Molecular cloning and expression of rat kallistatin gene

Karl X. Chai; Vincent C. Chen; Aiguo Ni; Klaus Lindpaintner; Speranza Rubattu; Lee Chao; Julie Chao

doi:10.1016/S0167-4781(97)00100-0

Molecular cloning and expression of rat kallistatin gene

Karl X. Chai, Vincent C. Chen, Aiguo Ni, Klaus Lindpaintner, Speranza Rubattu, Lee Chao, Julie Chao

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9 Scopus citations

Abstract

We have previously purified and cloned human kallistatin and rat kallikrein-binding protein (RKBP), which are tissue kallikrein inhibitors belonging to the serine proteinase inhibitor superfamily. In this study, we have cloned and sequenced the gene encoding rat kallistatin with Phe-Phe- Ser-Ala-Gln at positions P2-P3', which is identical to the reactive center of human kallistatin. Rat kallistatin is highly similar to human kallistatin, sharing 68% and 57% sequence identity at the cDNA and the amino acid levels. The rat kallistatin gene exists in a single copy and is located on chromosome 6. An SphI RFLP is found between SHR and WKY rats at or near the rat kallistatin gene locus. Two amino acid polymorphisms of the rat kallistatin gene between these two strains were found by sequence analysis. A candidate promoter in the 5'-flanking region (109 bp) of the rat kallistatin gene has been identified by reporter assays. The expression of rat kallistatin in the liver is growth-dependent and down-regulated during acute phase inflammation. Recombinant rat kallistatin produced in E. coli is able to bind to tissue kallikrein, and the interaction is inhibited by heparin. These characteristics define rat kallistatin as the counterpart of human kallistatin.

Original language	English
Pages (from-to)	277-286
Number of pages	10
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1353
Issue number	3
DOIs	https://doi.org/10.1016/S0167-4781(97)00100-0
State	Published - 12 Sep 1997

Keywords

(Rat)
Gene cloning
Kallikrein-binding protein
Kallistatin
Promoter activity
Recombinant protein
Serpin

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10.1016/S0167-4781(97)00100-0

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@article{e9075db3a8f24e87839f77a50877a22c,

title = "Molecular cloning and expression of rat kallistatin gene",

abstract = "We have previously purified and cloned human kallistatin and rat kallikrein-binding protein (RKBP), which are tissue kallikrein inhibitors belonging to the serine proteinase inhibitor superfamily. In this study, we have cloned and sequenced the gene encoding rat kallistatin with Phe-Phe- Ser-Ala-Gln at positions P2-P3', which is identical to the reactive center of human kallistatin. Rat kallistatin is highly similar to human kallistatin, sharing 68% and 57% sequence identity at the cDNA and the amino acid levels. The rat kallistatin gene exists in a single copy and is located on chromosome 6. An SphI RFLP is found between SHR and WKY rats at or near the rat kallistatin gene locus. Two amino acid polymorphisms of the rat kallistatin gene between these two strains were found by sequence analysis. A candidate promoter in the 5'-flanking region (109 bp) of the rat kallistatin gene has been identified by reporter assays. The expression of rat kallistatin in the liver is growth-dependent and down-regulated during acute phase inflammation. Recombinant rat kallistatin produced in E. coli is able to bind to tissue kallikrein, and the interaction is inhibited by heparin. These characteristics define rat kallistatin as the counterpart of human kallistatin.",

keywords = "(Rat), Gene cloning, Kallikrein-binding protein, Kallistatin, Promoter activity, Recombinant protein, Serpin",

author = "Chai, {Karl X.} and Chen, {Vincent C.} and Aiguo Ni and Klaus Lindpaintner and Speranza Rubattu and Lee Chao and Julie Chao",

note = "Funding Information: This work was supported by National Institutes of Health Grant HL44083. The authors thank Dr. Gary P. Richards for the critical reading of the manuscript.",

year = "1997",

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doi = "10.1016/S0167-4781(97)00100-0",

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T1 - Molecular cloning and expression of rat kallistatin gene

AU - Chai, Karl X.

AU - Chen, Vincent C.

AU - Ni, Aiguo

AU - Lindpaintner, Klaus

AU - Rubattu, Speranza

AU - Chao, Lee

AU - Chao, Julie

N1 - Funding Information: This work was supported by National Institutes of Health Grant HL44083. The authors thank Dr. Gary P. Richards for the critical reading of the manuscript.

PY - 1997/9/12

Y1 - 1997/9/12

N2 - We have previously purified and cloned human kallistatin and rat kallikrein-binding protein (RKBP), which are tissue kallikrein inhibitors belonging to the serine proteinase inhibitor superfamily. In this study, we have cloned and sequenced the gene encoding rat kallistatin with Phe-Phe- Ser-Ala-Gln at positions P2-P3', which is identical to the reactive center of human kallistatin. Rat kallistatin is highly similar to human kallistatin, sharing 68% and 57% sequence identity at the cDNA and the amino acid levels. The rat kallistatin gene exists in a single copy and is located on chromosome 6. An SphI RFLP is found between SHR and WKY rats at or near the rat kallistatin gene locus. Two amino acid polymorphisms of the rat kallistatin gene between these two strains were found by sequence analysis. A candidate promoter in the 5'-flanking region (109 bp) of the rat kallistatin gene has been identified by reporter assays. The expression of rat kallistatin in the liver is growth-dependent and down-regulated during acute phase inflammation. Recombinant rat kallistatin produced in E. coli is able to bind to tissue kallikrein, and the interaction is inhibited by heparin. These characteristics define rat kallistatin as the counterpart of human kallistatin.

AB - We have previously purified and cloned human kallistatin and rat kallikrein-binding protein (RKBP), which are tissue kallikrein inhibitors belonging to the serine proteinase inhibitor superfamily. In this study, we have cloned and sequenced the gene encoding rat kallistatin with Phe-Phe- Ser-Ala-Gln at positions P2-P3', which is identical to the reactive center of human kallistatin. Rat kallistatin is highly similar to human kallistatin, sharing 68% and 57% sequence identity at the cDNA and the amino acid levels. The rat kallistatin gene exists in a single copy and is located on chromosome 6. An SphI RFLP is found between SHR and WKY rats at or near the rat kallistatin gene locus. Two amino acid polymorphisms of the rat kallistatin gene between these two strains were found by sequence analysis. A candidate promoter in the 5'-flanking region (109 bp) of the rat kallistatin gene has been identified by reporter assays. The expression of rat kallistatin in the liver is growth-dependent and down-regulated during acute phase inflammation. Recombinant rat kallistatin produced in E. coli is able to bind to tissue kallikrein, and the interaction is inhibited by heparin. These characteristics define rat kallistatin as the counterpart of human kallistatin.

KW - (Rat)

KW - Gene cloning

KW - Kallikrein-binding protein

KW - Kallistatin

KW - Promoter activity

KW - Recombinant protein

KW - Serpin

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DO - 10.1016/S0167-4781(97)00100-0

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AN - SCOPUS:0030853582

SN - 0167-4781

VL - 1353

SP - 277

EP - 286

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 3

ER -

Molecular cloning and expression of rat kallistatin gene

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Keywords

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