TY - JOUR
T1 - Molecular cloning and expression of rat bradykinin B1 receptor
AU - Ni, Aiguo
AU - Chai, Karl X.
AU - Chao, Lee
AU - Chao, Julie
N1 - Funding Information:
This work was supported by the National Institutes of Health grant HL52196.
PY - 1998/11/8
Y1 - 1998/11/8
N2 - The gene encoding rat bradykinin B1 receptor has been cloned by using a partial rat B1 cDNA probe. The rat B1 receptor gene contains two exons and the entire coding region is within the second exon. The 5'-flanking region of the rat B1 receptor gene contains several putative transcriptional regulatory sites including TATA box, cAMP response element, NF-κB and AP-1. It showed promoter activity inducible by lipopolysaccharide in vascular smooth muscle cells. Rat B1 receptor mRNA was found to be alternatively spliced and induced by lipopolysaccharide treatment in a wide range of tissues, such as the salivary gland, testis, kidney, lung, heart, prostate and aorta. The deduced rat B1 receptor amino acid sequence is 71% homologous to human and rabbit counterparts, and 89% homologous to the mouse counterpart. The expressed B1 receptor in HEK293 cells displayed a rank order of affinity for the kinin peptides: des-Arg9-BK > Lys-des-Arg9-BK ≃ des-Arg9,Leu8-BK > Sar-Tyr-εAhx-Lys-[D-βNal7,Ile8]-des-Arg9-BK > Sar- Tyr-εAhx-Lys-des-Arg9-BK >> BK >> Hoe140. These results indicate that the cloned gene encodes a functional rat B1 receptor.
AB - The gene encoding rat bradykinin B1 receptor has been cloned by using a partial rat B1 cDNA probe. The rat B1 receptor gene contains two exons and the entire coding region is within the second exon. The 5'-flanking region of the rat B1 receptor gene contains several putative transcriptional regulatory sites including TATA box, cAMP response element, NF-κB and AP-1. It showed promoter activity inducible by lipopolysaccharide in vascular smooth muscle cells. Rat B1 receptor mRNA was found to be alternatively spliced and induced by lipopolysaccharide treatment in a wide range of tissues, such as the salivary gland, testis, kidney, lung, heart, prostate and aorta. The deduced rat B1 receptor amino acid sequence is 71% homologous to human and rabbit counterparts, and 89% homologous to the mouse counterpart. The expressed B1 receptor in HEK293 cells displayed a rank order of affinity for the kinin peptides: des-Arg9-BK > Lys-des-Arg9-BK ≃ des-Arg9,Leu8-BK > Sar-Tyr-εAhx-Lys-[D-βNal7,Ile8]-des-Arg9-BK > Sar- Tyr-εAhx-Lys-des-Arg9-BK >> BK >> Hoe140. These results indicate that the cloned gene encodes a functional rat B1 receptor.
KW - Alternative splicing
KW - Bradykinin B receptor
KW - Gene cloning
KW - Inducible expression
KW - Receptor binding
UR - http://www.scopus.com/inward/record.url?scp=0032497830&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(98)00163-8
DO - 10.1016/S0167-4781(98)00163-8
M3 - Article
C2 - 9804950
AN - SCOPUS:0032497830
SN - 0167-4781
VL - 1442
SP - 177
EP - 185
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 2-3
ER -