TY - JOUR
T1 - Molecular cloning of LSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE)
AU - Matsuyama, Toshifumi
AU - Grossman, Alex
AU - Mittrücker, Hans willi
AU - Siderovski, David P.
AU - Kiefer, Friedemann
AU - Kawakami, Takatoshi
AU - Richardson, Christopher D.
AU - Taniguchi, Tadatsugu
AU - Yoshinaga, Steven K.
AU - Mak, Tak W.
N1 - Funding Information:
We thank A. Janssen, D. Ingrande, L. Arbogast and S. Suggs for DNA sequencing; B. Marshall and J. Mayer for peptide synthesis; B. Goodman for oligonucleotide synthesis; M. Rihanek for anti-serum production; L. Selander for RT-PCR data; A. Ho for computational work and R. Amakawa, T. Kimura, M. Kitagawa and V. Stambolic for suggestions and critical reviewing of the manuscript H.W.M. is supported by a grant of the Deutsche Forschungsgemeinschaft. This work was supported by grants from the Medical Research Council of Canada, the National Institute of Canada and the National Science and Engineering Research Council of Canada.
PY - 1995/6/25
Y1 - 1995/6/25
N2 - Interferon regulatory factor (IRF) genes encode a family of DNA-blndlng proteins that are involved in the transcriptional regulation of type-l Interferon and/or interferon-inducible genes. We report here the characterization of LSIRF, a new member of the IRF gene family cloned from mouse spleen by the polymerase chain reaction using degenerate primers. LSIRF was found to encode a 51 kDa protein that shares a high degree of amino acid sequence homology in the DNA-binding domain with other IRF family members. LSIRF expression was detectable only In lymphold cells. In contrast to other IRF genes, LSIRF expresslon was not Induced by interferons, but rather by antigen-receptor mediated stimuli such as plant lectins, CD3 or IgM crosslinking. In In vitro DNA binding studies, LSIRF was able to bind to the interferon-stimulated response element (ISRE) of the MHC class I promoter. The expression pattern and DNA binding activities suggest that LSIRF plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
AB - Interferon regulatory factor (IRF) genes encode a family of DNA-blndlng proteins that are involved in the transcriptional regulation of type-l Interferon and/or interferon-inducible genes. We report here the characterization of LSIRF, a new member of the IRF gene family cloned from mouse spleen by the polymerase chain reaction using degenerate primers. LSIRF was found to encode a 51 kDa protein that shares a high degree of amino acid sequence homology in the DNA-binding domain with other IRF family members. LSIRF expression was detectable only In lymphold cells. In contrast to other IRF genes, LSIRF expresslon was not Induced by interferons, but rather by antigen-receptor mediated stimuli such as plant lectins, CD3 or IgM crosslinking. In In vitro DNA binding studies, LSIRF was able to bind to the interferon-stimulated response element (ISRE) of the MHC class I promoter. The expression pattern and DNA binding activities suggest that LSIRF plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
UR - http://www.scopus.com/inward/record.url?scp=0029069958&partnerID=8YFLogxK
U2 - 10.1093/nar/23.12.2127
DO - 10.1093/nar/23.12.2127
M3 - Article
C2 - 7541907
AN - SCOPUS:0029069958
SN - 0305-1048
VL - 23
SP - 2127
EP - 2136
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 12
ER -