Molecular determinants mediating gating of transient receptor potential canonical (TRPC) channels by stromal interaction molecule 1 (STIM1)

Kyu Pil Lee; Seok Choi; Jeong Hee Hong; Malini Ahuja; Sarabeth Graham; Rong Ma; Insuk So; Dong Min Shin; Shmuel Muallem; Joseph P. Yuan

doi:10.1074/jbc.M113.546556

Molecular determinants mediating gating of transient receptor potential canonical (TRPC) channels by stromal interaction molecule 1 (STIM1)

Kyu Pil Lee, Seok Choi, Jeong Hee Hong, Malini Ahuja, Sarabeth Graham, Rong Ma, Insuk So, Dong Min Shin, Shmuel Muallem, Joseph P. Yuan

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

Background: STIM1 gates TRPC channels, but the interacting domains are unknown. Results: The TRPC N and C terminus coiled coil domains interact to restrict access of STIM1. Their dissociation by cell stimulation promotes STIM1 interaction. Conclusion: The STIM1 Orai1-activating region (SOAR) domain interacts with the TRPC C terminus CCD to open the channels. Significance: The findings reveal how STIM1 opens the TRPC channels to control receptor-stimulated Ca²⁺ influx.

Original language	English
Pages (from-to)	6372-6382
Number of pages	11
Journal	Journal of Biological Chemistry
Volume	289
Issue number	10
DOIs	https://doi.org/10.1074/jbc.M113.546556
State	Published - 7 Mar 2014

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title = "Molecular determinants mediating gating of transient receptor potential canonical (TRPC) channels by stromal interaction molecule 1 (STIM1)",

abstract = "Background: STIM1 gates TRPC channels, but the interacting domains are unknown. Results: The TRPC N and C terminus coiled coil domains interact to restrict access of STIM1. Their dissociation by cell stimulation promotes STIM1 interaction. Conclusion: The STIM1 Orai1-activating region (SOAR) domain interacts with the TRPC C terminus CCD to open the channels. Significance: The findings reveal how STIM1 opens the TRPC channels to control receptor-stimulated Ca2+ influx.",

author = "Lee, {Kyu Pil} and Seok Choi and Hong, {Jeong Hee} and Malini Ahuja and Sarabeth Graham and Rong Ma and Insuk So and Shin, {Dong Min} and Shmuel Muallem and Yuan, {Joseph P.}",

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T1 - Molecular determinants mediating gating of transient receptor potential canonical (TRPC) channels by stromal interaction molecule 1 (STIM1)

AU - Lee, Kyu Pil

AU - Choi, Seok

AU - Hong, Jeong Hee

AU - Ahuja, Malini

AU - Graham, Sarabeth

AU - Ma, Rong

AU - So, Insuk

AU - Shin, Dong Min

AU - Muallem, Shmuel

AU - Yuan, Joseph P.

PY - 2014/3/7

Y1 - 2014/3/7

N2 - Background: STIM1 gates TRPC channels, but the interacting domains are unknown. Results: The TRPC N and C terminus coiled coil domains interact to restrict access of STIM1. Their dissociation by cell stimulation promotes STIM1 interaction. Conclusion: The STIM1 Orai1-activating region (SOAR) domain interacts with the TRPC C terminus CCD to open the channels. Significance: The findings reveal how STIM1 opens the TRPC channels to control receptor-stimulated Ca2+ influx.

AB - Background: STIM1 gates TRPC channels, but the interacting domains are unknown. Results: The TRPC N and C terminus coiled coil domains interact to restrict access of STIM1. Their dissociation by cell stimulation promotes STIM1 interaction. Conclusion: The STIM1 Orai1-activating region (SOAR) domain interacts with the TRPC C terminus CCD to open the channels. Significance: The findings reveal how STIM1 opens the TRPC channels to control receptor-stimulated Ca2+ influx.

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SN - 0021-9258

VL - 289

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EP - 6382

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 10

ER -

Molecular determinants mediating gating of transient receptor potential canonical (TRPC) channels by stromal interaction molecule 1 (STIM1)

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