Molecular cloning and expression of rat bradykinin B1 receptor

Aiguo Ni, Karl X. Chai, Lee Chao, Julie Chao

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

The gene encoding rat bradykinin B1 receptor has been cloned by using a partial rat B1 cDNA probe. The rat B1 receptor gene contains two exons and the entire coding region is within the second exon. The 5'-flanking region of the rat B1 receptor gene contains several putative transcriptional regulatory sites including TATA box, cAMP response element, NF-κB and AP-1. It showed promoter activity inducible by lipopolysaccharide in vascular smooth muscle cells. Rat B1 receptor mRNA was found to be alternatively spliced and induced by lipopolysaccharide treatment in a wide range of tissues, such as the salivary gland, testis, kidney, lung, heart, prostate and aorta. The deduced rat B1 receptor amino acid sequence is 71% homologous to human and rabbit counterparts, and 89% homologous to the mouse counterpart. The expressed B1 receptor in HEK293 cells displayed a rank order of affinity for the kinin peptides: des-Arg9-BK > Lys-des-Arg9-BK ≃ des-Arg9,Leu8-BK > Sar-Tyr-εAhx-Lys-[D-βNal7,Ile8]-des-Arg9-BK > Sar- Tyr-εAhx-Lys-des-Arg9-BK >> BK >> Hoe140. These results indicate that the cloned gene encodes a functional rat B1 receptor.

Original languageEnglish
Pages (from-to)177-185
Number of pages9
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1442
Issue number2-3
DOIs
StatePublished - 8 Nov 1998

Keywords

  • Alternative splicing
  • Bradykinin B receptor
  • Gene cloning
  • Inducible expression
  • Receptor binding

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