TY - JOUR
T1 - Modulation of astrocyte phenotype in response to T-cell interaction
AU - Hersh, Jessica
AU - Prah, Jude
AU - Winters, Ali
AU - Liu, Ran
AU - Yang, Shao Hua
N1 - Funding Information:
This research was in part supported by National Institutes of Health grants R01NS088596 (SY), R01NS109583 (SY), T32 AG020494 (JH), and Osteopathic Scholars in Cancer Research RP170301 (JH). The authors declare no conflict of interest.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/2/15
Y1 - 2021/2/15
N2 - We determined that T-cell astrocyte interaction modulates interleukin-10 (IL-10) production from both cell types. The impact of IL-10 on astrocytes was compared to IL-10 generated from T-cell-astrocyte interactions in vitro. We demonstrated that T-cells directly interact with astrocytes to upregulate gene expression and secretion of IL-10, confirmed by elevated STAT3p/STAT3 expression in astrocytes. IL-10 increased astrocytes proliferation. In addition, IL-10 treatment and CD4+ co-culture shifts primary astrocytes toward a more energetic phenotype. These findings indicate that direct interaction of CD4+ T-cells with astrocytes, activated the IL-10 anti-inflammatory pathway, altering astrocyte phenotype, metabolism, and proliferation.
AB - We determined that T-cell astrocyte interaction modulates interleukin-10 (IL-10) production from both cell types. The impact of IL-10 on astrocytes was compared to IL-10 generated from T-cell-astrocyte interactions in vitro. We demonstrated that T-cells directly interact with astrocytes to upregulate gene expression and secretion of IL-10, confirmed by elevated STAT3p/STAT3 expression in astrocytes. IL-10 increased astrocytes proliferation. In addition, IL-10 treatment and CD4+ co-culture shifts primary astrocytes toward a more energetic phenotype. These findings indicate that direct interaction of CD4+ T-cells with astrocytes, activated the IL-10 anti-inflammatory pathway, altering astrocyte phenotype, metabolism, and proliferation.
UR - http://www.scopus.com/inward/record.url?scp=85098128183&partnerID=8YFLogxK
U2 - 10.1016/j.jneuroim.2020.577455
DO - 10.1016/j.jneuroim.2020.577455
M3 - Article
C2 - 33370671
AN - SCOPUS:85098128183
SN - 0165-5728
VL - 351
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
M1 - 577455
ER -