Molecular cloning of LSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE)

Toshifumi Matsuyama, Alex Grossman, Hans willi Mittrücker, David P. Siderovski, Friedemann Kiefer, Takatoshi Kawakami, Christopher D. Richardson, Tadatsugu Taniguchi, Steven K. Yoshinaga, Tak W. Mak

Research output: Contribution to journalArticlepeer-review

200 Scopus citations

Abstract

Interferon regulatory factor (IRF) genes encode a family of DNA-blndlng proteins that are involved in the transcriptional regulation of type-l Interferon and/or interferon-inducible genes. We report here the characterization of LSIRF, a new member of the IRF gene family cloned from mouse spleen by the polymerase chain reaction using degenerate primers. LSIRF was found to encode a 51 kDa protein that shares a high degree of amino acid sequence homology in the DNA-binding domain with other IRF family members. LSIRF expression was detectable only In lymphold cells. In contrast to other IRF genes, LSIRF expresslon was not Induced by interferons, but rather by antigen-receptor mediated stimuli such as plant lectins, CD3 or IgM crosslinking. In In vitro DNA binding studies, LSIRF was able to bind to the interferon-stimulated response element (ISRE) of the MHC class I promoter. The expression pattern and DNA binding activities suggest that LSIRF plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Original languageEnglish
Pages (from-to)2127-2136
Number of pages10
JournalNucleic Acids Research
Volume23
Issue number12
DOIs
StatePublished - 25 Jun 1995

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