyu Pil; Choi, Seok; Hong, Jeong Hee; Ahuja, Malini; Graham, Sarabeth; Ma, Rong; So, Insuk; Shin, Dong Min; Muallem, Shmuel; Yuan, Joseph P.

In: Journal of Biological Chemistry, Vol. 289, No. 10, 07.03.2014, p. 6372-6382.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Lee, Kyu Pil

AU - Choi, Seok

AU - Hong, Jeong Hee

AU - Ahuja, Malini

AU - Graham, Sarabeth

AU - Ma, Rong

AU - So, Insuk

AU - Shin, Dong Min

AU - Muallem, Shmuel

AU - Yuan, Joseph P.

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