2B4 is a 66 kD monomer expressed on the surface of all murine NK cells and the subset of T cells that mediate "non-MHC-restricted" cytotoxicity. Characterization of cDNA clones revealed that it is a novel member of the Immunoglobulin gene superfamily and showed homology to CD2, CD48, human LFA-3 and p58 molecules. Based on the cDNA sequence of 2B4, we have obtained two genomic clones from a mouse genomic library in the P1 system. Characterization of the two genomic clones revealed that one clone, 531 corresponds to the 2B4 cDNA whereas the other clone, 532 is a 2B4 related gene. In the present study we have determined the complete genomic structure of clone 532 and the expression pattern of 532 in different lymphocytes. Comparison of the coding regions of 531 and 532 showed 87% similarity. The expression pattern of clone 532 is similar to that of 531 in several mice strains tested. The functional significance of the novel gene is being investigated.
|State||Published - 1 Dec 1996|